Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000359032300011&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.sbi.2015.02.009
eISSN
1879-033X
Externe Identifier
  • Clarivate Analytics Document Solution ID: CO3BT
  • PubMed Identifier: 25757192
ISSN
0959-440X
Zeitschrift
CURRENT OPINION IN STRUCTURAL BIOLOGY
Paginierung
66 - 73
Datum der Veröffentlichung
2015
Status
Published
Titel
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids
Sub types
  • Article
Ausgabe der Zeitschrift
32

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
DOI
10.1016/j.sbi.2015.02.009
ISSN
0959-440X
Zeitschrift
Current Opinion in Structural Biology
Sprache
en
Paginierung
66 - 73
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.sbi.2015.02.009
Datum der Datenerfassung
2022
Titel
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids
Ausgabe der Zeitschrift
32

Datenquelle: Crossref

Abstract
Contemporary structural biology research promises more than just static snap-shots of molecular machineries. This goal is not just facilitated by combining different structural biology techniques, but also by new tools from the field of protein and genetic engineering, as well as from chemistry. Genetic encoding of noncanonical amino acids (ncAAs) through codon-suppression technology provides an excellent opportunity to probe biomolecules using different structural biology methods. In this article, we review the applications of ncAA incorporation into proteins for determining structural information through various techniques with the main focus on crosslinking mass spectrometry and single-molecule FRET-based techniques. Furthermore, advances and limitations of the incorporation of multiple ncAAs are discussed, with respect to design of an ideal host organism for modern and integrative structural biology research.
Addresses
  • Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Autoren
DOI
10.1016/j.sbi.2015.02.009
eISSN
1879-033X
Externe Identifier
  • PubMed Identifier: 25757192
Funding acknowledgements
  • DFG: SPP1623
  • Emmy Noether Programme:
Open access
false
ISSN
0959-440X
Zeitschrift
Current opinion in structural biology
Schlüsselwörter
  • Animals
  • Humans
  • Amino Acids
  • Proteins
  • Cross-Linking Reagents
  • Fluorescence Resonance Energy Transfer
  • Genetic Engineering
  • Protein Conformation
  • Genetic Code
  • Models, Molecular
  • Mass Spectrometry
  • Synthetic Biology
Sprache
eng
Medium
Print-Electronic
Online publication date
2015
Paginierung
66 - 73
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids.
Sub types
  • Research Support, Non-U.S. Gov't
  • Review
  • Journal Article
Ausgabe der Zeitschrift
32

Datenquelle: Europe PubMed Central

Abstract
Contemporary structural biology research promises more than just static snap-shots of molecular machineries. This goal is not just facilitated by combining different structural biology techniques, but also by new tools from the field of protein and genetic engineering, as well as from chemistry. Genetic encoding of noncanonical amino acids (ncAAs) through codon-suppression technology provides an excellent opportunity to probe biomolecules using different structural biology methods. In this article, we review the applications of ncAA incorporation into proteins for determining structural information through various techniques with the main focus on crosslinking mass spectrometry and single-molecule FRET-based techniques. Furthermore, advances and limitations of the incorporation of multiple ncAAs are discussed, with respect to design of an ideal host organism for modern and integrative structural biology research.
Date of acceptance
2015
Autoren
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/25757192
DOI
10.1016/j.sbi.2015.02.009
eISSN
1879-033X
Zeitschrift
Curr Opin Struct Biol
Schlüsselwörter
  • Amino Acids
  • Animals
  • Cross-Linking Reagents
  • Fluorescence Resonance Energy Transfer
  • Genetic Code
  • Genetic Engineering
  • Humans
  • Mass Spectrometry
  • Models, Molecular
  • Protein Conformation
  • Proteins
  • Synthetic Biology
Sprache
eng
Country
England
Paginierung
66 - 73
PII
S0959-440X(15)00018-4
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review
Ausgabe der Zeitschrift
32

Datenquelle: PubMed

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