Divergent distribution in vascular and avascular mammalian retinae links neuroglobin to cellular respiration

Autoren

Anke Bentmann
Marc Schmidt
Stefan Reuss
Uwe Wolfrum
Thomas Hankeln
Thorsten Burmester

DOI

10.1074/jbc.m501338200

ISSN

0021-9258

Ausgabe der Veröffentlichung

21

Zeitschrift

Journal of Biological Chemistry

Sprache

en

Paginierung

20660 - 20665

Datum der Veröffentlichung

2005

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1074/jbc.m501338200

Datum der Datenerfassung

2022

Titel

Divergent Distribution in Vascular and Avascular Mammalian Retinae Links Neuroglobin to Cellular Respiration

Ausgabe der Zeitschrift

280

Data source: Crossref

Abstract

The visual function of the vertebrate retina relies on sufficient supply with oxygen. Neuroglobin is a respiratory protein thought to play an essential role in oxygen homeostasis of neuronal cells. For further understanding of its function, we compared the distribution of neuroglobin and mitochondria in both vascular and avascular mammalian retinae. In the vascular retinae of mouse and rat, oxygen is supplied by the outer choroidal, deep retinal, and inner capillaries. We show that in this type of retina, mitochondria are concentrated in the inner segments of photoreceptor cells, the outer and the inner plexiform layers, and the ganglion cell layer. These are the same regions in which oxygen consumption takes place and in which neuroglobin is present at high levels. In the avascular retina of guinea pig the deep retinal and inner capillaries are absent. Therefore, only the inner segments of the photoreceptors adjacent to choroidal capillaries display an oxidative metabolism. We demonstrate that in the retina of guinea pigs both neuroglobin and mitochondria are restricted to this layer. Our results clearly demonstrate an association of neuroglobin and mitochondria, thus supporting the hypothesis that neuroglobin is a respiratory protein that supplies oxygen to the respiratory chain.

Addresses

Department of Molecular Animal Physiology, Institute of Zoology, Johannes Gutenberg-University of Mainz, D-55099 Mainz, Germany.

Autoren

Anke Bentmann
Marc Schmidt
Stefan Reuss
Uwe Wolfrum
Thomas Hankeln
Thorsten Burmester

DOI

10.1074/jbc.m501338200

eISSN

1083-351X

Externe Identifier

PubMed Identifier: 15793311

Open access

false

ISSN

0021-9258

Ausgabe der Veröffentlichung

21

Zeitschrift

The Journal of biological chemistry

Schlüsselwörter

Capillaries
Retinal Vessels
Retina
Choroid
Mitochondria
Animals
Mice, Inbred BALB C
Guinea Pigs
Mice
Rats
Cytochromes c
Globins
Nerve Tissue Proteins
DNA, Complementary
Antibodies, Monoclonal
Immunohistochemistry
Cloning, Molecular
Sequence Alignment
Amino Acid Sequence
Oxygen Consumption
Tissue Distribution
Molecular Sequence Data
Photoreceptor Cells
Neuroglobin

Sprache

eng

Medium

Print-Electronic

Online publication date

2005

Paginierung

20660 - 20665

Datum der Veröffentlichung

2005

Status

Published

Publisher licence

CC BY

Datum der Datenerfassung

2005

Titel

Divergent distribution in vascular and avascular mammalian retinae links neuroglobin to cellular respiration.

Sub types

Comparative Study
Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

280

Data source: Europe PubMed Central

Abstract

The visual function of the vertebrate retina relies on sufficient supply with oxygen. Neuroglobin is a respiratory protein thought to play an essential role in oxygen homeostasis of neuronal cells. For further understanding of its function, we compared the distribution of neuroglobin and mitochondria in both vascular and avascular mammalian retinae. In the vascular retinae of mouse and rat, oxygen is supplied by the outer choroidal, deep retinal, and inner capillaries. We show that in this type of retina, mitochondria are concentrated in the inner segments of photoreceptor cells, the outer and the inner plexiform layers, and the ganglion cell layer. These are the same regions in which oxygen consumption takes place and in which neuroglobin is present at high levels. In the avascular retina of guinea pig the deep retinal and inner capillaries are absent. Therefore, only the inner segments of the photoreceptors adjacent to choroidal capillaries display an oxidative metabolism. We demonstrate that in the retina of guinea pigs both neuroglobin and mitochondria are restricted to this layer. Our results clearly demonstrate an association of neuroglobin and mitochondria, thus supporting the hypothesis that neuroglobin is a respiratory protein that supplies oxygen to the respiratory chain.

Autoren

Anke Bentmann
Marc Schmidt
Stefan Reuss
Uwe Wolfrum
Thomas Hankeln
Thorsten Burmester

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/15793311

DOI

10.1074/jbc.M501338200

ISSN

0021-9258

Ausgabe der Veröffentlichung

21

Zeitschrift

J Biol Chem

Schlüsselwörter

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Capillaries
Choroid
Cloning, Molecular
Cytochromes c
DNA, Complementary
Globins
Guinea Pigs
Immunohistochemistry
Mice
Mice, Inbred BALB C
Mitochondria
Molecular Sequence Data
Nerve Tissue Proteins
Neuroglobin
Oxygen Consumption
Photoreceptor Cells
Rats
Retina
Retinal Vessels
Sequence Alignment
Tissue Distribution

Sprache

eng

Country

United States

Paginierung

20660 - 20665

PII

S0021-9258(20)64070-4

Datum der Veröffentlichung

2005

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2005

Titel

Divergent distribution in vascular and avascular mammalian retinae links neuroglobin to cellular respiration.

Sub types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

280

Data source: PubMed

Divergent distribution in vascular and avascular mammalian retinae links neuroglobin to cellular respiration

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