DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation

Publication type:
Journal article
Metadata:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000167514600004&DestLinkType=FullRecord&DestApp=WOS_CPL
Externe Identifier
  • Clarivate Analytics Document Solution ID: 411TD
  • PubMed Identifier: 11261779
ISSN
0741-5400
Ausgabe der Veröffentlichung
3
Zeitschrift
JOURNAL OF LEUKOCYTE BIOLOGY
Schlüsselwörter
  • flow cytometry
  • glutathione
  • polymerase chain reaction
  • NADPH
Paginierung
340 - 342
Datum der Veröffentlichung
2001
Status
Published
Titel
DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation
Sub types
  • Article
Ausgabe der Zeitschrift
69

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
Patients affected with X chromosome-linked, hereditary glucose-6-phosphate dehydrogenase (G6PD) deficiency suffer from life-threatening hemolytic crises after intake of certain drugs or foods. G6PD deficiency is associated with low levels of reduced glutathione. We analyzed mononuclear white blood cells (MNC) of three males suffering from the German G6PD Aachen variant, four heterozygote females of this family, one G6PD-deficient male from another family coming from Iran, and six healthy male volunteers with respect to their DNA damage in two different genes (G6PD and T-cell receptor-delta) and their propensity to enter apoptosis after UV illumination (0.08-5.28 J/cm2). As determined by PCR stop assays, there was more UV-induced DNA damage in MNC of G6PD-deficient male patients than in those of healthy subjects. MNC of G6PD-deficient patients showed a higher rate of apoptosis after UV irradiation than MNC of healthy donors. MNC of heterozygote females showed intermediate rates of DNA damage and apoptosis. It is concluded that increased DNA damage may be a result of deficient detoxification of reactive oxygen species by glutathione and may ultimately account for the higher rate of apoptosis in G6PD-deficient MNC.
Addresses
  • Department for Internal Medicine IV, University Hospital, Aachen, Germany.
Autoren
eISSN
1938-3673
Externe Identifier
  • PubMed Identifier: 11261779
Open access
false
ISSN
0741-5400
Ausgabe der Veröffentlichung
3
Zeitschrift
Journal of leukocyte biology
Schlüsselwörter
  • Leukocytes, Mononuclear
  • Humans
  • Glucosephosphate Dehydrogenase Deficiency
  • DNA Damage
  • Glucosephosphate Dehydrogenase
  • Glutathione
  • DNA
  • Polymerase Chain Reaction
  • Ultraviolet Rays
  • Apoptosis
  • Heterozygote
  • Genes, T-Cell Receptor delta
  • Adolescent
  • Adult
  • Aged
  • Middle Aged
  • Female
  • Male
  • Genetic Variation
Sprache
eng
Medium
Print
Paginierung
340 - 342
Datum der Veröffentlichung
2001
Status
Published
Datum der Datenerfassung
2001
Titel
DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
69

Data source: Europe PubMed Central

Abstract
Patients affected with X chromosome-linked, hereditary glucose-6-phosphate dehydrogenase (G6PD) deficiency suffer from life-threatening hemolytic crises after intake of certain drugs or foods. G6PD deficiency is associated with low levels of reduced glutathione. We analyzed mononuclear white blood cells (MNC) of three males suffering from the German G6PD Aachen variant, four heterozygote females of this family, one G6PD-deficient male from another family coming from Iran, and six healthy male volunteers with respect to their DNA damage in two different genes (G6PD and T-cell receptor-delta) and their propensity to enter apoptosis after UV illumination (0.08-5.28 J/cm2). As determined by PCR stop assays, there was more UV-induced DNA damage in MNC of G6PD-deficient male patients than in those of healthy subjects. MNC of G6PD-deficient patients showed a higher rate of apoptosis after UV irradiation than MNC of healthy donors. MNC of heterozygote females showed intermediate rates of DNA damage and apoptosis. It is concluded that increased DNA damage may be a result of deficient detoxification of reactive oxygen species by glutathione and may ultimately account for the higher rate of apoptosis in G6PD-deficient MNC.
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11261779
ISSN
0741-5400
Ausgabe der Veröffentlichung
3
Zeitschrift
J Leukoc Biol
Schlüsselwörter
  • Adolescent
  • Adult
  • Aged
  • Apoptosis
  • DNA
  • DNA Damage
  • Female
  • Genes, T-Cell Receptor delta
  • Genetic Variation
  • Glucosephosphate Dehydrogenase
  • Glucosephosphate Dehydrogenase Deficiency
  • Glutathione
  • Heterozygote
  • Humans
  • Leukocytes, Mononuclear
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Ultraviolet Rays
Sprache
eng
Country
England
Paginierung
340 - 342
Datum der Veröffentlichung
2001
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2001
Titel
DNA damage and apoptosis in mononuclear cells from glucose-6-phosphate dehydrogenase-deficient patients (G6PD Aachen variant) after UV irradiation.
Sub types
  • Comparative Study
  • Journal Article
Ausgabe der Zeitschrift
69

Data source: PubMed

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