RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage

Publication type:
Journal article
Metadata:
Autoren
  • Matthias Schaefer
  • Tim Pollex
  • Katharina Hanna
  • Francesca Tuorto
  • Madeleine Meusburger
  • Mark Helm
  • Frank Lyko
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000280597200004&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1101/gad.586710
eISSN
1549-5477
Externe Identifier
  • Clarivate Analytics Document Solution ID: 634QE
  • PubMed Identifier: 20679393
ISSN
0890-9369
Ausgabe der Veröffentlichung
15
Zeitschrift
GENES & DEVELOPMENT
Schlüsselwörter
  • Cytosine methylation
  • Dnmt2
  • tRNA
  • angiogenin
Paginierung
1590 - 1595
Datum der Veröffentlichung
2010
Status
Published
Titel
RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
Sub types
  • Article
Ausgabe der Zeitschrift
24

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:p>Dnmt2 proteins are the most conserved members of the DNA methyltransferase enzyme family, but their substrate specificity and biological functions have been a subject of controversy. We show here that, in addition to tRNA<jats:sup>Asp-GTC</jats:sup>, tRNA<jats:sup>Val-AAC</jats:sup> and tRNA<jats:sup>Gly-GCC</jats:sup> are also methylated by Dnmt2. <jats:italic>Drosophila</jats:italic> Dnmt2 mutants showed reduced viability under stress conditions, and Dnmt2 relocalized to stress granules following heat shock. Strikingly, stress-induced cleavage of tRNAs was Dnmt2-dependent, and Dnmt2-mediated methylation protected tRNAs against ribonuclease cleavage. These results uncover a novel biological function of Dnmt2-mediated tRNA methylation, and suggest a role for Dnmt2 enzymes during the biogenesis of tRNA-derived small RNAs.</jats:p>
Autoren
  • Matthias Schaefer
  • Tim Pollex
  • Katharina Hanna
  • Francesca Tuorto
  • Madeleine Meusburger
  • Mark Helm
  • Frank Lyko
DOI
10.1101/gad.586710
eISSN
1549-5477
ISSN
0890-9369
Ausgabe der Veröffentlichung
15
Zeitschrift
Genes &amp; Development
Sprache
en
Online publication date
2010
Paginierung
1590 - 1595
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Cold Spring Harbor Laboratory
Herausgeber URL
http://dx.doi.org/10.1101/gad.586710
Datum der Datenerfassung
2021
Titel
RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage
Ausgabe der Zeitschrift
24

Data source: Crossref

Abstract
Dnmt2 proteins are the most conserved members of the DNA methyltransferase enzyme family, but their substrate specificity and biological functions have been a subject of controversy. We show here that, in addition to tRNA(Asp-GTC), tRNA(Val-AAC) and tRNA(Gly-GCC) are also methylated by Dnmt2. Drosophila Dnmt2 mutants showed reduced viability under stress conditions, and Dnmt2 relocalized to stress granules following heat shock. Strikingly, stress-induced cleavage of tRNAs was Dnmt2-dependent, and Dnmt2-mediated methylation protected tRNAs against ribonuclease cleavage. These results uncover a novel biological function of Dnmt2-mediated tRNA methylation, and suggest a role for Dnmt2 enzymes during the biogenesis of tRNA-derived small RNAs.
Addresses
  • Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg 69120, Germany.
Autoren
  • Matthias Schaefer
  • Tim Pollex
  • Katharina Hanna
  • Francesca Tuorto
  • Madeleine Meusburger
  • Mark Helm
  • Frank Lyko
DOI
10.1101/gad.586710
eISSN
1549-5477
Externe Identifier
  • PubMed Identifier: 20679393
  • PubMed Central ID: PMC2912555
Open access
false
ISSN
0890-9369
Ausgabe der Veröffentlichung
15
Zeitschrift
Genes & development
Schlüsselwörter
  • Animals
  • Mice
  • Drosophila melanogaster
  • Ribonuclease, Pancreatic
  • Drosophila Proteins
  • RNA
  • RNA, Transfer
  • Survival Analysis
  • Sequence Deletion
  • Methylation
  • Female
  • Male
  • Stress, Physiological
  • DNA (Cytosine-5-)-Methyltransferases
Sprache
eng
Medium
Print
Paginierung
1590 - 1595
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
24

Files

http://genesdev.cshlp.org/content/24/15/1590.full.pdf http://www.genesdev.org/cgi/reprint/24/15/1590 https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20679393/pdf/?tool=EBI https://europepmc.org/articles/PMC2912555?pdf=render

Data source: Europe PubMed Central

Abstract
Dnmt2 proteins are the most conserved members of the DNA methyltransferase enzyme family, but their substrate specificity and biological functions have been a subject of controversy. We show here that, in addition to tRNA(Asp-GTC), tRNA(Val-AAC) and tRNA(Gly-GCC) are also methylated by Dnmt2. Drosophila Dnmt2 mutants showed reduced viability under stress conditions, and Dnmt2 relocalized to stress granules following heat shock. Strikingly, stress-induced cleavage of tRNAs was Dnmt2-dependent, and Dnmt2-mediated methylation protected tRNAs against ribonuclease cleavage. These results uncover a novel biological function of Dnmt2-mediated tRNA methylation, and suggest a role for Dnmt2 enzymes during the biogenesis of tRNA-derived small RNAs.
Autoren
  • Matthias Schaefer
  • Tim Pollex
  • Katharina Hanna
  • Francesca Tuorto
  • Madeleine Meusburger
  • Mark Helm
  • Frank Lyko
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20679393
DOI
10.1101/gad.586710
eISSN
1549-5477
Externe Identifier
  • PubMed Central ID: PMC2912555
Ausgabe der Veröffentlichung
15
Zeitschrift
Genes Dev
Schlüsselwörter
  • Animals
  • DNA (Cytosine-5-)-Methyltransferases
  • Drosophila Proteins
  • Drosophila melanogaster
  • Female
  • Male
  • Methylation
  • Mice
  • RNA
  • RNA, Transfer
  • Ribonuclease, Pancreatic
  • Sequence Deletion
  • Stress, Physiological
  • Survival Analysis
Sprache
eng
Country
United States
Paginierung
1590 - 1595
PII
24/15/1590
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
24

Data source: PubMed

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