Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Mohamed EM Saeed
- Rolf Mertens
- Rupert Handgretinger
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000442971100026&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3892/ijo.2018.4491
- eISSN
- 1791-2423
- Externe Identifier
- Clarivate Analytics Document Solution ID: GR8JY
- PubMed Identifier: 30066889
- ISSN
- 1019-6439
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- INTERNATIONAL JOURNAL OF ONCOLOGY
- Schlüsselwörter
- biomarker
- hierarchical cluster analysis
- pediatric cancer
- tumor-infiltrating lymphocytes
- Paginierung
- 1721 - 1731
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling
- Sub types
- Article
- Ausgabe der Zeitschrift
- 53
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Mohamed Saeed
- Rolf Mertens
- Rupert Handgretinger
- Thomas Efferth
- DOI
- 10.3892/ijo.2018.4491
- eISSN
- 1791-2423
- ISSN
- 1019-6439
- Zeitschrift
- International Journal of Oncology
- Online publication date
- 2018
- Status
- Published online
- Herausgeber
- Spandidos Publications
- Herausgeber URL
- http://dx.doi.org/10.3892/ijo.2018.4491
- Datum der Datenerfassung
- 2020
- Titel
- Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling
Datenquelle: Crossref
- Abstract
- Nasopharyngeal carcinoma (NPC) is a rare disease in children with good prognosis and high cure rate. Nevertheless, certain patients have an unfavorable prognosis due to development of refractory NPC that is unresponsive to any therapeutic strategies. The current study studies a case of a 17 years-old female with non-keratinizing NPC type IIb (T2N0M0), who passed away as a consequence of resistance to chemo-, radio- and β-interferon therapy, and to an allogenic stem cell transplantation. In order to identify factors that lead to treatment failure and fatal outcome, immunohistochemical analyses of different tumor biomarkers and hierarchical cluster analysis were performed and compared with those of eight other patients with NPC who experienced complete remission following conventional therapy. Hierarchical cluster analysis of the immunohistochemical results clearly demonstrated that staining for immunological factors (CD4, CD8 and CD56) distinguished this patient from the others. To further investigate a potential role of the immune system, lymphocytic infiltration was assessed in tumor tissue by evaluation of hematoxylin and eosin-stained tumor sections. Indeed, no tumor infiltrating lymphocytes (TILs) were observed in this NPC case, while 7 out of 8 of the other NPC samples contained variable TIL amounts. The view that immunodeficiency of the patient may be a factor in the fatal outcome of treatment is supported by the fact that this patient with NPC was not positive for Epstein-Barr virus markers and also infected by several other viruses and fungi (herpes simplex virus, human herpes virus 6, Varicella zoster virus, and Candida). In conclusion, the investigation of rare NPC cases with poor prognosis may provide an improved understanding of the molecular mechanisms involved in refractory tumors and identification of novel potential therapeutic targets for NPC in the future.
- Addresses
- Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, D-55128 Mainz, Germany.
- Autoren
- Mohamed EM Saeed
- Rolf Mertens
- Rupert Handgretinger
- Thomas Efferth
- DOI
- 10.3892/ijo.2018.4491
- eISSN
- 1791-2423
- Externe Identifier
- PubMed Identifier: 30066889
- Open access
- false
- ISSN
- 1019-6439
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- International journal of oncology
- Schlüsselwörter
- Lymphocytes, Tumor-Infiltrating
- Humans
- Herpesvirus 4, Human
- Neoplasm Recurrence, Local
- Viral Matrix Proteins
- Prognosis
- Stem Cell Transplantation
- Immunohistochemistry
- Prospective Studies
- Gene Expression Profiling
- Immunocompromised Host
- Drug Resistance, Neoplasm
- Adolescent
- Child
- Female
- Male
- Biomarkers, Tumor
- Nasopharyngeal Carcinoma
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2018
- Paginierung
- 1721 - 1731
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum der Datenerfassung
- 2018
- Titel
- Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.
- Sub types
- Comparative Study
- Multicenter Study
- Journal Article
- Ausgabe der Zeitschrift
- 53
Datenquelle: Europe PubMed Central
- Abstract
- Nasopharyngeal carcinoma (NPC) is a rare disease in children with good prognosis and high cure rate. Nevertheless, certain patients have an unfavorable prognosis due to development of refractory NPC that is unresponsive to any therapeutic strategies. The current study studies a case of a 17 years-old female with non-keratinizing NPC type IIb (T2N0M0), who passed away as a consequence of resistance to chemo-, radio- and β-interferon therapy, and to an allogenic stem cell transplantation. In order to identify factors that lead to treatment failure and fatal outcome, immunohistochemical analyses of different tumor biomarkers and hierarchical cluster analysis were performed and compared with those of eight other patients with NPC who experienced complete remission following conventional therapy. Hierarchical cluster analysis of the immunohistochemical results clearly demonstrated that staining for immunological factors (CD4, CD8 and CD56) distinguished this patient from the others. To further investigate a potential role of the immune system, lymphocytic infiltration was assessed in tumor tissue by evaluation of hematoxylin and eosin-stained tumor sections. Indeed, no tumor infiltrating lymphocytes (TILs) were observed in this NPC case, while 7 out of 8 of the other NPC samples contained variable TIL amounts. The view that immunodeficiency of the patient may be a factor in the fatal outcome of treatment is supported by the fact that this patient with NPC was not positive for Epstein-Barr virus markers and also infected by several other viruses and fungi (herpes simplex virus, human herpes virus 6, Varicella zoster virus, and Candida). In conclusion, the investigation of rare NPC cases with poor prognosis may provide an improved understanding of the molecular mechanisms involved in refractory tumors and identification of novel potential therapeutic targets for NPC in the future.
- Date of acceptance
- 2018
- Autoren
- Mohamed EM Saeed
- Rolf Mertens
- Rupert Handgretinger
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30066889
- DOI
- 10.3892/ijo.2018.4491
- eISSN
- 1791-2423
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Int J Oncol
- Schlüsselwörter
- Adolescent
- Biomarkers, Tumor
- Child
- Drug Resistance, Neoplasm
- Female
- Gene Expression Profiling
- Herpesvirus 4, Human
- Humans
- Immunocompromised Host
- Immunohistochemistry
- Lymphocytes, Tumor-Infiltrating
- Male
- Nasopharyngeal Carcinoma
- Neoplasm Recurrence, Local
- Prognosis
- Prospective Studies
- Stem Cell Transplantation
- Viral Matrix Proteins
- Sprache
- eng
- Country
- Greece
- Paginierung
- 1721 - 1731
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.
- Sub types
- Comparative Study
- Journal Article
- Multicenter Study
- Ausgabe der Zeitschrift
- 53
Datenquelle: PubMed
- Beziehungen:
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