Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells

Autoren

Vincent Michel
Richard J Goodyear
Dominique Weil
Walter Marcotti
Isabelle Perfettini
Uwe Wolfrum
Corné J Kros
Guy P Richardson
Christine Petit

DOI

10.1016/j.ydbio.2005.01.014

ISSN

0012-1606

Ausgabe der Veröffentlichung

2

Zeitschrift

Developmental Biology

Sprache

en

Paginierung

281 - 294

Datum der Veröffentlichung

2005

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1016/j.ydbio.2005.01.014

Datum der Datenerfassung

2019

Titel

Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells

Ausgabe der Zeitschrift

280

Data source: Crossref

Abstract

Cadherin 23 is required for normal development of the sensory hair bundle, and recent evidence suggests it is a component of the tip links, filamentous structures thought to gate the hair cells' mechano-electrical transducer channels. Antibodies against unique peptide epitopes were used to study the properties of cadherin 23 and its spatio-temporal expression patterns in developing cochlear hair cells. In the rat, intra- and extracellular domain epitopes are readily detected in the developing hair bundle between E18 and P5, and become progressively restricted to the distal tip of the hair bundle. From P13 onwards, these epitopes are no longer detected in hair bundles, but immunoreactivity is observed in the apical, vesicle-rich, pericuticular region of the hair cell. In the P2-P3 mouse cochlea, immunogold labeling reveals cadherin 23 is associated with kinocilial links and transient lateral links located between and within stereociliary rows. At this stage, the cadherin 23 ectodomain epitope remains on the hair bundle following BAPTA or La(3+) treatment, but is lost following exposure to the protease subtilisin. In contrast, mechano-electrical transduction is abolished by BAPTA but unaffected by subtilisin. These results suggest cadherin 23 is associated with transient lateral links that have properties distinct from those of the tip-link.

Addresses

Unité de Génétique des Déficits Sensoriels, INSERM U587, Institut Pasteur, Paris, France.

Autoren

Vincent Michel
Richard J Goodyear
Dominique Weil
Walter Marcotti
Isabelle Perfettini
Uwe Wolfrum
Corné J Kros
Guy P Richardson
Christine Petit

DOI

10.1016/j.ydbio.2005.01.014

eISSN

1095-564X

Externe Identifier

PubMed Identifier: 15882573

Open access

false

ISSN

0012-1606

Ausgabe der Veröffentlichung

2

Zeitschrift

Developmental biology

Schlüsselwörter

Cochlea
Cells, Cultured
Cytoplasm
Animals
Mice
Rats
Lanthanum
Egtazic Acid
Subtilisin
Cadherins
Epitopes
Indicators and Reagents
Microscopy, Electron
Microscopy, Immunoelectron
Microscopy, Fluorescence
Immunohistochemistry
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Gene Expression Regulation, Developmental
Protein Structure, Tertiary
Models, Genetic
Time Factors
Ear, Inner
Hair Cells, Auditory

Sprache

eng

Medium

Print

Paginierung

281 - 294

Datum der Veröffentlichung

2005

Status

Published

Datum der Datenerfassung

2005

Titel

Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

280

Data source: Europe PubMed Central

Abstract

Cadherin 23 is required for normal development of the sensory hair bundle, and recent evidence suggests it is a component of the tip links, filamentous structures thought to gate the hair cells' mechano-electrical transducer channels. Antibodies against unique peptide epitopes were used to study the properties of cadherin 23 and its spatio-temporal expression patterns in developing cochlear hair cells. In the rat, intra- and extracellular domain epitopes are readily detected in the developing hair bundle between E18 and P5, and become progressively restricted to the distal tip of the hair bundle. From P13 onwards, these epitopes are no longer detected in hair bundles, but immunoreactivity is observed in the apical, vesicle-rich, pericuticular region of the hair cell. In the P2-P3 mouse cochlea, immunogold labeling reveals cadherin 23 is associated with kinocilial links and transient lateral links located between and within stereociliary rows. At this stage, the cadherin 23 ectodomain epitope remains on the hair bundle following BAPTA or La(3+) treatment, but is lost following exposure to the protease subtilisin. In contrast, mechano-electrical transduction is abolished by BAPTA but unaffected by subtilisin. These results suggest cadherin 23 is associated with transient lateral links that have properties distinct from those of the tip-link.

Date of acceptance

2005

Autoren

Vincent Michel
Richard J Goodyear
Dominique Weil
Walter Marcotti
Isabelle Perfettini
Uwe Wolfrum
Corné J Kros
Guy P Richardson
Christine Petit

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/15882573

DOI

10.1016/j.ydbio.2005.01.014

ISSN

0012-1606

Ausgabe der Veröffentlichung

2

Zeitschrift

Dev Biol

Schlüsselwörter

Animals
Cadherins
Cells, Cultured
Cochlea
Cytoplasm
Ear, Inner
Egtazic Acid
Epitopes
Gene Expression Regulation, Developmental
Hair Cells, Auditory
Immunohistochemistry
Indicators and Reagents
Lanthanum
Mice
Microscopy, Electron
Microscopy, Fluorescence
Microscopy, Immunoelectron
Models, Genetic
Protein Structure, Tertiary
Rats
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Subtilisin
Time Factors

Sprache

eng

Country

United States

Paginierung

281 - 294

PII

S0012-1606(05)00041-2

Datum der Veröffentlichung

2005

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2005

Titel

Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

280

Data source: PubMed

Cadherin 23 is a component of the transient lateral links in the developing hair bundles of cochlear sensory cells

Tools