Iron oxide/hydroxide nanoparticles with negatively charged shells show Increased uptake in caco-2 cells

Publication type:
Journal article
Metadata:
Autoren
  • Markus R Jahn
  • Thomas Nawroth
  • Sören Fütterer
  • Uwe Wolfrum
  • Ute Kolb
  • Peter Langguth
Sammlungen
  • metadata
ISSN
1543-8384
Ausgabe der Veröffentlichung
6
Zeitschrift
Molecular pharmaceutics
Schlüsselwörter
  • 500 Naturwissenschaften
  • 500 Natural sciences and mathematics
Sprache
eng
Paginierung
Seiten: 1628 - 1637
Datum der Veröffentlichung
2012
Herausgeber
American Chemical Society
Herausgeber URL
http://dx.doi.org/10.1021/mp200628u
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Iron oxide/hydroxide nanoparticles with negatively charged shells show Increased uptake in caco-2 cells
Ausgabe der Zeitschrift
9

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Markus R Jahn
  • Thomas Nawroth
  • Soeren Fuetterer
  • Uwe Wolfrum
  • Ute Kolb
  • Peter Langguth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000304728700010&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1021/mp200628u
Externe Identifier
  • Clarivate Analytics Document Solution ID: 951OA
  • PubMed Identifier: 22587679
ISSN
1543-8384
Ausgabe der Veröffentlichung
6
Zeitschrift
MOLECULAR PHARMACEUTICS
Schlüsselwörter
  • absorption
  • Caco-2 cells
  • iron oxide/hydroxide nanoparticles
  • iron deficiency
  • oral delivery
Paginierung
1628 - 1637
Datum der Veröffentlichung
2012
Status
Published
Titel
Iron Oxide/Hydroxide Nanoparticles with Negatively Charged Shells Show Increased Uptake in Caco-2 Cells
Sub types
  • Article
Ausgabe der Zeitschrift
9

Data source: Web of Science (Lite)

Autoren
  • Markus R Jahn
  • Thomas Nawroth
  • Sören Fütterer
  • Uwe Wolfrum
  • Ute Kolb
  • Peter Langguth
DOI
10.1021/mp200628u
eISSN
1543-8392
ISSN
1543-8384
Ausgabe der Veröffentlichung
6
Zeitschrift
Molecular Pharmaceutics
Sprache
en
Online publication date
2012
Paginierung
1628 - 1637
Datum der Veröffentlichung
2012
Status
Published
Herausgeber
American Chemical Society (ACS)
Herausgeber URL
http://dx.doi.org/10.1021/mp200628u
Datum der Datenerfassung
2023
Titel
Iron Oxide/Hydroxide Nanoparticles with Negatively Charged Shells Show Increased Uptake in Caco-2 Cells
Ausgabe der Zeitschrift
9

