DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- T Efferth
- U Fabry
- R Osieka
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000167514600004&DestLinkType=FullRecord&DestApp=WOS_CPL
- Externe Identifier
- Clarivate Analytics Document Solution ID: 411TD
- PubMed Identifier: 11261779
- ISSN
- 0741-5400
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- JOURNAL OF LEUKOCYTE BIOLOGY
- Schlüsselwörter
- flow cytometry
- glutathione
- polymerase chain reaction
- NADPH
- Paginierung
- 340 - 342
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Titel
- DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation
- Sub types
- Article
- Ausgabe der Zeitschrift
- 69
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- Patients affected with X chromosome-linked, hereditary glucose-6-phosphate dehydrogenase (G6PD) deficiency suffer from life-threatening hemolytic crises after intake of certain drugs or foods. G6PD deficiency is associated with low levels of reduced glutathione. We analyzed mononuclear white blood cells (MNC) of three males suffering from the German G6PD Aachen variant, four heterozygote females of this family, one G6PD-deficient male from another family coming from Iran, and six healthy male volunteers with respect to their DNA damage in two different genes (G6PD and T-cell receptor-delta) and their propensity to enter apoptosis after UV illumination (0.08-5.28 J/cm2). As determined by PCR stop assays, there was more UV-induced DNA damage in MNC of G6PD-deficient male patients than in those of healthy subjects. MNC of G6PD-deficient patients showed a higher rate of apoptosis after UV irradiation than MNC of healthy donors. MNC of heterozygote females showed intermediate rates of DNA damage and apoptosis. It is concluded that increased DNA damage may be a result of deficient detoxification of reactive oxygen species by glutathione and may ultimately account for the higher rate of apoptosis in G6PD-deficient MNC.
- Addresses
- Department for Internal Medicine IV, University Hospital, Aachen, Germany.
- Autoren
- T Efferth
- U Fabry
- R Osieka
- eISSN
- 1938-3673
- Externe Identifier
- PubMed Identifier: 11261779
- Open access
- false
- ISSN
- 0741-5400
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Journal of leukocyte biology
- Schlüsselwörter
- Leukocytes, Mononuclear
- Humans
- Glucosephosphate Dehydrogenase Deficiency
- DNA Damage
- Glucosephosphate Dehydrogenase
- Glutathione
- DNA
- Polymerase Chain Reaction
- Ultraviolet Rays
- Apoptosis
- Heterozygote
- Genes, T-Cell Receptor delta
- Adolescent
- Adult
- Aged
- Middle Aged
- Female
- Male
- Genetic Variation
- Sprache
- eng
- Medium
- Paginierung
- 340 - 342
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Datum der Datenerfassung
- 2001
- Titel
- DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 69
Datenquelle: Europe PubMed Central
- Abstract
- Patients affected with X chromosome-linked, hereditary glucose-6-phosphate dehydrogenase (G6PD) deficiency suffer from life-threatening hemolytic crises after intake of certain drugs or foods. G6PD deficiency is associated with low levels of reduced glutathione. We analyzed mononuclear white blood cells (MNC) of three males suffering from the German G6PD Aachen variant, four heterozygote females of this family, one G6PD-deficient male from another family coming from Iran, and six healthy male volunteers with respect to their DNA damage in two different genes (G6PD and T-cell receptor-delta) and their propensity to enter apoptosis after UV illumination (0.08-5.28 J/cm2). As determined by PCR stop assays, there was more UV-induced DNA damage in MNC of G6PD-deficient male patients than in those of healthy subjects. MNC of G6PD-deficient patients showed a higher rate of apoptosis after UV irradiation than MNC of healthy donors. MNC of heterozygote females showed intermediate rates of DNA damage and apoptosis. It is concluded that increased DNA damage may be a result of deficient detoxification of reactive oxygen species by glutathione and may ultimately account for the higher rate of apoptosis in G6PD-deficient MNC.
- Autoren
- T Efferth
- U Fabry
- R Osieka
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/11261779
- ISSN
- 0741-5400
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- J Leukoc Biol
- Schlüsselwörter
- Adolescent
- Adult
- Aged
- Apoptosis
- DNA
- DNA Damage
- Female
- Genes, T-Cell Receptor delta
- Genetic Variation
- Glucosephosphate Dehydrogenase
- Glucosephosphate Dehydrogenase Deficiency
- Glutathione
- Heterozygote
- Humans
- Leukocytes, Mononuclear
- Male
- Middle Aged
- Polymerase Chain Reaction
- Ultraviolet Rays
- Sprache
- eng
- Country
- England
- Paginierung
- 340 - 342
- Datum der Veröffentlichung
- 2001
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2001
- Titel
- DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation.
- Sub types
- Comparative Study
- Journal Article
- Ausgabe der Zeitschrift
- 69
Datenquelle: PubMed
- Beziehungen:
- Eigentum von