CENTRIN IN THE PHOTORECEPTOR CELLS OF MAMMALIAN RETINAE
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- U WOLFRUM
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:A1995RX03200006&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/cm.970320107
- Externe Identifier
- Clarivate Analytics Document Solution ID: RX032
- PubMed Identifier: 8674134
- ISSN
- 0886-1544
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- CELL MOTILITY AND THE CYTOSKELETON
- Schlüsselwörter
- RETINA
- PHOTORECEPTOR CELLS
- CYTOSKELETON
- CENTRIN
- CA2+-BINDING PROTEINS
- MAMMALS
- Paginierung
- 55 - 64
- Datum der Veröffentlichung
- 1995
- Status
- Published
- Titel
- CENTRIN IN THE PHOTORECEPTOR CELLS OF MAMMALIAN RETINAE
- Sub types
- Article
- Ausgabe der Zeitschrift
- 32
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Photoreceptor cells of vertebrate retinae are highly specialized ciliary cells. Their non‐motile ciliated structure is restricted to the so‐called connecting cilium at the joint between the light sensitive outer segment and the metabolically active inner segment. Extensive bidirectional intracellular transport between both segments is forced to occur through this tight connecting cilium. In the present study it is shown that the Ca<jats:sup>2+</jats:sup>‐binding, phospho‐protein centrin is present in mammalian retinae. Western blot and immunoprecipitation experiments reveal that anti‐centrin antibodies react with purified photoreceptor cell fractions of retinae in bands at a molecular weight of 20 kDa, the molecular weight of centrins found in other cells. Indirect immunofluorescence analysis of cryosections through retinae of different mammalian species show that centrin is present only in centrosomes and basal bodies but also more extensively at the linkage between the inner and the outer segment of the photoreceptor cells. Immunocytological studies on isolated rod cells and immunoelectron microscopy clearly demonstrate a unique presence of centrin in the connecting cilium of photoreceptor cells. High molecular identity between centrins in lower eukaryotes and mammals indicates that centrin may play a role in cellular motility and/or in microtubule severing in the mammalian retina. © 1995 Wiley‐Liss, Inc.</jats:p>
- Autoren
- Uwe Wolfrum
- DOI
- 10.1002/cm.970320107
- ISSN
- 0886-1544
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Cell Motility
- Sprache
- en
- Online publication date
- 2005
- Paginierung
- 55 - 64
- Datum der Veröffentlichung
- 1995
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/cm.970320107
- Datum der Datenerfassung
- 2023
- Titel
- Centrin in the photoreceptor cells of mammalian retinae
- Ausgabe der Zeitschrift
- 32
Data source: Crossref
- Abstract
- Photoreceptor cells of vertebrate retinae are highly specialized ciliary cells. Their non-motile ciliated structure is restricted to the so-called connecting cilium at the joint between the light sensitive outer segment and the metabolically active inner segment. Extensive bidirectional intracellular transport between both segments is forced to occur through this tight connecting cilium. In the present study it is shown that the CA2+-binding, phospho-protein centrin is present in mammalian retinae. Western blot and immunoprecipitation reveal that anti-centrin antibodies react with purified photoreceptor cell fractions of retinae in bands at a molecular weight of 20 kDa, the molecular weight of centrins found in other cells. Indirect immunofluorescence analysis of cryosections through retinae of different mammalian species show that centrin is present only in centrosomes and basal bodies but also more extensively at the linkage between the inner and the outer segment of the photoreceptor cells. Immunocytological studies on isolated rod cells and immunoelectron microscopy clearly demonstrate a unique presence of centrin in the connecting cilium of photoreceptor cells. High molecular identity between centrins in lower eukaryotes and mammals indicates that centrin may play a role in cellular motility and/or in microtubule severing in the mammalian retina.
- Addresses
- Zoologisches Institut I, Universität Karlsruhe (TH), Federal Republic of Germany.
- Autoren
- U Wolfrum
- DOI
- 10.1002/cm.970320107
- eISSN
- 1097-0169
- Externe Identifier
- PubMed Identifier: 8674134
- Open access
- false
- ISSN
- 0886-1544
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Cell motility and the cytoskeleton
- Schlüsselwörter
- Retina
- Cilia
- Cytoskeleton
- Centrosome
- Animals
- Cattle
- Calcium-Binding Proteins
- Contractile Proteins
- Chromosomal Proteins, Non-Histone
- Microscopy, Immunoelectron
- Fluorescent Antibody Technique
- Blotting, Western
- Precipitin Tests
- Photoreceptor Cells
- Sprache
- eng
- Medium
- Paginierung
- 55 - 64
- Datum der Veröffentlichung
- 1995
- Status
- Published
- Datum der Datenerfassung
- 1995
- Titel
- Centrin in the photoreceptor cells of mammalian retinae.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 32
Data source: Europe PubMed Central
- Abstract
- Photoreceptor cells of vertebrate retinae are highly specialized ciliary cells. Their non-motile ciliated structure is restricted to the so-called connecting cilium at the joint between the light sensitive outer segment and the metabolically active inner segment. Extensive bidirectional intracellular transport between both segments is forced to occur through this tight connecting cilium. In the present study it is shown that the CA2+-binding, phospho-protein centrin is present in mammalian retinae. Western blot and immunoprecipitation reveal that anti-centrin antibodies react with purified photoreceptor cell fractions of retinae in bands at a molecular weight of 20 kDa, the molecular weight of centrins found in other cells. Indirect immunofluorescence analysis of cryosections through retinae of different mammalian species show that centrin is present only in centrosomes and basal bodies but also more extensively at the linkage between the inner and the outer segment of the photoreceptor cells. Immunocytological studies on isolated rod cells and immunoelectron microscopy clearly demonstrate a unique presence of centrin in the connecting cilium of photoreceptor cells. High molecular identity between centrins in lower eukaryotes and mammals indicates that centrin may play a role in cellular motility and/or in microtubule severing in the mammalian retina.
- Autoren
- U Wolfrum
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/8674134
- DOI
- 10.1002/cm.970320107
- ISSN
- 0886-1544
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Cell Motil Cytoskeleton
- Schlüsselwörter
- Animals
- Blotting, Western
- Calcium-Binding Proteins
- Cattle
- Centrosome
- Chromosomal Proteins, Non-Histone
- Cilia
- Contractile Proteins
- Cytoskeleton
- Fluorescent Antibody Technique
- Microscopy, Immunoelectron
- Photoreceptor Cells
- Precipitin Tests
- Retina
- Sprache
- eng
- Country
- United States
- Paginierung
- 55 - 64
- Datum der Veröffentlichung
- 1995
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 1996
- Titel
- Centrin in the photoreceptor cells of mammalian retinae.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 32
Data source: PubMed
- Beziehungen:
- Property of