Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells

Publication type:
Journal article
Metadata:
Autoren
  • Uwe Wolfrum
  • Angelika Schmitt
Sammlungen
  • metadata
ISSN
0886-1544
Ausgabe der Veröffentlichung
2
Zeitschrift
Cell motility and the cytoskeleton
Schlüsselwörter
  • 590 Tiere (Zoologie)
  • 590 Zoological sciences
Sprache
eng
Paginierung
Seiten: 95 - 107
Datum der Veröffentlichung
2000
Herausgeber
Wiley-Blackwell
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells
Ausgabe der Zeitschrift
46

Data source: METADATA.UB

Other metadata sources:
Autoren
  • U Wolfrum
  • A Schmitt
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000088077100002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/1097-0169(200006)46:2<95::AID-CM2>3.0.CO;2-Q
Externe Identifier
  • Clarivate Analytics Document Solution ID: 332MB
  • PubMed Identifier: 10891855
ISSN
0886-1544
Ausgabe der Veröffentlichung
2
Zeitschrift
CELL MOTILITY AND THE CYTOSKELETON
Schlüsselwörter
  • photoreceptor
  • rhodopsin
  • cilium
  • myosin
  • actin
  • Usher Syndrome
  • ciliary transport
Paginierung
95 - 107
Datum der Veröffentlichung
2000
Status
Published
Titel
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells
Sub types
  • Article
Ausgabe der Zeitschrift
46

Data source: Web of Science (Lite)

Autoren
  • Uwe Wolfrum
  • Angelika Schmitt
DOI
10.1002/1097-0169(200006)46:2<95::aid-cm2>3.0.co;2-q
eISSN
1097-0169
ISSN
0886-1544
Ausgabe der Veröffentlichung
2
Zeitschrift
Cell Motility and the Cytoskeleton
Sprache
en
Online publication date
2000
Paginierung
95 - 107
Datum der Veröffentlichung
2000
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/1097-0169(200006)46:2%3C95::aid-cm2%3E3.0.co;2-q
Datum der Datenerfassung
2023
Titel
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells
Ausgabe der Zeitschrift
46

Data source: Crossref

Abstract
The transport of the photopigment rhodopsin from the inner segment to the photosensitive outer segment of vertebrate photoreceptor cells has been one of the main remaining mysteries in photoreceptor cell biology. Because of the lack of any direct evidence for the pathway through the photoreceptor cilium, alternative extracellular pathways have been proposed. Our primary aim in the present study was to resolve rhodopsin trafficking from the inner to the outer segment. We demonstrate, predominantly by high-sensitive immunoelectron microscopy, that rhodopsin is also densely packed in the membrane of the photoreceptor connecting cilium. Present prominent labeling of rhodopsin in the ciliary membrane provides the first striking evidence that rhodopsin is translocated from the inner segment to the outer segment of wild type photoreceptors via the ciliary membrane. At the ciliary membrane rhodopsin co-localizes with the unconventional myosin VIIa, the product of human Usher syndrome 1B gene. Furthermore, axonemal actin was identified in the photoreceptor cilium, which is spatially co-localized with myosin VIIa and opsin. This actin cytoskeleton of the cilium may provide the structural bases for myosin VIIa-linked ciliary trafficking of membrane components, including rhodopsin.
Addresses
  • Institute of Zoology, Johannes Gutenberg-University of Mainz, Mainz, Germany. wolfrum@mail.uni-mainz.de
Autoren
  • U Wolfrum
  • A Schmitt
DOI
10.1002/1097-0169(200006)46:2<95::aid-cm2>3.0.co;2-q
eISSN
1097-0169
Externe Identifier
  • PubMed Identifier: 10891855
Open access
false
ISSN
0886-1544
Ausgabe der Veröffentlichung
2
Zeitschrift
Cell motility and the cytoskeleton
Schlüsselwörter
  • Cilia
  • Animals
  • Mice, Inbred C57BL
  • Cattle
  • Humans
  • Mice
  • Rats
  • Rats, Sprague-Dawley
  • Retinitis Pigmentosa
  • Actins
  • Myosins
  • Rhodopsin
  • Antibodies, Monoclonal
  • Microscopy, Immunoelectron
  • Immunoblotting
  • Immunohistochemistry
  • Amino Acid Sequence
  • Biological Transport
  • Molecular Sequence Data
  • Middle Aged
  • Female
  • Rod Cell Outer Segment
  • Rod Opsins
  • Retinal Rod Photoreceptor Cells
  • Dyneins
  • Myosin VIIa
Sprache
eng
Medium
Print
Paginierung
95 - 107
Datum der Veröffentlichung
2000
Status
Published
Datum der Datenerfassung
2000
Titel
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
46

Data source: Europe PubMed Central

Abstract
The transport of the photopigment rhodopsin from the inner segment to the photosensitive outer segment of vertebrate photoreceptor cells has been one of the main remaining mysteries in photoreceptor cell biology. Because of the lack of any direct evidence for the pathway through the photoreceptor cilium, alternative extracellular pathways have been proposed. Our primary aim in the present study was to resolve rhodopsin trafficking from the inner to the outer segment. We demonstrate, predominantly by high-sensitive immunoelectron microscopy, that rhodopsin is also densely packed in the membrane of the photoreceptor connecting cilium. Present prominent labeling of rhodopsin in the ciliary membrane provides the first striking evidence that rhodopsin is translocated from the inner segment to the outer segment of wild type photoreceptors via the ciliary membrane. At the ciliary membrane rhodopsin co-localizes with the unconventional myosin VIIa, the product of human Usher syndrome 1B gene. Furthermore, axonemal actin was identified in the photoreceptor cilium, which is spatially co-localized with myosin VIIa and opsin. This actin cytoskeleton of the cilium may provide the structural bases for myosin VIIa-linked ciliary trafficking of membrane components, including rhodopsin.
Autoren
  • U Wolfrum
  • A Schmitt
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/10891855
DOI
10.1002/1097-0169(200006)46:2<95::AID-CM2>3.0.CO;2-Q
ISSN
0886-1544
Ausgabe der Veröffentlichung
2
Zeitschrift
Cell Motil Cytoskeleton
Schlüsselwörter
  • Actins
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Biological Transport
  • Cattle
  • Cilia
  • Dyneins
  • Female
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Immunoelectron
  • Middle Aged
  • Molecular Sequence Data
  • Myosin VIIa
  • Myosins
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Rod Photoreceptor Cells
  • Retinitis Pigmentosa
  • Rhodopsin
  • Rod Cell Outer Segment
  • Rod Opsins
Sprache
eng
Country
United States
Paginierung
95 - 107
PII
10.1002/1097-0169(200006)46:2<95::AID-CM2>3.0.CO;2-Q
Datum der Veröffentlichung
2000
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2000
Titel
Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
46

Data source: PubMed

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