Stability of Alkyl Chain-Mediated Lipid Anchoring in Liposomal Membranes
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Lukas Gleue
- Jonathan Schupp
- Niklas Zimmer
- Eyleen Becker
- Holger Frey
- Andrea Tuettenberg
- Mark Helm
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000584214100001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.3390/cells9102213
- eISSN
- 2073-4409
- Externe Identifier
- Clarivate Analytics Document Solution ID: OJ8OU
- PubMed Identifier: 33003620
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- CELLS
- Schlüsselwörter
- liposomes
- click chemistry
- polyglycerol
- bioconjugates
- drug delivery
- Artikelnummer
- ARTN 2213
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Titel
- Stability of Alkyl Chain-Mediated Lipid Anchoring in Liposomal Membranes
- Sub types
- Article
- Ausgabe der Zeitschrift
- 9
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:p>Lipid exchange among biological membranes, lipoprotein particles, micelles, and liposomes is an important yet underrated phenomenon with repercussions throughout the life sciences. The premature loss of lipid molecules from liposomal formulations severely impacts therapeutic applications of the latter and thus limits the type of lipids and lipid conjugates available for fine-tuning liposomal properties. While cholesterol derivatives, with their irregular lipophilic surface shape, are known to readily undergo lipid exchange and interconvert, e.g., with serum, the situation is unclear for lipids with regular, linear-shaped alkyl chains. This study compares the propensity of fluorescence-labeled lipid conjugates of systematically varied lengths to migrate from liposomal particles consisting mainly of egg phosphatidyl choline 3 (EPC3) and cholesterol into biomembranes. We show that dialkyl glyceryl lipids with chains of 18–20 methylene units are inherently stable in liposomal membranes. In contrast, C16 lipids show some lipid exchange, albeit significantly less than comparable cholesterol conjugates. Remarkably, the C18 chain length, which confers noticeable anchor stability, corresponds to the typical chain length in biological membranes.</jats:p>
- Autoren
- Lukas Gleue
- Jonathan Schupp
- Niklas Zimmer
- Eyleen Becker
- Holger Frey
- Andrea Tuettenberg
- Mark Helm
- DOI
- 10.3390/cells9102213
- eISSN
- 2073-4409
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cells
- Sprache
- en
- Online publication date
- 2020
- Paginierung
- 2213 - 2213
- Status
- Published online
- Herausgeber
- MDPI AG
- Herausgeber URL
- http://dx.doi.org/10.3390/cells9102213
- Datum der Datenerfassung
- 2020
- Titel
- Stability of Alkyl Chain-Mediated Lipid Anchoring in Liposomal Membranes
- Ausgabe der Zeitschrift
- 9
Data source: Crossref
- Abstract
- Lipid exchange among biological membranes, lipoprotein particles, micelles, and liposomes is an important yet underrated phenomenon with repercussions throughout the life sciences. The premature loss of lipid molecules from liposomal formulations severely impacts therapeutic applications of the latter and thus limits the type of lipids and lipid conjugates available for fine-tuning liposomal properties. While cholesterol derivatives, with their irregular lipophilic surface shape, are known to readily undergo lipid exchange and interconvert, e.g., with serum, the situation is unclear for lipids with regular, linear-shaped alkyl chains. This study compares the propensity of fluorescence-labeled lipid conjugates of systematically varied lengths to migrate from liposomal particles consisting mainly of egg phosphatidyl choline 3 (EPC3) and cholesterol into biomembranes. We show that dialkyl glyceryl lipids with chains of 18-20 methylene units are inherently stable in liposomal membranes. In contrast, C16 lipids show some lipid exchange, albeit significantly less than comparable cholesterol conjugates. Remarkably, the C18 chain length, which confers noticeable anchor stability, corresponds to the typical chain length in biological membranes.
- Addresses
- Institute of Pharmaceutical and Biomedical Science, Johannes Gutenberg-University Mainz, 55128 Mainz, Germany.
- Autoren
- Lukas Gleue
- Jonathan Schupp
- Niklas Zimmer
- Eyleen Becker
- Holger Frey
- Andrea Tuettenberg
- Mark Helm
- DOI
- 10.3390/cells9102213
- eISSN
- 2073-4409
- Externe Identifier
- PubMed Identifier: 33003620
- PubMed Central ID: PMC7599733
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: SFB1066 Project A7
- Open access
- true
- ISSN
- 2073-4409
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cells
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Glycerol
- Polymers
- Lipids
- Membranes, Artificial
- Liposomes
- Microscopy, Fluorescence
- Drug Delivery Systems
- Flow Cytometry
- Click Chemistry
- Dynamic Light Scattering
- Sprache
- eng
- Medium
- Electronic
- Online publication date
- 2020
- Open access status
- Open Access
- Paginierung
- E2213
- Datum der Veröffentlichung
- 2020
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2020
- Titel
- Stability of Alkyl Chain-Mediated Lipid Anchoring in Liposomal Membranes.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 9
Files
https://www.mdpi.com/2073-4409/9/10/2213/pdf?version=1601446920 https://europepmc.org/articles/PMC7599733?pdf=render
Data source: Europe PubMed Central
- Abstract
- Lipid exchange among biological membranes, lipoprotein particles, micelles, and liposomes is an important yet underrated phenomenon with repercussions throughout the life sciences. The premature loss of lipid molecules from liposomal formulations severely impacts therapeutic applications of the latter and thus limits the type of lipids and lipid conjugates available for fine-tuning liposomal properties. While cholesterol derivatives, with their irregular lipophilic surface shape, are known to readily undergo lipid exchange and interconvert, e.g., with serum, the situation is unclear for lipids with regular, linear-shaped alkyl chains. This study compares the propensity of fluorescence-labeled lipid conjugates of systematically varied lengths to migrate from liposomal particles consisting mainly of egg phosphatidyl choline 3 (EPC3) and cholesterol into biomembranes. We show that dialkyl glyceryl lipids with chains of 18-20 methylene units are inherently stable in liposomal membranes. In contrast, C16 lipids show some lipid exchange, albeit significantly less than comparable cholesterol conjugates. Remarkably, the C18 chain length, which confers noticeable anchor stability, corresponds to the typical chain length in biological membranes.
- Date of acceptance
- 2020
- Autoren
- Lukas Gleue
- Jonathan Schupp
- Niklas Zimmer
- Eyleen Becker
- Holger Frey
- Andrea Tuettenberg
- Mark Helm
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/33003620
- DOI
- 10.3390/cells9102213
- eISSN
- 2073-4409
- Externe Identifier
- PubMed Central ID: PMC7599733
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Cells
- Schlüsselwörter
- bioconjugates
- click chemistry
- drug delivery
- liposomes
- polyglycerol
- Cell Line, Tumor
- Click Chemistry
- Drug Delivery Systems
- Dynamic Light Scattering
- Flow Cytometry
- Glycerol
- Humans
- Lipids
- Liposomes
- Membranes, Artificial
- Microscopy, Fluorescence
- Polymers
- Sprache
- eng
- Country
- Switzerland
- PII
- cells9102213
- Datum der Veröffentlichung
- 2020
- Status
- Published online
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2021
- Titel
- Stability of Alkyl Chain-Mediated Lipid Anchoring in Liposomal Membranes.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 9
Data source: PubMed
- Beziehungen:
- Property of