FRET Imaging of Cells Transfected with siRNA/Liposome Complexes

Publication type:
Journal article
Metadata:
Autoren
  • Il-Han Kim
  • Anne Järve
  • Markus Hirsch
  • Roger Fischer
  • Michael F Trendelenburg
  • Ulrich Massing
  • Karl Rohr
  • Mark Helm
DOI
10.1007/978-1-60761-447-0_30
eISSN
1940-6029
ISSN
1064-3745
Online publication date
2009
Paginierung
439 - 455
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Humana Press
Herausgeber URL
http://dx.doi.org/10.1007/978-1-60761-447-0_30
Datum der Datenerfassung
2021
Titel
FRET Imaging of Cells Transfected with siRNA/Liposome Complexes

Data source: Crossref

Other metadata sources:
Abstract
By monitoring the efficiency of fluorescence resonance energy transfer of dyes attached to the different strands of siRNA, the structural integrity of the latter can be traced inside cells. Here, the experimental details of dye-labeled siRNA construction, tissue culture, and transfection with liposomally formulated siRNAs are given, as well as the conditions for confocal microscopy and an algorithm allowing the visualization of intact siRNA after image data treatment. The method allows rapid screening of different liposomal siRNA formulations, obtained by small scale dual asymmetric centrifugation with high entrapping efficiency.
Addresses
  • Department of Bioinformatics and Functional Genomics, German Cancer Research Center, Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Germany.
Autoren
  • Il-Han Kim
  • Anne Järve
  • Markus Hirsch
  • Roger Fischer
  • Michael F Trendelenburg
  • Ulrich Massing
  • Karl Rohr
  • Mark Helm
DOI
10.1007/978-1-60761-447-0_30
eISSN
1940-6029
Externe Identifier
  • PubMed Identifier: 20013414
Open access
false
ISSN
1064-3745
Zeitschrift
Methods in molecular biology (Clifton, N.J.)
Schlüsselwörter
  • Cell Line
  • Endothelial Cells
  • Animals
  • Humans
  • Rats
  • RNA, Small Interfering
  • Liposomes
  • Fluorescent Dyes
  • Fluorescence Resonance Energy Transfer
  • Tissue Fixation
  • Microinjections
  • Transfection
Sprache
eng
Medium
Print
Paginierung
439 - 455
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2009
Titel
FRET imaging of cells transfected with siRNA/liposome complexes.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
606

Data source: Europe PubMed Central

Abstract
By monitoring the efficiency of fluorescence resonance energy transfer of dyes attached to the different strands of siRNA, the structural integrity of the latter can be traced inside cells. Here, the experimental details of dye-labeled siRNA construction, tissue culture, and transfection with liposomally formulated siRNAs are given, as well as the conditions for confocal microscopy and an algorithm allowing the visualization of intact siRNA after image data treatment. The method allows rapid screening of different liposomal siRNA formulations, obtained by small scale dual asymmetric centrifugation with high entrapping efficiency.
Autoren
  • Il-Han Kim
  • Anne Järve
  • Markus Hirsch
  • Roger Fischer
  • Michael F Trendelenburg
  • Ulrich Massing
  • Karl Rohr
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20013414
DOI
10.1007/978-1-60761-447-0_30
eISSN
1940-6029
Zeitschrift
Methods Mol Biol
Schlüsselwörter
  • Animals
  • Cell Line
  • Endothelial Cells
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes
  • Humans
  • Liposomes
  • Microinjections
  • RNA, Small Interfering
  • Rats
  • Tissue Fixation
  • Transfection
Sprache
eng
Country
United States
Paginierung
439 - 455
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
FRET imaging of cells transfected with siRNA/liposome complexes.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
606

Data source: PubMed

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