Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins

Publikationstyp:
Kapitel
Metadaten:
Autoren
  • Eszter Kozma
  • Giulia Paci
  • Gemma Estrada Girona
  • Edward A Lemke
  • Peter Kele
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000434794700023&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/978-1-4939-7574-7_22
ISBN-13
978-1-4939-7573-0
Schlüsselwörter
  • Bioorthogonality
  • Tetrazine
  • Fluorogenicity
  • NIR emission
  • Super-resolution microscopy
Paginierung
337 - 363
Buchtitel
NONCANONICAL AMINO ACIDS: METHODS AND PROTOCOLS
Datum der Veröffentlichung
2018
Status
Published
Titel
Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
Ausgabe der Zeitschrift
1728

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Autoren
  • Eszter Kozma
  • Giulia Paci
  • Gemma Estrada Girona
  • Edward A Lemke
  • Péter Kele
DOI
10.1007/978-1-4939-7574-7_22
ISBN-13
9781493975730
Online publication date
2018
Paginierung
337 - 363
Buchtitel
Methods in Molecular Biology
Datum der Veröffentlichung
2018
Status
Published
Herausgeber
Springer New York
Herausgeber URL
http://dx.doi.org/10.1007/978-1-4939-7574-7_22
Datum der Datenerfassung
2019
Titel
Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins

Datenquelle: Crossref

Abstract
Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells.
Addresses
  • Chemical Biology Research Group, Research Centre for Natural Sciences, Institute of Organic Chemistry, Hungarian Academy of Sciences, Magyar tudósok krt 2, Budapest, 1117, Hungary.
Autoren
  • Eszter Kozma
  • Giulia Paci
  • Gemma Estrada Girona
  • Edward A Lemke
  • Péter Kele
DOI
10.1007/978-1-4939-7574-7_22
Open access
false
Schlüsselwörter
  • COS Cells
  • Animals
  • Humans
  • Silicon
  • Rhodamines
  • Amino Acids
  • Proteins
  • Fluorescent Dyes
  • Microscopy, Fluorescence
  • Staining and Labeling
  • Transfection
  • Chlorocebus aethiops
Medium
Print
Paginierung
337 - 363
Datum der Veröffentlichung
2018
Status
Published
Datum der Datenerfassung
2018
Titel
Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins.
Ausgabe der Zeitschrift
1728

Datenquelle: Europe PubMed Central

Abstract
Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells.
Autoren
  • Eszter Kozma
  • Giulia Paci
  • Gemma Estrada Girona
  • Edward A Lemke
  • Péter Kele
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/29405009
DOI
10.1007/978-1-4939-7574-7_22
Schlüsselwörter
  • Bioorthogonality
  • Fluorogenicity
  • NIR emission
  • Super-resolution microscopy
  • Tetrazine
  • Amino Acids
  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Fluorescent Dyes
  • Humans
  • Microscopy, Fluorescence
  • Proteins
  • Rhodamines
  • Silicon
  • Staining and Labeling
  • Transfection
Paginierung
337 - 363
Datum der Veröffentlichung
2018
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins.
Ausgabe der Zeitschrift
1728

Datenquelle: PubMed

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