Edward Lemke

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• Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells
• Synthese, Biochemische Charakterisierung und Genetische Kodierung einer 1,2,4‐Triazol‐Aminosäure als Acetyllysin‐Mimetikum für Bromodomänen der BET‐Familie
• Mechanismusabhängige Regulation der ultraschnellen Dynamik von Wasser an Grenzflächen in Komplexen mit intrinsisch ungeordneten Proteinen
• Verbesserte Erweiterung des eukaryotischen genetischen Codes für seitenspezifische, hochauflösende Click‐PAINT‐Mikroskopie
• Titelbild: Verbesserte Erweiterung des eukaryotischen genetischen Codes für seitenspezifische, hochauflösende Click‐PAINT‐Mikroskopie (Angew. Chem. 52/2016)
• Protein-specific crowding accelerates aging in phase-separated droplets
• Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities
• Beyond the Transport Function of Import Receptors: What's All the FUS about?
• Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"
• Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids
• Shining a Light on Phase Separation in the Cell
• Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α
• Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields
• Special Issue: Expanding the Genetic Code
• The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor
• Mapping Multivalency and Differential Affinities within Large Intrinsically Disordered Protein Complexes with Segmental Motion Analysis
• Principles for designing fluorescent sensors and reporters
• MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
• Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
• Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study (vol 15, pg 984, 2018)
• Sampling Long- versus Short-Range Interactions Defines the Ability of Force Fields To Reproduce the Dynamics of Intrinsically Disordered Proteins
• Precision Control of Cellular Pathways with Light
• What precision-protein-tuning and nano-resolved single molecule sciences can do for each other
• Decoding molecular plasticity of the dark proteome
• Synthesis of Azido-Glycans for Chemical Glycomodification of Proteins
• Decoding molecular plasticity in the dark proteome
• Shining Light on the 'Dark Matter' of the Nuclear Pore Complex
• Versatile Sensor Design in Cellulo by Combining Membraneless Organelles with Click Chemistry
• Superresolution Mapping of Intrinsically Disordered Regions of Nucleoporins In Situ
• In Cell Kinetic FRET Assay to Judge Suitability of Bioorthogonal Dye Labelling Reaction
• Molecular Determinants of Large Cargo Transport into the Nucleus
• Deciphering the structure and function of FG-nucleoporins in situ using FLIM-FRET
• Synthetic membraneless organelles enable highly selective protein labeling for time-resolved live-cell imaging
• Rescuing the Over-Collapse of Intrinsically Disordered Proteins using a Force Field Derived by a New Paradigm
• Site-Specific Labeling of Proteins in Live Mammalian Cells using Click Chemistry
• A New Tool for Custom Protein Design and Engineering-DH10 Bac-TAG
• Decoding Protein Plasticity from Single Molecules to Large Complexes
• Single-molecule FRET reveals proline dynamics in transmembrane helices
• Conformational Dynamics of Molecular Chaperones Investigated by Single Molecule Multicolor Forster Resonance Energy Transfer
• Dissecting the Nuclear Pore-Like Permeability Barrier Function of Phase Separated Liquid FG Nucleoporin Condensates
• Designer Membraneless Organelles Enable Highly Specific Protein Engineering in Eukaryotes
• Deciphering the Conformational State of FG-Nucleoporins In Situ
• Sequence-Encoded Heterogeneity of Interactions Decouples Different Measures of Protein Sizes and Reconciles the Discrepant Inferences from Saxs versus Fret Experiments
• Noncanonical Amino Acids Methods and Protocols Preface
• Incorporating Single-Particle Biophysics into Correlated Cryo-Fluorescence and Cryo-Electron Microscopy
• Following a Giant's Footsteps: Single-Particle and Super-Resolution Approaches to Decipher the Nuclear Transport of Hepatitis B Virus Capsids
• Decoding Molecular Plasticity Underlying Nucleocytoplasmic Transport: from Single Molecules to Large Assemblies
• Labeling Freedom for the Single Molecule Microscopist
• Absolute Stoichiometry of the Nuclear Pore Complex
• Long-Term Single-Molecule TIRF Observation of Biomolecules without Immobilization
• Nup153 in Nuclear Transport: Plasticity of Nucleoporin/Transport-Receptor Complexes
• Labeling Freedom for the Single Molecule Microscopist
• Long Term Single Molecule TIRF Observation of Biomolecules without Immobilization
• A New and Un-Conventional Ultrafast Binding Mechanism of Intrinsically Disordered Proteins to Structured Partners
• Continuous throughput and long-term observation of single-molecule FRET without immobilization for studying protein dynamics
• Prion conformational dynamics at single-molecule resolution
• Visualization of single synaptic vesicle dynamics in hippocampal boutons by two-photon laser-scanning microscopy
• Genetically Encoded Click Chemistry for Single-Molecule FRET of Proteins
• Comparative analysis of the coordinated motion of Hsp70s from different organelles observed by single-molecule three-color FRET
• High-Resolution smFRET in a Microfluidic Gas Exchange Platform
• Single Molecule Study of the Intrinsically Disordered FG-repeat Nucleoporin 153
• Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation
• Application of Noncanonical Amino Acids for Protein Labeling in a Genomically Recoded Escherichia coli
• Bistetrazine-Cyanines as Double-Clicking Fluorogenic Two-Point Binder or Crosslinker Probes
• Polyglutamine Length Dependent Structural Properties and Phase Behavior of Huntingtin Exon 1
• Genetic code expansion for multiprotein complex engineering
• Comparative analysis of the coordinated motion of Hsp70s from different organelles observed by single-molecule three-color FRET
• Plasticity of Nucleoporin Nuclear Transport Receptor Interactions - Molecular Description of a Highly Dynamic, Ultrafast Interaction Mechanism
• Interplay Between Surface Solvation and Molecular Recognition in IDPs
• Interplay Between Surface Solvation and Molecular Recognition in IDPs
• Surface Solvation Tunes Molecular-Recognition Plasticity in IDPs
• Dissecting Conformational Features and Binding Mechanisms of Phenylalanine-Glycine Rich Nucleoporins
• Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification
• A new family of bioorthogonally applicable fluorogenic labels
• Continuous throughput and long-term observation of single-molecule FRET without immobilization
• Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers
• Single-molecule biophysics: at the interface of biology, physics and chemistry
• Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity
• Nanoscale devices for linkerless long-term single-molecule observation
• Stressing the role of a short linear motif in ataxin-2 condensation
• Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors
• Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli
• Visualizing the disordered nuclear transport machinery in situ
• Origin of Orthogonality of Strain-Promoted Click Reactions
• Labeling of virus components for advanced, quantitative imaging analyses
• Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy
• Amino Acids for Diels-Alder Reactions in Living Cells
• Single Molecule Study of the Intrinsically Disordered FG-Repeat Nucleoporin 153
• Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths
• Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements
• A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility
• Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies
• Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors
• Click Strategies for Single-Molecule Protein Fluorescence
• Genetically Encoded Copper-Free Click Chemistry
• Adding intrinsically disordered proteins to biological ageing clocks
• Fourier ring correlation as a resolution criterion for super-resolution microscopy
• A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
• Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines
• Facilitated aggregation of FG nucleoporins under molecular crowding conditions
• Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range
• Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy
• Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy
• Hydrophilic trans-Cyclooctenylated Noncanonical Amino Acids for Fast Intracellular Protein Labeling
• New Red-Emitting Tetrazine-Phenoxazine Fluorogenic Labels for Live-Cell Intracellular Bioorthogonal Labeling Schemes
• The Multiple Faces of Disordered Nucleoporins
• In situ structural analysis of the human nuclear pore complex
• Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond
• Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling
• Labeling proteins on live mammalian cells using click chemistry
• The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
• Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET
• Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET
• Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study
• Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy
• FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
• Physics of the nuclear pore complex: Theory, modeling and experiment
• Synthesis and Evaluation of Novel Ring-Strained Noncanonical Amino Acids for Residue-Specific Bioorthogonal Reactions in Living Cells
• PED in 2021: a major update of the protein ensemble database for intrinsically disordered proteins
• Multifunctionality of F-rich nucleoporins
• Cargo transport through the nuclear pore complex at a glance
• Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing
• Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins in Saccharomyces cerevisiae
• Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability
• Direct single-molecule observation of a protein living in two opposed native structures
• Interplay of α-synuclein binding and conformational switching probed by single-molecule fluorescence
• A General and Efficient Method for the Site-Specific Dual-Labeling of Proteins for Single Molecule Fluorescence Resonance Energy Transfer
• Control of protein phosphorylation with a genetically encoded photocaged amino acid
• A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures
• Molecular anatomy of a trafficking organelle
• Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins
• Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
• Bioorthogonal red and far-red fluorogenic probes for wash-free live-cell and super-resolution microscopy
• Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins
• Raising the ribosomal repertoire
• Mechanism-Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes
• Inducible Genetic Code Expansion in Eukaryotes
• Molecular determinants of large cargo transport into the nucleus
• Phase Separation Comes of Age: From Phenomenology to Single Molecules
• Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes
• Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors
• Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-Cyclooctynylated Peptide Tags and as Fluorogenic Cross-Linkers of Cyclooctynylated Proteins
• Orthogonal spin labeling using click chemistry for in vitro and in vivo applications
• Genetic Code Expansion for Site-Specific Labeling of Antibodies with Radioisotopes
• Molecular determinants of large cargo transport into the nucleus
• Deciphering the conformations and dynamics of FG-nucleoporins in situ
• Unnatural Amino Acid Mutagenesis Reveals Dimerization As a Negative Regulatory Mechanism of VHR's Phosphatase Activity
• Conserved features of intermediates in amyloid assembly determine their benign or toxic states
• Immune complexes of auto-antibodies against Aβ1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients
• Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy
• Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)
• Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state in General Transcription Factor TFIID function
• Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes
• Synthesis, Biochemical Characterization, and Genetic Encoding of a 1,2,4-Triazole Amino Acid as an Acetyllysine Mimic for Bromodomains of the BET Family
• Condensed, Microtubule-coating Thin Organelles for Orthogonal Translation in Mammalian Cells
• Role of Solvent Compatibility in the Phase Behavior of Binary Solutions of Weakly Associating Multivalent Polymers
• When two become one: Integrating FRET and EPR into one structural model
• Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation
• Synthetic biomolecular condensates to engineer eukaryotic cells
• Faces, facets, and functions of biomolecular condensates driven by multivalent proteins and nucleic acids
• There is plenty of room in protein-RNA condensates
• New Generation of Bioorthogonally Applicable Fluorogenic Dyes with Visible Excitations and Large Stokes Shifts
• Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
• Role of Solvent Compatibility in the Phase Behavior of Binary Solutions of Weakly Associating Multivalent Polymers