Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Yuri Motorin
- Mark Helm
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000459743800035&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/genes10010035
- eISSN
- 2073-4425
- Externe Identifier
- Clarivate Analytics Document Solution ID: HM8PR
- PubMed Identifier: 30634534
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- GENES
- Schlüsselwörter
- RNA modification
- epitranscriptome
- deep sequencing
- Next Generation Sequencing
- RNA-Seq
- chemical treatment
- engineered Reverse Transcriptase enzymes
- Artikelnummer
- ARTN 35
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
- Sub types
- Review
- Ausgabe der Zeitschrift
- 10
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>New analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification mapping. In this process, positions inside a cell`s transcriptome are receiving a status of potential modification sites (so called modification calling), typically based on a score of some kind that issues from the particular method applied. The resulting data are thought to represent information that goes beyond what is contained in typical transcriptome data, and hence the field has taken to use the term “epitranscriptome”. Due to the high rate of newly published mapping techniques, a significant number of chemically distinct RNA modifications have become amenable to mapping, albeit with variegated accuracy and precision, depending on the nature of the technique. This review gives a brief overview of known techniques, and how they were applied to modification calling.</jats:p>
- Autoren
- Yuri Motorin
- Mark Helm
- DOI
- 10.3390/genes10010035
- eISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Genes
- Sprache
- en
- Online publication date
- 2019
- Paginierung
- 35 - 35
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/genes10010035
- Datum der Datenerfassung
- 2020
- Titel
- Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
- Ausgabe der Zeitschrift
- 10
Data source: Crossref
- Abstract
- New analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification mapping. In this process, positions inside a cell`s transcriptome are receiving a status of potential modification sites (so called modification calling), typically based on a score of some kind that issues from the particular method applied. The resulting data are thought to represent information that goes beyond what is contained in typical transcriptome data, and hence the field has taken to use the term "epitranscriptome". Due to the high rate of newly published mapping techniques, a significant number of chemically distinct RNA modifications have become amenable to mapping, albeit with variegated accuracy and precision, depending on the nature of the technique. This review gives a brief overview of known techniques, and how they were applied to modification calling.
- Addresses
- Laboratoire IMoPA, UMR7365 National Centre for Scientific Research (CNRS)-Lorraine University, Biopôle, 9 Avenue de la Forêt de Haye, 54505 Vandoeuvre-les-Nancy, France. Yuri.Motorin@univ-lorraine.fr.
- Autoren
- Yuri Motorin
- Mark Helm
- DOI
- 10.3390/genes10010035
- eISSN
- 2073-4425
- Externe Identifier
- PubMed Identifier: 30634534
- PubMed Central ID: PMC6356707
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SPP1784
- Deutsche Forschungsgemeinschaft: HE3397/13-2
- Deutsche Forschungsgemeinschaft: HE3397/8-1
- Agence Nationale de la Recherche: HTRNA-Mod ANR-13-ISV8-0001
- Conseil Régional de Lorraine: Méthylation aberrante des ARN
- Open access
- true
- ISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Genes
- Schlüsselwörter
- Animals
- Humans
- RNA
- Sequence Analysis, RNA
- RNA Processing, Post-Transcriptional
- High-Throughput Nucleotide Sequencing
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- E35
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies.
- Sub types
- Research Support, Non-U.S. Gov't
- review-article
- Review
- Journal Article
- Ausgabe der Zeitschrift
- 10
Files
https://www.mdpi.com/2073-4425/10/1/35/pdf?version=1547199550 https://europepmc.org/articles/PMC6356707?pdf=render
Data source: Europe PubMed Central
- Abstract
- New analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification mapping. In this process, positions inside a cell`s transcriptome are receiving a status of potential modification sites (so called modification calling), typically based on a score of some kind that issues from the particular method applied. The resulting data are thought to represent information that goes beyond what is contained in typical transcriptome data, and hence the field has taken to use the term "epitranscriptome". Due to the high rate of newly published mapping techniques, a significant number of chemically distinct RNA modifications have become amenable to mapping, albeit with variegated accuracy and precision, depending on the nature of the technique. This review gives a brief overview of known techniques, and how they were applied to modification calling.
- Date of acceptance
- 2019
- Autoren
- Yuri Motorin
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/30634534
- DOI
- 10.3390/genes10010035
- Externe Identifier
- PubMed Central ID: PMC6356707
- ISSN
- 2073-4425
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Genes (Basel)
- Schlüsselwörter
- Next Generation Sequencing
- RNA modification
- RNA-Seq
- chemical treatment
- deep sequencing
- engineered Reverse Transcriptase enzymes
- epitranscriptome
- Animals
- High-Throughput Nucleotide Sequencing
- Humans
- RNA
- RNA Processing, Post-Transcriptional
- Sequence Analysis, RNA
- Sprache
- eng
- Country
- Switzerland
- PII
- genes10010035
- Datum der Veröffentlichung
- 2019
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2019
- Titel
- Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Review
- Ausgabe der Zeitschrift
- 10
Data source: PubMed
- Beziehungen:
- Property of