Recognition of Specified RNA Modifications by the Innate Immune System

Publication type:
Journal article
Metadata:
Autoren
  • Tatjana Eigenbrod
  • Patrick Keller
  • Steffen Kaiser
  • Katharina Rimbach
  • Alexander H Dalpke
  • Mark Helm
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000376194300005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/bs.mie.2015.03.006
Externe Identifier
  • Clarivate Analytics Document Solution ID: BE8BH
  • PubMed Identifier: 26253966
ISSN
0076-6879
Zeitschrift
RNA MODIFICATION
Paginierung
73 - 89
Datum der Veröffentlichung
2015
Status
Published
Titel
Recognition of Specified RNA Modifications by the Innate Immune System
Sub types
  • Review
  • Book Chapter
Ausgabe der Zeitschrift
560

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Tatjana Eigenbrod
  • Patrick Keller
  • Steffen Kaiser
  • Katharina Rimbach
  • Alexander H Dalpke
  • Mark Helm
DOI
10.1016/bs.mie.2015.03.006
ISSN
0076-6879
Paginierung
73 - 89
Datum der Veröffentlichung
2015
Status
Published
Herausgeber
Elsevier
Herausgeber URL
http://dx.doi.org/10.1016/bs.mie.2015.03.006
Datum der Datenerfassung
2018
Titel
Recognition of Specified RNA Modifications by the Innate Immune System

Data source: Crossref

Abstract
Microbial nucleic acids have been described as important activators of human innate immune responses by triggering so-called pattern recognition receptors (PRRs) that are expressed on innate immune cells, including plasmacytoid dendritic cells and monocytes. Although host and microbial nucleic acids share pronounced chemical and structural similarities, they significantly differ in their posttranscriptional modification profile, allowing the host to discriminate between self and nonself. In this regard, ribose 2'-O-methylation has been discovered as suppressor of RNA-induced PRR activation. Although 2'-O-methylation occurs with higher frequencies in eukaryotic than in prokaryotic RNA, the immunosuppressive properties of 2'-O-methylated nucleotides may be misused by certain bacteria as immune evasion mechanism. In the course of identifying inhibitory RNA modifications, our groups have synthesized and comparatively analyzed a series of differentially modified RNAs, so-called modivariants, for their immune stimulatory capacities. In this chapter, we will detail the protocols for the design and synthesis of RNA modivariants by molecular cut-and-paste techniques (referred to as molecular surgery) and describe testing of their immune stimulatory properties upon transfection into peripheral blood mononuclear cells.
Addresses
  • Department of Infectious Diseases, Medical Microbiology and Hygiene, University of Heidelberg, Heidelberg, Germany.
Autoren
  • Tatjana Eigenbrod
  • Patrick Keller
  • Steffen Kaiser
  • Katharina Rimbach
  • Alexander H Dalpke
  • Mark Helm
DOI
10.1016/bs.mie.2015.03.006
eISSN
1557-7988
Externe Identifier
  • PubMed Identifier: 26253966
Open access
false
ISSN
0076-6879
Zeitschrift
Methods in enzymology
Schlüsselwörter
  • Dendritic Cells
  • Leukocytes, Mononuclear
  • Monocytes
  • Humans
  • Ribose
  • Nucleic Acids
  • RNA
  • RNA, Bacterial
  • RNA Processing, Post-Transcriptional
  • Methylation
  • Receptors, Pattern Recognition
  • Immunity, Innate
Sprache
eng
Medium
Print-Electronic
Online publication date
2015
Paginierung
73 - 89
Datum der Veröffentlichung
2015
Status
Published
Datum der Datenerfassung
2015
Titel
Recognition of Specified RNA Modifications by the Innate Immune System.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
560

Data source: Europe PubMed Central

Abstract
Microbial nucleic acids have been described as important activators of human innate immune responses by triggering so-called pattern recognition receptors (PRRs) that are expressed on innate immune cells, including plasmacytoid dendritic cells and monocytes. Although host and microbial nucleic acids share pronounced chemical and structural similarities, they significantly differ in their posttranscriptional modification profile, allowing the host to discriminate between self and nonself. In this regard, ribose 2'-O-methylation has been discovered as suppressor of RNA-induced PRR activation. Although 2'-O-methylation occurs with higher frequencies in eukaryotic than in prokaryotic RNA, the immunosuppressive properties of 2'-O-methylated nucleotides may be misused by certain bacteria as immune evasion mechanism. In the course of identifying inhibitory RNA modifications, our groups have synthesized and comparatively analyzed a series of differentially modified RNAs, so-called modivariants, for their immune stimulatory capacities. In this chapter, we will detail the protocols for the design and synthesis of RNA modivariants by molecular cut-and-paste techniques (referred to as molecular surgery) and describe testing of their immune stimulatory properties upon transfection into peripheral blood mononuclear cells.
Autoren
  • Tatjana Eigenbrod
  • Patrick Keller
  • Steffen Kaiser
  • Katharina Rimbach
  • Alexander H Dalpke
  • Mark Helm
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26253966
DOI
10.1016/bs.mie.2015.03.006
eISSN
1557-7988
Zeitschrift
Methods Enzymol
Schlüsselwörter
  • Catalytic DNA
  • DNA splint
  • DNAzyme
  • Immune stimulation
  • Ligation
  • Modivariant
  • Molecular surgery
  • Plasmacytoid dendritic cells
  • RNA cleavage
  • tRNA
  • Dendritic Cells
  • Humans
  • Immunity, Innate
  • Leukocytes, Mononuclear
  • Methylation
  • Monocytes
  • Nucleic Acids
  • RNA
  • RNA Processing, Post-Transcriptional
  • RNA, Bacterial
  • Receptors, Pattern Recognition
  • Ribose
Sprache
eng
Country
United States
Paginierung
73 - 89
PII
S0076-6879(15)00232-3
Datum der Veröffentlichung
2015
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Recognition of Specified RNA Modifications by the Innate Immune System.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
560

Data source: PubMed

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