Crystallization and preliminary X-ray studies of mouse centrin1
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- JH Park
- N Krauss
- A Pulvermüller
- P Scheerer
- W Höhne
- A Giessl
- U Wolfrum
- KP Hofmann
- OP Ernst
- HW Choe
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000232319100016&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1107/S1744309105011462
- eISSN
- 2053-230X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 970OI
- PubMed Identifier: 16511082
- Zeitschrift
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
- Paginierung
- 510 - 513
- Datum der Veröffentlichung
- 2005
- Status
- Published
- Titel
- Crystallization and preliminary X-ray studies of mouse centrin1
- Sub types
- Article
- Ausgabe der Zeitschrift
- 61
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Autoren
- Jung Hee Park
- Norbert Krauss
- Alexander Pulvermüller
- Patrick Scheerer
- Wolfgang Höhne
- Andreas Giessl
- Uwe Wolfrum
- Klaus Peter Hofmann
- Oliver Peter Ernst
- Hui-Woog Choe
- DOI
- 10.1107/s1744309105011462
- eISSN
- 1744-3091
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Online publication date
- 2005
- Paginierung
- 510 - 513
- Datum der Veröffentlichung
- 2005
- Status
- Published
- Herausgeber
- International Union of Crystallography (IUCr)
- Herausgeber URL
- http://dx.doi.org/10.1107/s1744309105011462
- Datum der Datenerfassung
- 2022
- Titel
- Crystallization and preliminary X-ray studies of mouse centrin1
- Ausgabe der Zeitschrift
- 61
Data source: Crossref
- Abstract
- Centrins belong to a family of Ca2+-binding EF-hand proteins that play a fundamental role in centrosome duplication and the function of cilia. To shed light on the structure-function relationship of these proteins, mouse centrin1 has been crystallized. The mouse centrin1 has been expressed in Escherichia coli as a GST-centrin fusion protein containing a thrombin protease cleavage site between the fusion partners. Two constructs with different linking-sequence lengths were expressed and purified. Thrombin cleavage yielded functional centrin1 and N-terminally extended centrin1 containing 25 additional residues upstream of its N-terminus. Only N-terminally extended centrin1 (MW approximately 22 240 Da) could be crystallized at room temperature, using 20-25%(w/v) PEG 1500, 5-10%(v/v) ethylene glycol and 1-2%(v/v) dioxane. Crystals were suitable for X-ray analysis, diffracting to 2.9 A at 295 K using a rotating-anode X-ray source. They belong to space group C2, with unit-cell parameters a = 60.7, b = 59.6, c = 58.3 A, beta = 109.4 degrees. Assuming the asymmetric cell to be occupied by one centrin1 molecule of 22.2 kDa, the unit cell contains 45% solvent with a crystal volume per protein weight, VM, of 2.2 A3 Da(-1).
- Addresses
- Institut für Medizinische Physik und Biophysik, Charité-Universitätsmedizin Berlin, Ziegelstrasse 5-9, D-10098 Berlin, Germany.
- Autoren
- Jung Hee Park
- Norbert Krauss
- Alexander Pulvermüller
- Patrick Scheerer
- Wolfgang Höhne
- Andreas Giessl
- Uwe Wolfrum
- Klaus Peter Hofmann
- Oliver Peter Ernst
- Hui-Woog Choe
- DOI
- 10.1107/s1744309105011462
- eISSN
- 1744-3091
- Externe Identifier
- PubMed Identifier: 16511082
- PubMed Central ID: PMC1952316
- Open access
- false
- Ausgabe der Veröffentlichung
- Pt 5
- Zeitschrift
- Acta crystallographica. Section F, Structural biology and crystallization communications
- Schlüsselwörter
- Animals
- Mice
- Escherichia coli
- Calcium-Binding Proteins
- Chromosomal Proteins, Non-Histone
- Recombinant Proteins
- Crystallization
- Crystallography, X-Ray
- Amino Acid Sequence
- Structure-Activity Relationship
- Dose-Response Relationship, Drug
- Kinetics
- Molecular Sequence Data
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2005
- Paginierung
- 510 - 513
- Datum der Veröffentlichung
- 2005
- Status
- Published
- Datum der Datenerfassung
- 2006
- Titel
- Crystallization and preliminary X-ray studies of mouse centrin1.
- Sub types
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 61
Data source: Europe PubMed Central
- Abstract
- Centrins belong to a family of Ca2+-binding EF-hand proteins that play a fundamental role in centrosome duplication and the function of cilia. To shed light on the structure-function relationship of these proteins, mouse centrin1 has been crystallized. The mouse centrin1 has been expressed in Escherichia coli as a GST-centrin fusion protein containing a thrombin protease cleavage site between the fusion partners. Two constructs with different linking-sequence lengths were expressed and purified. Thrombin cleavage yielded functional centrin1 and N-terminally extended centrin1 containing 25 additional residues upstream of its N-terminus. Only N-terminally extended centrin1 (MW approximately 22 240 Da) could be crystallized at room temperature, using 20-25%(w/v) PEG 1500, 5-10%(v/v) ethylene glycol and 1-2%(v/v) dioxane. Crystals were suitable for X-ray analysis, diffracting to 2.9 A at 295 K using a rotating-anode X-ray source. They belong to space group C2, with unit-cell parameters a = 60.7, b = 59.6, c = 58.3 A, beta = 109.4 degrees. Assuming the asymmetric cell to be occupied by one centrin1 molecule of 22.2 kDa, the unit cell contains 45% solvent with a crystal volume per protein weight, VM, of 2.2 A3 Da(-1).
- Date of acceptance
- 2005
- Autoren
- Jung Hee Park
- Norbert Krauss
- Alexander Pulvermüller
- Patrick Scheerer
- Wolfgang Höhne
- Andreas Giessl
- Uwe Wolfrum
- Klaus Peter Hofmann
- Oliver Peter Ernst
- Hui-Woog Choe
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/16511082
- DOI
- 10.1107/S1744309105011462
- eISSN
- 1744-3091
- Externe Identifier
- PubMed Central ID: PMC1952316
- Ausgabe der Veröffentlichung
- Pt 5
- Zeitschrift
- Acta Crystallogr Sect F Struct Biol Cryst Commun
- Schlüsselwörter
- Amino Acid Sequence
- Animals
- Calcium-Binding Proteins
- Chromosomal Proteins, Non-Histone
- Crystallization
- Crystallography, X-Ray
- Dose-Response Relationship, Drug
- Escherichia coli
- Kinetics
- Mice
- Molecular Sequence Data
- Recombinant Proteins
- Structure-Activity Relationship
- Sprache
- eng
- Country
- England
- Paginierung
- 510 - 513
- PII
- S1744309105011462
- Datum der Veröffentlichung
- 2005
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2006
- Titel
- Crystallization and preliminary X-ray studies of mouse centrin1.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 61
Data source: PubMed
- Beziehungen:
- Property of