Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells

Publication type:
Journal article
Metadata:
Autoren
  • Markus Richard Jahn
  • Ibrahim Shukoor
  • Wolfgang Tremel
  • Uwe Wolfrum
  • Ute Kolb
  • Thomas Nawroth
  • Peter Langguth
Sammlungen
  • metadata
ISSN
0022-3573
Ausgabe der Veröffentlichung
12
Zeitschrift
Journal of pharmacy and pharmacology
Schlüsselwörter
  • 500 Naturwissenschaften
  • 500 Natural sciences and mathematics
Sprache
eng
Paginierung
Seiten: 1522 - 1530
Datum der Veröffentlichung
2011
Herausgeber
Wiley-Blackwell
Herausgeber URL
http://dx.doi.org/10.1111/j.2042-7158.2011.01356.x
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells
Ausgabe der Zeitschrift
63

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Markus Richard Jahn
  • Ibrahim Shukoor
  • Wolfgang Tremel
  • Uwe Wolfrum
  • Ute Kolb
  • Thomas Nawroth
  • Peter Langguth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000297418400003&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1111/j.2042-7158.2011.01356.x
eISSN
2042-7158
Externe Identifier
  • Clarivate Analytics Document Solution ID: 853IC
  • PubMed Identifier: 22060282
ISSN
0022-3573
Ausgabe der Veröffentlichung
12
Zeitschrift
JOURNAL OF PHARMACY AND PHARMACOLOGY
Schlüsselwörter
  • absorption
  • Caco-2 cells
  • iron deficiency
  • iron(III)-hydroxide nanoparticles
  • oral delivery
Paginierung
1522 - 1530
Datum der Veröffentlichung
2011
Status
Published
Titel
Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells
Sub types
  • Article
Ausgabe der Zeitschrift
63

Data source: Web of Science (Lite)

Abstract
<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Objectives</jats:title> <jats:p>The absorption of commonly used ferrous iron salts from intestinal segments at neutral to slightly alkaline pH is low, mainly because soluble ferrous iron is easily oxidized to poorly soluble ferric iron and ferrous iron but not ferric iron is carried by the divalent metal transporter DMT-1. Moreover, ferrous iron frequently causes gastrointestinal side effects. In iron(III)-hydroxide nanoparticles hundreds of ferric iron atoms are safely packed in nanoscaled cores surrounded by a solubilising carbohydrate shell, yet bioavailability from such particles is insufficient when compared with ferrous salts. To increase their intestinal uptake iron(III)-hydroxide nanoparticles were coupled in this study with the protoporphyrin hemin, which undergoes carrier-mediated uptake in the intestine.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Uptake of iron(III)-hydroxide nanoparticles with hemin covalently coupled by DCC reaction was measured in Caco-2 cells with a colorimetric assay and visualized by transmission electron microscopy.</jats:p> </jats:sec> <jats:sec> <jats:title>Key findings</jats:title> <jats:p>Nanoparticles were taken up by carrier-mediated transport, since uptake was temperature-dependent and increased with an increasing hemin substitution grade. Furthermore, uptake decreased with an increasing concentration of free hemin, due to competition for carrier-mediated uptake.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Hemin-coupled iron(III)-hydroxide nanoparticles were carried by a heme specific transport system, probably via receptor mediated endocytosis. It can be expected that this system shows improved absorption of iron compared with uncoupled iron(III)-hydroxide nanoparticles, which exist on the market today.</jats:p> </jats:sec>
Autoren
  • Markus Richard Jahn
  • Ibrahim Shukoor
  • Wolfgang Tremel
  • Uwe Wolfrum
  • Ute Kolb
  • Thomas Nawroth
  • Peter Langguth
DOI
10.1111/j.2042-7158.2011.01356.x
eISSN
2042-7158
ISSN
0022-3573
Ausgabe der Veröffentlichung
12
Zeitschrift
Journal of Pharmacy and Pharmacology
Sprache
en
Online publication date
2011
Paginierung
1522 - 1530
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Oxford University Press (OUP)
Herausgeber URL
http://dx.doi.org/10.1111/j.2042-7158.2011.01356.x
Datum der Datenerfassung
2021
Titel
Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells
Ausgabe der Zeitschrift
63

