Single-Molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial tRNALys

Publication type:
Journal article
Metadata:
Autoren
  • Andrei Yu Kobitski
  • Martin Hengesbach
  • Salifu Seidu-Larry
  • Kirsten Dammertz
  • Christine S Chow
  • Arthur van Aerschot
  • G Ulrich Nienhaus
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000294085200015&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.chembiol.2011.03.016
eISSN
1879-1301
Externe Identifier
  • Clarivate Analytics Document Solution ID: 809VQ
  • PubMed Identifier: 21802013
ISSN
1074-5521
Ausgabe der Veröffentlichung
7
Zeitschrift
CHEMISTRY & BIOLOGY
Paginierung
928 - 936
Datum der Veröffentlichung
2011
Status
Published
Titel
Single-Molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial tRNA<SUP>Lys</SUP>
Sub types
  • Article
Ausgabe der Zeitschrift
18

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Andrei Yu Kobitski
  • Martin Hengesbach
  • Salifu Seidu-Larry
  • Kirsten Dammertz
  • Christine S Chow
  • Arthur van Aerschot
  • G Ulrich Nienhaus
  • Mark Helm
DOI
10.1016/j.chembiol.2011.03.016
ISSN
1074-5521
Ausgabe der Veröffentlichung
7
Zeitschrift
Chemistry &amp; Biology
Sprache
en
Paginierung
928 - 936
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.chembiol.2011.03.016
Datum der Datenerfassung
2023
Titel
Single-Molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial tRNALys
Ausgabe der Zeitschrift
18

Data source: Crossref

Abstract
Using a combination of advanced RNA synthesis techniques and single molecule spectroscopy, the deconvolution of individual contributions of posttranscriptional modifications to the overall folding and stabilization of human mitochondrial tRNA(Lys) is described. An unexpected destabilizing effect of two pseudouridines on the native tRNA folding was evidenced. Furthermore, the presence of m(2)G10 alone does not facilitate the folding of tRNA(Lys), but a stabilization of the biologically functional cloverleaf shape in conjunction with the principal stabilizing component m(1)A9 exceeds the contribution of m(1)A alone. This constitutes an unprecedented cooperative effect of two nucleotide modifications in the context of a naturally occurring RNA, which may be of general importance for tRNA structure and help understanding several recently described decay pathways for hypomodified tRNAs.
Addresses
  • Institute of Applied Physics and Center for Functional Nanostructures, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
Autoren
  • Andrei Yu Kobitski
  • Martin Hengesbach
  • Salifu Seidu-Larry
  • Kirsten Dammertz
  • Christine S Chow
  • Arthur van Aerschot
  • G Ulrich Nienhaus
  • Mark Helm
DOI
10.1016/j.chembiol.2011.03.016
eISSN
1879-1301
Externe Identifier
  • PubMed Identifier: 21802013
Open access
false
ISSN
1074-5521
Ausgabe der Veröffentlichung
7
Zeitschrift
Chemistry & biology
Schlüsselwörter
  • Mitochondria
  • Humans
  • Magnesium
  • Organophosphorus Compounds
  • RNA, Transfer, Lys
  • Pseudouridine
  • Fluorescence Resonance Energy Transfer
  • Base Sequence
  • Nucleic Acid Conformation
  • RNA Stability
  • Models, Molecular
  • Molecular Sequence Data
  • Coloring Agents
Sprache
eng
Medium
Print
Paginierung
928 - 936
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Single-molecule FRET reveals a cooperative effect of two methyl group modifications in the folding of human mitochondrial tRNA(Lys).
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
18

Data source: Europe PubMed Central

Abstract
Using a combination of advanced RNA synthesis techniques and single molecule spectroscopy, the deconvolution of individual contributions of posttranscriptional modifications to the overall folding and stabilization of human mitochondrial tRNA(Lys) is described. An unexpected destabilizing effect of two pseudouridines on the native tRNA folding was evidenced. Furthermore, the presence of m(2)G10 alone does not facilitate the folding of tRNA(Lys), but a stabilization of the biologically functional cloverleaf shape in conjunction with the principal stabilizing component m(1)A9 exceeds the contribution of m(1)A alone. This constitutes an unprecedented cooperative effect of two nucleotide modifications in the context of a naturally occurring RNA, which may be of general importance for tRNA structure and help understanding several recently described decay pathways for hypomodified tRNAs.
Date of acceptance
2011
Autoren
  • Andrei Yu Kobitski
  • Martin Hengesbach
  • Salifu Seidu-Larry
  • Kirsten Dammertz
  • Christine S Chow
  • Arthur van Aerschot
  • G Ulrich Nienhaus
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21802013
DOI
10.1016/j.chembiol.2011.03.016
eISSN
1879-1301
Ausgabe der Veröffentlichung
7
Zeitschrift
Chem Biol
Schlüsselwörter
  • Base Sequence
  • Coloring Agents
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Magnesium
  • Mitochondria
  • Models, Molecular
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Organophosphorus Compounds
  • Pseudouridine
  • RNA Stability
  • RNA, Transfer, Lys
Sprache
eng
Country
United States
Paginierung
928 - 936
PII
S1074-5521(11)00173-6
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
Single-molecule FRET reveals a cooperative effect of two methyl group modifications in the folding of human mitochondrial tRNA(Lys).
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
18

Data source: PubMed

Beziehungen:
Property of

Tools