High-throughput sequencing for 1-methyladenosine (m1A) mapping in RNA
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Lyudmil Tserovski
- Virginie Marchand
- Ralf Hauenschild
- Florence Blanloeil-Oillo
- Mark Helm
- Yuri Motorin
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000383733500014&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.ymeth.2016.02.012
- eISSN
- 1095-9130
- Externe Identifier
- Clarivate Analytics Document Solution ID: DW6CH
- PubMed Identifier: 26922842
- ISSN
- 1046-2023
- Zeitschrift
- METHODS
- Schlüsselwörter
- RNA modification
- High-throughput sequencing
- Misincorporation signature
- Reverse transcription
- Paginierung
- 110 - 121
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Titel
- High-throughput sequencing for 1-methyladenosine (m<SUP>1</SUP>A) mapping in RNA
- Sub types
- Article
- Ausgabe der Zeitschrift
- 107
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Lyudmil Tserovski
- Virginie Marchand
- Ralf Hauenschild
- Florence Blanloeil-Oillo
- Mark Helm
- Yuri Motorin
- DOI
- 10.1016/j.ymeth.2016.02.012
- ISSN
- 1046-2023
- Zeitschrift
- Methods
- Sprache
- en
- Paginierung
- 110 - 121
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.ymeth.2016.02.012
- Datum der Datenerfassung
- 2019
- Titel
- High-throughput sequencing for 1-methyladenosine (m1A) mapping in RNA
- Ausgabe der Zeitschrift
- 107
Data source: Crossref
- Abstract
- Detection and mapping of modified nucleotides in RNAs is a difficult and laborious task. Several physico-chemical approaches based on differential properties of modified nucleotides can be used, however, most of these methods do not allow high-throughput analysis. Here we describe in details a method for mapping of rather common 1-methyladenosine (m(1)A) residues using high-throughput next generation sequencing (NGS). Since m(1)A residues block primer extension during reverse transcription (RT), the accumulation of abortive products as well as the nucleotide misincorporation can be detected in the sequencing data. The described library preparation protocol allows to capture both types of cDNA products essential for further bioinformatic analysis. We demonstrate that m(1)A residues produce characteristic arrest and mismatch rates and combination of both can be used for their detection as well as for discrimination of m(1)A from other modified A residues present in RNAs.
- Addresses
- Institute of Pharmacy and Biochemistry, Johannes Gutenberg University Mainz, Staudingerweg 5, 55128 Mainz, Germany.
- Autoren
- Lyudmil Tserovski
- Virginie Marchand
- Ralf Hauenschild
- Florence Blanloeil-Oillo
- Mark Helm
- Yuri Motorin
- DOI
- 10.1016/j.ymeth.2016.02.012
- eISSN
- 1095-9130
- Externe Identifier
- PubMed Identifier: 26922842
- Open access
- false
- ISSN
- 1046-2023
- Zeitschrift
- Methods (San Diego, Calif.)
- Schlüsselwörter
- RNA
- Adenosine
- RNA Processing, Post-Transcriptional
- Gene Library
- High-Throughput Nucleotide Sequencing
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2016
- Paginierung
- 110 - 121
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum der Datenerfassung
- 2016
- Titel
- High-throughput sequencing for 1-methyladenosine (m(1)A) mapping in RNA.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 107
Data source: Europe PubMed Central
- Abstract
- Detection and mapping of modified nucleotides in RNAs is a difficult and laborious task. Several physico-chemical approaches based on differential properties of modified nucleotides can be used, however, most of these methods do not allow high-throughput analysis. Here we describe in details a method for mapping of rather common 1-methyladenosine (m(1)A) residues using high-throughput next generation sequencing (NGS). Since m(1)A residues block primer extension during reverse transcription (RT), the accumulation of abortive products as well as the nucleotide misincorporation can be detected in the sequencing data. The described library preparation protocol allows to capture both types of cDNA products essential for further bioinformatic analysis. We demonstrate that m(1)A residues produce characteristic arrest and mismatch rates and combination of both can be used for their detection as well as for discrimination of m(1)A from other modified A residues present in RNAs.
- Date of acceptance
- 2016
- Autoren
- Lyudmil Tserovski
- Virginie Marchand
- Ralf Hauenschild
- Florence Blanloeil-Oillo
- Mark Helm
- Yuri Motorin
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/26922842
- DOI
- 10.1016/j.ymeth.2016.02.012
- eISSN
- 1095-9130
- Zeitschrift
- Methods
- Schlüsselwörter
- High-throughput sequencing
- Misincorporation signature
- RNA modification
- Reverse transcription
- Adenosine
- Gene Library
- High-Throughput Nucleotide Sequencing
- RNA
- RNA Processing, Post-Transcriptional
- Sprache
- eng
- Country
- United States
- Paginierung
- 110 - 121
- PII
- S1046-2023(16)30029-9
- Datum der Veröffentlichung
- 2016
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2018
- Titel
- High-throughput sequencing for 1-methyladenosine (m(1)A) mapping in RNA.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 107
Data source: PubMed
- Beziehungen:
- Property of