Data source: Crossref

Abstract
<h4>Objectives</h4>The absorption of commonly used ferrous iron salts from intestinal segments at neutral to slightly alkaline pH is low, mainly because soluble ferrous iron is easily oxidized to poorly soluble ferric iron and ferrous iron but not ferric iron is carried by the divalent metal transporter DMT-1. Moreover, ferrous iron frequently causes gastrointestinal side effects. In iron(III)-hydroxide nanoparticles hundreds of ferric iron atoms are safely packed in nanoscaled cores surrounded by a solubilising carbohydrate shell, yet bioavailability from such particles is insufficient when compared with ferrous salts. To increase their intestinal uptake iron(III)-hydroxide nanoparticles were coupled in this study with the protoporphyrin hemin, which undergoes carrier-mediated uptake in the intestine.<h4>Methods</h4>Uptake of iron(III)-hydroxide nanoparticles with hemin covalently coupled by DCC reaction was measured in Caco-2 cells with a colorimetric assay and visualized by transmission electron microscopy.<h4>Key findings</h4>Nanoparticles were taken up by carrier-mediated transport, since uptake was temperature-dependent and increased with an increasing hemin substitution grade. Furthermore, uptake decreased with an increasing concentration of free hemin, due to competition for carrier-mediated uptake.<h4>Conclusions</h4>Hemin-coupled iron(III)-hydroxide nanoparticles were carried by a heme specific transport system, probably via receptor mediated endocytosis. It can be expected that this system shows improved absorption of iron compared with uncoupled iron(III)-hydroxide nanoparticles, which exist on the market today.
Addresses
  • Biopharmacy and Pharmaceutical Technology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
Autoren
  • Markus Richard Jahn
  • Ibrahim Shukoor
  • Wolfgang Tremel
  • Uwe Wolfrum
  • Ute Kolb
  • Thomas Nawroth
  • Peter Langguth
DOI
10.1111/j.2042-7158.2011.01356.x
eISSN
2042-7158
Externe Identifier
  • PubMed Identifier: 22060282
Open access
false
ISSN
0022-3573
Ausgabe der Veröffentlichung
12
Zeitschrift
The Journal of pharmacy and pharmacology
Schlüsselwörter
  • Caco-2 Cells
  • Humans
  • Ferric Compounds
  • Ferrozine
  • Tetrazolium Salts
  • Hemin
  • Thiazoles
  • Iron Chelating Agents
  • Culture Media
  • Microscopy, Electron, Transmission
  • Spectroscopy, Fourier Transform Infrared
  • Spectrophotometry, Ultraviolet
  • Colorimetry
  • Particle Size
  • Scattering, Radiation
  • Coloring Agents
  • Nanoparticles
Sprache
eng
Medium
Print-Electronic
Online publication date
2011
Paginierung
1522 - 1530
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
63

Data source: Europe PubMed Central

Abstract
OBJECTIVES: The absorption of commonly used ferrous iron salts from intestinal segments at neutral to slightly alkaline pH is low, mainly because soluble ferrous iron is easily oxidized to poorly soluble ferric iron and ferrous iron but not ferric iron is carried by the divalent metal transporter DMT-1. Moreover, ferrous iron frequently causes gastrointestinal side effects. In iron(III)-hydroxide nanoparticles hundreds of ferric iron atoms are safely packed in nanoscaled cores surrounded by a solubilising carbohydrate shell, yet bioavailability from such particles is insufficient when compared with ferrous salts. To increase their intestinal uptake iron(III)-hydroxide nanoparticles were coupled in this study with the protoporphyrin hemin, which undergoes carrier-mediated uptake in the intestine. METHODS: Uptake of iron(III)-hydroxide nanoparticles with hemin covalently coupled by DCC reaction was measured in Caco-2 cells with a colorimetric assay and visualized by transmission electron microscopy. KEY FINDINGS: Nanoparticles were taken up by carrier-mediated transport, since uptake was temperature-dependent and increased with an increasing hemin substitution grade. Furthermore, uptake decreased with an increasing concentration of free hemin, due to competition for carrier-mediated uptake. CONCLUSIONS: Hemin-coupled iron(III)-hydroxide nanoparticles were carried by a heme specific transport system, probably via receptor mediated endocytosis. It can be expected that this system shows improved absorption of iron compared with uncoupled iron(III)-hydroxide nanoparticles, which exist on the market today.
Autoren
  • Markus Richard Jahn
  • Ibrahim Shukoor
  • Wolfgang Tremel
  • Uwe Wolfrum
  • Ute Kolb
  • Thomas Nawroth
  • Peter Langguth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/22060282
DOI
10.1111/j.2042-7158.2011.01356.x
eISSN
2042-7158
Ausgabe der Veröffentlichung
12
Zeitschrift
J Pharm Pharmacol
Schlüsselwörter
  • Caco-2 Cells
  • Colorimetry
  • Coloring Agents
  • Culture Media
  • Ferric Compounds
  • Ferrozine
  • Hemin
  • Humans
  • Iron Chelating Agents
  • Microscopy, Electron, Transmission
  • Nanoparticles
  • Particle Size
  • Scattering, Radiation
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Tetrazolium Salts
  • Thiazoles
Sprache
eng
Country
England
Paginierung
1522 - 1530
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
63

Data source: PubMed

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