Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells

Publication type:
Journal article
Metadata:
Autoren
  • A Pulvermüller
  • A Giessl
  • M Heck
  • R Wottrich
  • A Schmitt
  • OP Ernst
  • HW Choe
  • KP Hofmann
  • U Wolfrum
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000174407500022&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1128/MCB.22.7.2194-2203.2002
eISSN
1098-5549
Externe Identifier
  • Clarivate Analytics Document Solution ID: 531GK
  • PubMed Identifier: 11884606
ISSN
0270-7306
Ausgabe der Veröffentlichung
7
Zeitschrift
MOLECULAR AND CELLULAR BIOLOGY
Paginierung
2194 - 2203
Datum der Veröffentlichung
2002
Status
Published
Titel
Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells
Sub types
  • Article
Ausgabe der Zeitschrift
22

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Alexander Pulvermüller
  • Andreas Gießl
  • Martin Heck
  • Ralf Wottrich
  • Angelika Schmitt
  • Oliver Peter Ernst
  • Hui-Woog Choe
  • Klaus Peter Hofmann
  • Uwe Wolfrum
DOI
10.1128/mcb.22.7.2194-2203.2002
eISSN
1098-5549
Ausgabe der Veröffentlichung
7
Zeitschrift
Molecular and Cellular Biology
Sprache
en
Online publication date
2023
Paginierung
2194 - 2203
Datum der Veröffentlichung
2002
Status
Published
Herausgeber
Informa UK Limited
Herausgeber URL
http://dx.doi.org/10.1128/mcb.22.7.2194-2203.2002
Datum der Datenerfassung
2023
Titel
Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells
Ausgabe der Zeitschrift
22

Data source: Crossref

Abstract
Photoexcitation of rhodopsin activates a heterotrimeric G-protein cascade leading to cyclic GMP hydrolysis in vertebrate photoreceptors. Light-induced exchanges of the visual G-protein transducin between the outer and inner segment of rod photoreceptors occur through the narrow connecting cilium. Here we demonstrate that transducin colocalizes with the Ca(2+)-binding protein centrin 1 in a specific domain of this cilium. Coimmunoprecipitation, centrifugation, centrin overlay, size exclusion chromatography, and kinetic light-scattering experiments indicate that Ca(2+)-activated centrin 1 binds with high affinity and specificity to transducin. The assembly of centrin-G-protein complex is mediated by the betagamma-complex. The Ca(2+)-dependent assembly of a G protein with centrin is a novel aspect of the supply of signaling proteins in sensory cells and a potential link between molecular translocations and signal transduction in general.
Addresses
  • Institut für Medizinische Physik und Biophysik, Humboldt-Universität zu Berlin, Germany. alexander.pulvermueller@charite.de
Autoren
  • Alexander Pulvermüller
  • Andreas Giessl
  • Martin Heck
  • Ralf Wottrich
  • Angelika Schmitt
  • Oliver Peter Ernst
  • Hui-Woog Choe
  • Klaus Peter Hofmann
  • Uwe Wolfrum
DOI
10.1128/mcb.22.7.2194-2203.2002
eISSN
1098-5549
Externe Identifier
  • PubMed Identifier: 11884606
  • PubMed Central ID: PMC133667
Open access
false
ISSN
0270-7306
Ausgabe der Veröffentlichung
7
Zeitschrift
Molecular and cellular biology
Schlüsselwörter
  • Retina
  • Animals
  • Mice, Inbred C57BL
  • Cattle
  • Mice
  • Rats
  • Rats, Sprague-Dawley
  • Calcium
  • Macromolecular Substances
  • Heterotrimeric GTP-Binding Proteins
  • Transducin
  • Calcium-Binding Proteins
  • Chromosomal Proteins, Non-Histone
  • Microscopy, Immunoelectron
  • Precipitin Tests
  • Centrifugation
  • Chromatography, Gel
  • Signal Transduction
  • Protein Binding
  • Substrate Specificity
  • Molecular Weight
  • Light
  • Scattering, Radiation
  • Photoreceptor Cells, Vertebrate
Sprache
eng
Medium
Print
Paginierung
2194 - 2203
Datum der Veröffentlichung
2002
Status
Published
Datum der Datenerfassung
2002
Titel
Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
22

Data source: Europe PubMed Central

Abstract
Photoexcitation of rhodopsin activates a heterotrimeric G-protein cascade leading to cyclic GMP hydrolysis in vertebrate photoreceptors. Light-induced exchanges of the visual G-protein transducin between the outer and inner segment of rod photoreceptors occur through the narrow connecting cilium. Here we demonstrate that transducin colocalizes with the Ca(2+)-binding protein centrin 1 in a specific domain of this cilium. Coimmunoprecipitation, centrifugation, centrin overlay, size exclusion chromatography, and kinetic light-scattering experiments indicate that Ca(2+)-activated centrin 1 binds with high affinity and specificity to transducin. The assembly of centrin-G-protein complex is mediated by the betagamma-complex. The Ca(2+)-dependent assembly of a G protein with centrin is a novel aspect of the supply of signaling proteins in sensory cells and a potential link between molecular translocations and signal transduction in general.
Autoren
  • Alexander Pulvermüller
  • Andreas Giessl
  • Martin Heck
  • Ralf Wottrich
  • Angelika Schmitt
  • Oliver Peter Ernst
  • Hui-Woog Choe
  • Klaus Peter Hofmann
  • Uwe Wolfrum
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/11884606
DOI
10.1128/MCB.22.7.2194-2203.2002
Externe Identifier
  • PubMed Central ID: PMC133667
ISSN
0270-7306
Ausgabe der Veröffentlichung
7
Zeitschrift
Mol Cell Biol
Schlüsselwörter
  • Animals
  • Calcium
  • Calcium-Binding Proteins
  • Cattle
  • Centrifugation
  • Chromatography, Gel
  • Chromosomal Proteins, Non-Histone
  • Heterotrimeric GTP-Binding Proteins
  • Light
  • Macromolecular Substances
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Immunoelectron
  • Molecular Weight
  • Photoreceptor Cells, Vertebrate
  • Precipitin Tests
  • Protein Binding
  • Rats
  • Rats, Sprague-Dawley
  • Retina
  • Scattering, Radiation
  • Signal Transduction
  • Substrate Specificity
  • Transducin
Sprache
eng
Country
United States
Paginierung
2194 - 2203
Datum der Veröffentlichung
2002
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2002
Titel
Calcium-dependent assembly of centrin-G-protein complex in photoreceptor cells.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
22

Data source: PubMed

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