MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes

Abstract

Defects of the myosin VIIa motor protein cause deafness and retinal anomalies in humans and mice. We report on the identification of a novel myosin‐VIIa‐interacting protein that we have named MyRIP (myosin‐VIIa‐ and Rab‐interacting protein), since it also binds to Rab27A in a GTP‐dependent manner. In the retinal pigment epithelium cells, MyRIP, myosin VIIa and Rab27A are associated with melanosomes. In transfected PC12 cells, overexpression of MyRIP was shown to interfere with the myosin VIIa tail localization. We propose that a molecular complex composed of Rab27A, MyRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosinVIIa‐defective mice.

Autoren

Aziz El‐Amraoui
Jean‐Sébastien Schonn
Polonca Küssel‐Andermann
Stéphane Blanchard
Claire Desnos
Jean‐Pierre Henry
Uwe Wolfrum
François Darchen
Christine Petit

DOI

10.1093/embo-reports/kvf090

eISSN

1469-3178

ISSN

1469-221X

Ausgabe der Veröffentlichung

5

Zeitschrift

EMBO reports

Sprache

en

Online publication date

2002

Paginierung

463 - 470

Datum der Veröffentlichung

2002

Status

Published

Herausgeber

Springer Science and Business Media LLC

Herausgeber URL

http://dx.doi.org/10.1093/embo-reports/kvf090

Datum der Datenerfassung

2023

Titel

MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes

Ausgabe der Zeitschrift

3

Data source: Crossref

Abstract

Defects of the myosin VIIa motor protein cause deafness and retinal anomalies in humans and mice. We report on the identification of a novel myosin-VIIa-interacting protein that we have named MyRIP (myosin-VIIa- and Rab-interacting protein), since it also binds to Rab27A in a GTP-dependent manner. In the retinal pigment epithelium cells, MyRIP, myosin VIIa and Rab27A are associated with melanosomes. In transfected PC12 cells, overexpression of MyRIP was shown to interfere with the myosin VIIa tail localization. We propose that a molecular complex composed of Rab27A, MyRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosinVIIa-defective mice.

Addresses

Unité de Génétique des Déficits Sensoriels, CNRS URA 1968, Institut Pasteur, 25 rue du Dr Roux, F-5724 Paris cedex 15, France.

Autoren

Aziz El-Amraoui
Jéan-Sebastien Schonn
Polonca Küssel-Andermann
Stéphane Blanchard
Claire Desnos
Jean-Pierre Henry
Uwe Wolfrum
François Darchen
Christine Petit

DOI

10.1093/embo-reports/kvf090

eISSN

1469-3178

Externe Identifier

PubMed Identifier: 11964381
PubMed Central ID: PMC1084103

Open access

false

ISSN

1469-221X

Ausgabe der Veröffentlichung

5

Zeitschrift

EMBO reports

Schlüsselwörter

Retina
Melanosomes
Animals
Humans
Mice
Myosins
rab GTP-Binding Proteins
Two-Hybrid System Techniques
Sequence Alignment
Organ Specificity
Amino Acid Sequence
Gene Library
Molecular Sequence Data
Dyneins
rab27 GTP-Binding Proteins
Myosin VIIa

Sprache

eng

Medium

Print-Electronic

Online publication date

2002

Paginierung

463 - 470

Datum der Veröffentlichung

2002

Status

Published

Datum der Datenerfassung

2002

Titel

MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes.

Sub types

other
Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

3

Files

http://embor.embopress.org/content/embor/3/5/463.full.pdf https://europepmc.org/articles/PMC1084103?pdf=render

Data source: Europe PubMed Central

Abstract

Defects of the myosin VIIa motor protein cause deafness and retinal anomalies in humans and mice. We report on the identification of a novel myosin-VIIa-interacting protein that we have named MyRIP (myosin-VIIa- and Rab-interacting protein), since it also binds to Rab27A in a GTP-dependent manner. In the retinal pigment epithelium cells, MyRIP, myosin VIIa and Rab27A are associated with melanosomes. In transfected PC12 cells, overexpression of MyRIP was shown to interfere with the myosin VIIa tail localization. We propose that a molecular complex composed of Rab27A, MyRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosinVIIa-defective mice.

Autoren

Aziz El-Amraoui
Jéan-Sebastien Schonn
Polonca Küssel-Andermann
Stéphane Blanchard
Claire Desnos
Jean-Pierre Henry
Uwe Wolfrum
François Darchen
Christine Petit

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/11964381

DOI

10.1093/embo-reports/kvf090

Externe Identifier

PubMed Central ID: PMC1084103

ISSN

1469-221X

Ausgabe der Veröffentlichung

5

Zeitschrift

EMBO Rep

Schlüsselwörter

Amino Acid Sequence
Animals
Dyneins
Gene Library
Humans
Melanosomes
Mice
Molecular Sequence Data
Myosin VIIa
Myosins
Organ Specificity
Retina
Sequence Alignment
Two-Hybrid System Techniques
rab GTP-Binding Proteins
rab27 GTP-Binding Proteins

Sprache

eng

Country

England

Paginierung

463 - 470

PII

kvf090

Datum der Veröffentlichung

2002

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2002

Titel

MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

3

Data source: PubMed

MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes

Files

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