Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling

Autoren

Mohamed Saeed
Rolf Mertens
Rupert Handgretinger
Thomas Efferth

DOI

10.3892/ijo.2018.4491

eISSN

1791-2423

ISSN

1019-6439

Zeitschrift

International Journal of Oncology

Online publication date

2018

Status

Published online

Herausgeber

Spandidos Publications

Herausgeber URL

http://dx.doi.org/10.3892/ijo.2018.4491

Datum der Datenerfassung

2020

Titel

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling

Data source: Crossref

Abstract

Nasopharyngeal carcinoma (NPC) is a rare disease in children with good prognosis and high cure rate. Nevertheless, certain patients have an unfavorable prognosis due to development of refractory NPC that is unresponsive to any therapeutic strategies. The current study studies a case of a 17 years-old female with non-keratinizing NPC type IIb (T2N0M0), who passed away as a consequence of resistance to chemo-, radio- and β-interferon therapy, and to an allogenic stem cell transplantation. In order to identify factors that lead to treatment failure and fatal outcome, immunohistochemical analyses of different tumor biomarkers and hierarchical cluster analysis were performed and compared with those of eight other patients with NPC who experienced complete remission following conventional therapy. Hierarchical cluster analysis of the immunohistochemical results clearly demonstrated that staining for immunological factors (CD4, CD8 and CD56) distinguished this patient from the others. To further investigate a potential role of the immune system, lymphocytic infiltration was assessed in tumor tissue by evaluation of hematoxylin and eosin-stained tumor sections. Indeed, no tumor infiltrating lymphocytes (TILs) were observed in this NPC case, while 7 out of 8 of the other NPC samples contained variable TIL amounts. The view that immunodeficiency of the patient may be a factor in the fatal outcome of treatment is supported by the fact that this patient with NPC was not positive for Epstein-Barr virus markers and also infected by several other viruses and fungi (herpes simplex virus, human herpes virus 6, Varicella zoster virus, and Candida). In conclusion, the investigation of rare NPC cases with poor prognosis may provide an improved understanding of the molecular mechanisms involved in refractory tumors and identification of novel potential therapeutic targets for NPC in the future.

Addresses

Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, D-55128 Mainz, Germany.

Autoren

Mohamed EM Saeed
Rolf Mertens
Rupert Handgretinger
Thomas Efferth

DOI

10.3892/ijo.2018.4491

eISSN

1791-2423

Externe Identifier

PubMed Identifier: 30066889

Open access

false

ISSN

1019-6439

Ausgabe der Veröffentlichung

4

Zeitschrift

International journal of oncology

Schlüsselwörter

Lymphocytes, Tumor-Infiltrating
Humans
Herpesvirus 4, Human
Neoplasm Recurrence, Local
Viral Matrix Proteins
Prognosis
Stem Cell Transplantation
Immunohistochemistry
Prospective Studies
Gene Expression Profiling
Immunocompromised Host
Drug Resistance, Neoplasm
Adolescent
Child
Female
Male
Biomarkers, Tumor
Nasopharyngeal Carcinoma

Sprache

eng

Medium

Print-Electronic

Online publication date

2018

Paginierung

1721 - 1731

Datum der Veröffentlichung

2018

Status

Published

Datum der Datenerfassung

2018

Titel

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

Sub types

Comparative Study
Multicenter Study
Journal Article

Ausgabe der Zeitschrift

53

Data source: Europe PubMed Central

Abstract

Nasopharyngeal carcinoma (NPC) is a rare disease in children with good prognosis and high cure rate. Nevertheless, certain patients have an unfavorable prognosis due to development of refractory NPC that is unresponsive to any therapeutic strategies. The current study studies a case of a 17 years-old female with non-keratinizing NPC type IIb (T2N0M0), who passed away as a consequence of resistance to chemo-, radio- and β-interferon therapy, and to an allogenic stem cell transplantation. In order to identify factors that lead to treatment failure and fatal outcome, immunohistochemical analyses of different tumor biomarkers and hierarchical cluster analysis were performed and compared with those of eight other patients with NPC who experienced complete remission following conventional therapy. Hierarchical cluster analysis of the immunohistochemical results clearly demonstrated that staining for immunological factors (CD4, CD8 and CD56) distinguished this patient from the others. To further investigate a potential role of the immune system, lymphocytic infiltration was assessed in tumor tissue by evaluation of hematoxylin and eosin-stained tumor sections. Indeed, no tumor infiltrating lymphocytes (TILs) were observed in this NPC case, while 7 out of 8 of the other NPC samples contained variable TIL amounts. The view that immunodeficiency of the patient may be a factor in the fatal outcome of treatment is supported by the fact that this patient with NPC was not positive for Epstein-Barr virus markers and also infected by several other viruses and fungi (herpes simplex virus, human herpes virus 6, Varicella zoster virus, and Candida). In conclusion, the investigation of rare NPC cases with poor prognosis may provide an improved understanding of the molecular mechanisms involved in refractory tumors and identification of novel potential therapeutic targets for NPC in the future.

Date of acceptance

2018

Autoren

Mohamed EM Saeed
Rolf Mertens
Rupert Handgretinger
Thomas Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/30066889

DOI

10.3892/ijo.2018.4491

eISSN

1791-2423

Ausgabe der Veröffentlichung

4

Zeitschrift

Int J Oncol

Schlüsselwörter

Adolescent
Biomarkers, Tumor
Child
Drug Resistance, Neoplasm
Female
Gene Expression Profiling
Herpesvirus 4, Human
Humans
Immunocompromised Host
Immunohistochemistry
Lymphocytes, Tumor-Infiltrating
Male
Nasopharyngeal Carcinoma
Neoplasm Recurrence, Local
Prognosis
Prospective Studies
Stem Cell Transplantation
Viral Matrix Proteins

Sprache

eng

Country

Greece

Paginierung

1721 - 1731

Datum der Veröffentlichung

2018

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2018

Titel

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

Sub types

Comparative Study
Journal Article
Multicenter Study

Ausgabe der Zeitschrift

53

Data source: PubMed

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling

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