Adenovirus E1A/E1B transformed amniotic fluid cells support human cytomegalovirus replication

Publication type:
Journal article
Metadata:
Autoren
  • Natascha Krömmelbein
  • Lüder Wiebusch
  • Gudrun Schiedner
  • Nicole Büscher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
Sammlungen
  • metadata
ISSN
1999-4915
Ausgabe der Veröffentlichung
2
Zeitschrift
Viruses
Schlüsselwörter
  • 610 Medizin
  • 610 Medical sciences
Sprache
eng
Paginierung
Art. 37
Datum der Veröffentlichung
2016
Herausgeber
MDPI
Herausgeber URL
http://dx.doi.org/10.3390/v8020037
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Adenovirus E1A/E1B transformed amniotic fluid cells support human cytomegalovirus replication
Ausgabe der Zeitschrift
8

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Natascha Kroemmelbein
  • Lueder Wiebusch
  • Gudrun Schiedner
  • Nicole Buescher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000371831800029&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.3390/v8020037
Externe Identifier
  • Clarivate Analytics Document Solution ID: DG1MI
  • PubMed Identifier: 26848680
ISSN
1999-4915
Ausgabe der Veröffentlichung
2
Zeitschrift
VIRUSES-BASEL
Schlüsselwörter
  • cytomegalovirus
  • amniotic fluid cells
  • adenovirus E1A
  • E1B
  • CAP
  • Cevec's aminocyte production cell line
Artikelnummer
ARTN 37
Datum der Veröffentlichung
2016
Status
Published
Titel
Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication
Sub types
  • Article
Ausgabe der Zeitschrift
8

Data source: Web of Science (Lite)

Autoren
  • Natascha Krömmelbein
  • Lüder Wiebusch
  • Gudrun Schiedner
  • Nicole Büscher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
DOI
10.3390/v8020037
eISSN
1999-4915
Ausgabe der Veröffentlichung
2
Zeitschrift
Viruses
Sprache
en
Online publication date
2016
Paginierung
37 - 37
Status
Published online
Herausgeber
MDPI AG
Herausgeber URL
http://dx.doi.org/10.3390/v8020037
Datum der Datenerfassung
2020
Titel
Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication
Ausgabe der Zeitschrift
8

Data source: Crossref

Abstract
The human cytomegalovirus (HCMV) replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection of the transforming functions of adenoviruses have been notoriously resistant to HCMV replication and progeny production. Here, we provide first-time evidence that a permanent cell line immortalized by adenovirus type 5 E1A and E1B (CAP) is supporting the full HCMV replication cycle and is releasing infectious progeny. The CAP cell line had previously been established from amniotic fluid cells which were likely derived from membranes of the developing fetus. These cells can be grown under serum-free conditions. HCMV efficiently penetrated CAP cells, expressed its immediate-early proteins and dispersed restrictive PML-bodies. Viral DNA replication was initiated and viral progeny became detectable by electron microscopy in CAP cells. Furthermore, infectious virus was released from CAP cells, yet to lower levels compared to fibroblasts. Subviral dense bodies were also secreted from CAP cells. The results show that E1A/E1B expression in transformed cells is not generally repressive to HCMV replication and that CAP cells may be a good substrate for dense body based vaccine production.
Addresses
  • Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, D-55131 Mainz, Germany. kroemme@uni-mainz.de.
Autoren
  • Natascha Krömmelbein
  • Lüder Wiebusch
  • Gudrun Schiedner
  • Nicole Büscher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
DOI
10.3390/v8020037
eISSN
1999-4915
Externe Identifier
  • PubMed Identifier: 26848680
  • PubMed Central ID: PMC4776192
Open access
true
ISSN
1999-4915
Ausgabe der Veröffentlichung
2
Zeitschrift
Viruses
Schlüsselwörter
  • Humans
  • Adenoviruses, Human
  • Cytomegalovirus
  • Adenovirus Infections, Human
  • Cytomegalovirus Infections
  • Cell Transformation, Viral
  • Adenovirus E1A Proteins
  • Adenovirus E1B Proteins
  • Virus Replication
Sprache
eng
Medium
Electronic
Online publication date
2016
Open access status
Open Access
Paginierung
E37
Datum der Veröffentlichung
2016
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2016
Titel
Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
8

