MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
- Publikationstyp:
- Kapitel
- Metadaten:
-
- Autoren
- Christine Koehler
- Edward A Lemke
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000434794700020&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1007/978-1-4939-7574-7_19
- ISBN-13
- 978-1-4939-7573-0
- Schlüsselwörter
- MultiBac
- Baculovirus
- Genetic code expansion
- Noncanonical amino acids
- Paginierung
- 297 - 311
- Buchtitel
- NONCANONICAL AMINO ACIDS: METHODS AND PROTOCOLS
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Titel
- MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
- Ausgabe der Zeitschrift
- 1728
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Christine Koehler
- Edward A Lemke
- DOI
- 10.1007/978-1-4939-7574-7_19
- ISBN-13
- 9781493975730
- Online publication date
- 2018
- Paginierung
- 297 - 311
- Buchtitel
- Methods in Molecular Biology
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Herausgeber
- Springer New York
- Herausgeber URL
- http://dx.doi.org/10.1007/978-1-4939-7574-7_19
- Datum der Datenerfassung
- 2019
- Titel
- MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
Datenquelle: Crossref
- Abstract
- The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids.
- Addresses
- Structural and Computational Biology Unit & Cell Biology and Biophysics Unit, EMBL, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
- Autoren
- Christine Koehler
- Edward A Lemke
- DOI
- 10.1007/978-1-4939-7574-7_19
- Open access
- false
- Schlüsselwörter
- Animals
- Escherichia coli
- Baculoviridae
- Amino Acids
- Gene Transfer Techniques
- Transformation, Bacterial
- Gene Expression
- Recombination, Genetic
- Genetic Code
- Genetic Vectors
- Plasmids
- Sf9 Cells
- Medium
- Paginierung
- 297 - 311
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum der Datenerfassung
- 2018
- Titel
- MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells.
- Ausgabe der Zeitschrift
- 1728
Datenquelle: Europe PubMed Central
- Abstract
- The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids.
- Autoren
- Christine Koehler
- Edward A Lemke
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/29405006
- DOI
- 10.1007/978-1-4939-7574-7_19
- Schlüsselwörter
- Baculovirus
- Genetic code expansion
- MultiBac
- Noncanonical amino acids
- Amino Acids
- Animals
- Baculoviridae
- Escherichia coli
- Gene Expression
- Gene Transfer Techniques
- Genetic Code
- Genetic Vectors
- Plasmids
- Recombination, Genetic
- Sf9 Cells
- Transformation, Bacterial
- Paginierung
- 297 - 311
- Datum der Veröffentlichung
- 2018
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells.
- Ausgabe der Zeitschrift
- 1728
Datenquelle: PubMed
- Beziehungen:
- Eigentum von