Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function

Publication type:
Journal article
Metadata:
Autoren
  • Mark Helm
  • Andrei Yu Kobitski
  • G Ulrich Nienhaus
DOI
10.1007/s12551-009-0018-3
eISSN
1867-2469
ISSN
1867-2450
Ausgabe der Veröffentlichung
4
Zeitschrift
Biophysical Reviews
Sprache
en
Online publication date
2009
Paginierung
161 - 176
Datum der Veröffentlichung
2009
Status
Published
Herausgeber
Springer Science and Business Media LLC
Herausgeber URL
http://dx.doi.org/10.1007/s12551-009-0018-3
Datum der Datenerfassung
2019
Titel
Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function
Ausgabe der Zeitschrift
1

Data source: Crossref

Other metadata sources:
Abstract
Single-molecule fluorescence microscopy experiments on RNA molecules brought to light the highly complex dynamics of key biological processes, including RNA folding, catalysis of ribozymes, ligand sensing of riboswitches and aptamers, and protein synthesis in the ribosome. By using highly advanced biophysical spectroscopy techniques in combination with sophisticated biochemical synthesis approaches, molecular dynamics of individual RNA molecules can be observed in real time and under physiological conditions in unprecedented detail that cannot be achieved with bulk experiments. Here, we review recent advances in RNA folding and functional studies of RNA and RNA-protein complexes addressed by using single-molecule Förster (fluorescence) resonance energy transfer (smFRET) technique.
Addresses
  • Institute of Pharmacy, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany. mhelm@uni-mainz.de.
Autoren
  • Mark Helm
  • Andrei Yu Kobitski
  • G Ulrich Nienhaus
DOI
10.1007/s12551-009-0018-3
eISSN
1867-2469
Externe Identifier
  • PubMed Identifier: 28510027
  • PubMed Central ID: PMC5418384
Open access
false
ISSN
1867-2450
Ausgabe der Veröffentlichung
4
Zeitschrift
Biophysical reviews
Sprache
eng
Medium
Print-Electronic
Online publication date
2009
Paginierung
161
Datum der Veröffentlichung
2009
Status
Published
Datum der Datenerfassung
2017
Titel
Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function.
Sub types
  • review-article
  • Review
  • Journal Article
Ausgabe der Zeitschrift
1

Data source: Europe PubMed Central

Abstract
Single-molecule fluorescence microscopy experiments on RNA molecules brought to light the highly complex dynamics of key biological processes, including RNA folding, catalysis of ribozymes, ligand sensing of riboswitches and aptamers, and protein synthesis in the ribosome. By using highly advanced biophysical spectroscopy techniques in combination with sophisticated biochemical synthesis approaches, molecular dynamics of individual RNA molecules can be observed in real time and under physiological conditions in unprecedented detail that cannot be achieved with bulk experiments. Here, we review recent advances in RNA folding and functional studies of RNA and RNA-protein complexes addressed by using single-molecule Förster (fluorescence) resonance energy transfer (smFRET) technique.
Date of acceptance
2009
Autoren
  • Mark Helm
  • Andrei Yu Kobitski
  • G Ulrich Nienhaus
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/28510027
DOI
10.1007/s12551-009-0018-3
Externe Identifier
  • PubMed Central ID: PMC5418384
ISSN
1867-2450
Ausgabe der Veröffentlichung
4
Zeitschrift
Biophys Rev
Schlüsselwörter
  • RNA
  • RNA folding
  • RNA-protein complexes
  • Single-molecule Förster resonance energy transfer technique
Sprache
eng
Country
Germany
Paginierung
161
PII
10.1007/s12551-009-0018-3
Datum der Veröffentlichung
2009
Status
Published
Titel
Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function.
Sub types
  • Journal Article
  • Review
Ausgabe der Zeitschrift
1

Data source: PubMed

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