Files

https://www.mdpi.com/1999-4915/8/2/37/pdf?version=1454409342 https://europepmc.org/articles/PMC4776192?pdf=render

Data source: Europe PubMed Central

Abstract
The human cytomegalovirus (HCMV) replicates to high titers in primary human fibroblast cell cultures. A variety of primary human cells and some tumor-derived cell lines do also support permissive HCMV replication, yet at low levels. Cell lines established by transfection of the transforming functions of adenoviruses have been notoriously resistant to HCMV replication and progeny production. Here, we provide first-time evidence that a permanent cell line immortalized by adenovirus type 5 E1A and E1B (CAP) is supporting the full HCMV replication cycle and is releasing infectious progeny. The CAP cell line had previously been established from amniotic fluid cells which were likely derived from membranes of the developing fetus. These cells can be grown under serum-free conditions. HCMV efficiently penetrated CAP cells, expressed its immediate-early proteins and dispersed restrictive PML-bodies. Viral DNA replication was initiated and viral progeny became detectable by electron microscopy in CAP cells. Furthermore, infectious virus was released from CAP cells, yet to lower levels compared to fibroblasts. Subviral dense bodies were also secreted from CAP cells. The results show that E1A/E1B expression in transformed cells is not generally repressive to HCMV replication and that CAP cells may be a good substrate for dense body based vaccine production.
Date of acceptance
2016
Autoren
  • Natascha Krömmelbein
  • Lüder Wiebusch
  • Gudrun Schiedner
  • Nicole Büscher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26848680
DOI
10.3390/v8020037
eISSN
1999-4915
Externe Identifier
  • PubMed Central ID: PMC4776192
Ausgabe der Veröffentlichung
2
Zeitschrift
Viruses
Schlüsselwörter
  • CAP
  • Cevec’s aminocyte production cell line
  • adenovirus E1A/E1B
  • amniotic fluid cells
  • cytomegalovirus
  • Adenovirus E1A Proteins
  • Adenovirus E1B Proteins
  • Adenovirus Infections, Human
  • Adenoviruses, Human
  • Cell Transformation, Viral
  • Cytomegalovirus
  • Cytomegalovirus Infections
  • Humans
  • Virus Replication
Sprache
eng
Country
Switzerland
PII
v8020037
Datum der Veröffentlichung
2016
Status
Published online
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Adenovirus E1A/E1B Transformed Amniotic Fluid Cells Support Human Cytomegalovirus Replication.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
8

Data source: PubMed

Author's licence
CC-BY
Autoren
  • Natascha Krömmelbein
  • Lüder Wiebusch
  • Gudrun Schiedner
  • Nicole Büscher
  • Caroline Sauer
  • Luise Florin
  • Elisabeth Sehn
  • Uwe Wolfrum
  • Bodo Plachter
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-OA-Publizieren (2012 - 2017)
Resource version
Published version
DOI
10.3390/v8020037
Funding acknowledgements
  • DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1999-4915
Ausgabe der Veröffentlichung
2
Zeitschrift
Viruses
Schlüsselwörter
  • 610 Medizin
  • 610 Medical sciences
Sprache
eng
Open access status
Open Access
Paginierung
Art. 37
Datum der Veröffentlichung
2016
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/7873
Herausgeber
MDPI
Herausgeber URL
http://dx.doi.org/10.3390/v8020037
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
Adenovirus E1A/E1B transformed amniotic fluid cells support human cytomegalovirus replication
Ausgabe der Zeitschrift
8

Files

adenovirus_e1ae1b_transformed-20220914234420920.pdf

Data source: OPENSCIENCE.UB

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