Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Claudia Weber
- Matthias Voigt
- Johanna Simon
- Ann-Kathrin Danner
- Holger Frey
- Volker Mailaender
- Mark Helm
- Svenja Morsbach
- Katharina Landfester
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000480826700010&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/acs.biomac.9b00539
- eISSN
- 1526-4602
- Externe Identifier
- Clarivate Analytics Document Solution ID: IQ5XU
- PubMed Identifier: 31268685
- ISSN
- 1525-7797
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- BIOMACROMOLECULES
- Paginierung
- 2989 - 2999
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona
- Sub types
- Article
- Ausgabe der Zeitschrift
- 20
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Claudia Weber
- Matthias Voigt
- Johanna Simon
- Ann-Kathrin Danner
- Holger Frey
- Volker Mailänder
- Mark Helm
- Svenja Morsbach
- Katharina Landfester
- DOI
- 10.1021/acs.biomac.9b00539
- eISSN
- 1526-4602
- ISSN
- 1525-7797
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Biomacromolecules
- Sprache
- en
- Online publication date
- 2019
- Paginierung
- 2989 - 2999
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/acs.biomac.9b00539
- Datum der Datenerfassung
- 2023
- Titel
- Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona
- Ausgabe der Zeitschrift
- 20
Data source: Crossref
- Abstract
- Liposomes are established drug carriers that are employed to transport and deliver hydrophilic drugs in the body. To minimize unspecific cellular uptake, nanocarriers are commonly modified with poly(ethylene glycol) (PEG), which is known to minimize unspecific protein adsorption. However, to date, it has not been studied whether this is an intrinsic and specific property of PEG or if it can be transferred to hyperbranched polyglycerol (<i>hb</i>PG) as well. Additionally, it remains unclear if the reduction of unspecific cell uptake is independent of the "basic" carrier at which a surface functionalization with polymers is usually applied. Therefore, we studied the protein corona of differently functionalized liposomes (unfunctionalized vs PEG or <i>hb</i>PG-functionalized) using PEGylated and PGylated lipids. Their cellular uptake in macrophages was compared. For all three liposomal samples, rather similar protein corona compositions were found, and also-more importantly-the total amount of proteins adsorbed was very low compared to other nanoparticles. Interestingly, the cellular uptake was then significantly changed by the surface functionalization itself, despite the adsorption of a small amount of proteins: although the PEGylation of liposomes resulted in the abovementioned decreased cell uptake, functionalization with <i>hb</i>PG lead to enhanced macrophage interaction-both in the media with and without proteins. In comparison to other nanocarrier systems, this seems to be a liposome-specific effect related to the low amount of adsorbed proteins.
- Addresses
- Max Planck Institute for Polymer Research , Ackermannweg 10 , 55128 Mainz , Germany.
- Autoren
- Claudia Weber
- Matthias Voigt
- Johanna Simon
- Ann-Kathrin Danner
- Holger Frey
- Volker Mailänder
- Mark Helm
- Svenja Morsbach
- Katharina Landfester
- DOI
- 10.1021/acs.biomac.9b00539
- eISSN
- 1526-4602
- Externe Identifier
- PubMed Identifier: 31268685
- PubMed Central ID: PMC6750830
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SFB1066
- Open access
- true
- ISSN
- 1525-7797
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Biomacromolecules
- Schlüsselwörter
- Macrophages
- Animals
- Humans
- Mice
- Polyethylene Glycols
- Polymers
- Liposomes
- Drug Carriers
- Biological Transport
- Nanoparticles
- Hydrophobic and Hydrophilic Interactions
- Protein Corona
- RAW 264.7 Cells
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2019
- Open access status
- Open Access
- Paginierung
- 2989 - 2999
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum der Datenerfassung
- 2019
- Titel
- Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 20
Files
https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.9b00539 https://europepmc.org/articles/PMC6750830?pdf=render
Data source: Europe PubMed Central
- Abstract
- Liposomes are established drug carriers that are employed to transport and deliver hydrophilic drugs in the body. To minimize unspecific cellular uptake, nanocarriers are commonly modified with poly(ethylene glycol) (PEG), which is known to minimize unspecific protein adsorption. However, to date, it has not been studied whether this is an intrinsic and specific property of PEG or if it can be transferred to hyperbranched polyglycerol (hbPG) as well. Additionally, it remains unclear if the reduction of unspecific cell uptake is independent of the "basic" carrier at which a surface functionalization with polymers is usually applied. Therefore, we studied the protein corona of differently functionalized liposomes (unfunctionalized vs PEG or hbPG-functionalized) using PEGylated and PGylated lipids. Their cellular uptake in macrophages was compared. For all three liposomal samples, rather similar protein corona compositions were found, and also-more importantly-the total amount of proteins adsorbed was very low compared to other nanoparticles. Interestingly, the cellular uptake was then significantly changed by the surface functionalization itself, despite the adsorption of a small amount of proteins: although the PEGylation of liposomes resulted in the abovementioned decreased cell uptake, functionalization with hbPG lead to enhanced macrophage interaction-both in the media with and without proteins. In comparison to other nanocarrier systems, this seems to be a liposome-specific effect related to the low amount of adsorbed proteins.
- Autoren
- Claudia Weber
- Matthias Voigt
- Johanna Simon
- Ann-Kathrin Danner
- Holger Frey
- Volker Mailänder
- Mark Helm
- Svenja Morsbach
- Katharina Landfester
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31268685
- DOI
- 10.1021/acs.biomac.9b00539
- eISSN
- 1526-4602
- Externe Identifier
- PubMed Central ID: PMC6750830
- Ausgabe der Veröffentlichung
- 8
- Zeitschrift
- Biomacromolecules
- Schlüsselwörter
- Animals
- Biological Transport
- Drug Carriers
- Humans
- Hydrophobic and Hydrophilic Interactions
- Liposomes
- Macrophages
- Mice
- Nanoparticles
- Polyethylene Glycols
- Polymers
- Protein Corona
- RAW 264.7 Cells
- Sprache
- eng
- Country
- United States
- Paginierung
- 2989 - 2999
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 20
Data source: PubMed
- Beziehungen:
- Property of