Data source: Crossref

Abstract
The absorption of commonly used ferrous iron salts from intestinal segments at neutral to slightly alkaline pH is low, mainly because soluble ferrous iron is easily oxidized to poorly soluble ferric iron and because ferrous iron, but not ferric iron, is carried by the divalent metal transporter DMT-1. Moreover, ferrous iron frequently causes gastrointestinal side effects. Iron hydroxide nanoparticles with neutral and hydrophilic carbohydrate shells are alternatively used to ferrous salts. In these formulations gastrointestinal side effects are rare because hundreds of ferric iron atoms are safely packed in nanoscaled cores surrounded by the solubilizing shell; nevertheless, iron bioavailability is even worse compared to ferrous salts. In this study the cell uptake of iron hydroxide and iron oxide nanoparticles (FeONP) with negatively charged shells of different chemical types and sizes was compared to the uptake of those with neutral hydrophilic shells, ferrous sulfate and ferric chloride. The nanoparticle uptake was measured in Caco-2 cells with the iron detecting ferrozine method and visualized by transmission electron microscopy. The toxicity was evaluated using the MTT assay. For nanoparticles with a negatively charged shell the iron uptake was about 40 times higher compared to those with neutral hydrophilic carbohydrate shell or ferric chloride and in the same range as ferrous sulfate. However, in contrast to ferrous sulfate, nanoparticles with negatively charged shells showed no toxicity. Two different uptake mechanisms were proposed: diffusion for hydroxide nanoparticles with neutral hydrophilic shell and adsorptive endocytosis for nanoparticles with negatively charged shells. It needs to be determined whether iron hydroxide nanoparticles with negatively charged shells also show improved bioavailability in iron-deficient patients compared to iron hydroxide nanoparticles with a neutral hydrophilic shell, which exist in the market today.
Addresses
  • Department of Biopharmacy and Pharmaceutical Technology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.
Autoren
  • Markus R Jahn
  • Thomas Nawroth
  • Sören Fütterer
  • Uwe Wolfrum
  • Ute Kolb
  • Peter Langguth
DOI
10.1021/mp200628u
eISSN
1543-8392
Externe Identifier
  • PubMed Identifier: 22587679
Open access
false
ISSN
1543-8384
Ausgabe der Veröffentlichung
6
Zeitschrift
Molecular pharmaceutics
Schlüsselwörter
  • Caco-2 Cells
  • Humans
  • Chlorides
  • Ferric Compounds
  • Microscopy, Electron, Transmission
  • Nanoparticles
Sprache
eng
Medium
Print-Electronic
Online publication date
2012
Paginierung
1628 - 1637
Datum der Veröffentlichung
2012
Status
Published
Datum der Datenerfassung
2012
Titel
Iron oxide/hydroxide nanoparticles with negatively charged shells show increased uptake in Caco-2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Data source: Europe PubMed Central

Abstract
The absorption of commonly used ferrous iron salts from intestinal segments at neutral to slightly alkaline pH is low, mainly because soluble ferrous iron is easily oxidized to poorly soluble ferric iron and because ferrous iron, but not ferric iron, is carried by the divalent metal transporter DMT-1. Moreover, ferrous iron frequently causes gastrointestinal side effects. Iron hydroxide nanoparticles with neutral and hydrophilic carbohydrate shells are alternatively used to ferrous salts. In these formulations gastrointestinal side effects are rare because hundreds of ferric iron atoms are safely packed in nanoscaled cores surrounded by the solubilizing shell; nevertheless, iron bioavailability is even worse compared to ferrous salts. In this study the cell uptake of iron hydroxide and iron oxide nanoparticles (FeONP) with negatively charged shells of different chemical types and sizes was compared to the uptake of those with neutral hydrophilic shells, ferrous sulfate and ferric chloride. The nanoparticle uptake was measured in Caco-2 cells with the iron detecting ferrozine method and visualized by transmission electron microscopy. The toxicity was evaluated using the MTT assay. For nanoparticles with a negatively charged shell the iron uptake was about 40 times higher compared to those with neutral hydrophilic carbohydrate shell or ferric chloride and in the same range as ferrous sulfate. However, in contrast to ferrous sulfate, nanoparticles with negatively charged shells showed no toxicity. Two different uptake mechanisms were proposed: diffusion for hydroxide nanoparticles with neutral hydrophilic shell and adsorptive endocytosis for nanoparticles with negatively charged shells. It needs to be determined whether iron hydroxide nanoparticles with negatively charged shells also show improved bioavailability in iron-deficient patients compared to iron hydroxide nanoparticles with a neutral hydrophilic shell, which exist in the market today.
Autoren
  • Markus R Jahn
  • Thomas Nawroth
  • Sören Fütterer
  • Uwe Wolfrum
  • Ute Kolb
  • Peter Langguth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/22587679
DOI
10.1021/mp200628u
eISSN
1543-8392
Ausgabe der Veröffentlichung
6
Zeitschrift
Mol Pharm
Schlüsselwörter
  • Caco-2 Cells
  • Chlorides
  • Ferric Compounds
  • Humans
  • Microscopy, Electron, Transmission
  • Nanoparticles
Sprache
eng
Country
United States
Paginierung
1628 - 1637
Datum der Veröffentlichung
2012
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2012
Titel
Iron oxide/hydroxide nanoparticles with negatively charged shells show increased uptake in Caco-2 cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
9

Data source: PubMed

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