Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells

Autoren

Andreas Giessl
Alexander Pulvermüller
Philipp Trojan
Jung Hee Park
Hui-Woog Choe
Oliver Peter Ernst
Klaus Peter Hofmann
Uwe Wolfrum

DOI

10.1074/jbc.m406770200

ISSN

0021-9258

Ausgabe der Veröffentlichung

49

Zeitschrift

Journal of Biological Chemistry

Sprache

en

Paginierung

51472 - 51481

Datum der Veröffentlichung

2004

Status

Published

Herausgeber

Elsevier BV

Herausgeber URL

http://dx.doi.org/10.1074/jbc.m406770200

Datum der Datenerfassung

2021

Titel

Differential Expression and Interaction with the Visual G-protein Transducin of Centrin Isoforms in Mammalian Photoreceptor Cells

Ausgabe der Zeitschrift

279

Data source: Crossref

Abstract

Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, Gt, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca(2+)-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to Gtbetagamma heterodimers. High affinity binding to Gtbetagamma and subcellular localization of the centrin isoforms Cen1 and Cen2 in the connecting cilium indicated that these isoforms contribute to the centrin-transducin complex and potentially participate in the regulation of transducin translocation through the photoreceptor cilium. Binding of Cen2 and Cen4 to Gbetagamma of non-visual G-proteins may additionally regulate G-proteins involved in centrosome and basal body functions.

Addresses

Institut für Zoologie, Johannes Gutenberg-Universität Mainz, 55099 Mainz, Germany.

Autoren

Andreas Giessl
Alexander Pulvermüller
Philipp Trojan
Jung Hee Park
Hui-Woog Choe
Oliver Peter Ernst
Klaus Peter Hofmann
Uwe Wolfrum

DOI

10.1074/jbc.m406770200

eISSN

1083-351X

Externe Identifier

PubMed Identifier: 15347651

Open access

false

ISSN

0021-9258

Ausgabe der Veröffentlichung

49

Zeitschrift

The Journal of biological chemistry

Schlüsselwörter

Retina
Cilia
Centrosome
Animals
Mice, Inbred C57BL
Cattle
Mice
Rats
Rats, Sprague-Dawley
Calcium
Transducin
Glutathione Transferase
Rhodopsin
Calcium-Binding Proteins
Protein Isoforms
Recombinant Proteins
Cyclic GMP
Microscopy, Immunoelectron
Microscopy, Fluorescence
Blotting, Western
Chromatography
Electrophoresis, Polyacrylamide Gel
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Protein Binding
Protein Transport
Dose-Response Relationship, Drug
Kinetics
Light
Scattering, Radiation
Rod Cell Outer Segment

Sprache

eng

Medium

Print-Electronic

Online publication date

2004

Paginierung

51472 - 51481

Datum der Veröffentlichung

2004

Status

Published

Publisher licence

CC BY

Datum der Datenerfassung

2004

Titel

Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

279

Data source: Europe PubMed Central

Abstract

Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, Gt, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca(2+)-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to Gtbetagamma heterodimers. High affinity binding to Gtbetagamma and subcellular localization of the centrin isoforms Cen1 and Cen2 in the connecting cilium indicated that these isoforms contribute to the centrin-transducin complex and potentially participate in the regulation of transducin translocation through the photoreceptor cilium. Binding of Cen2 and Cen4 to Gbetagamma of non-visual G-proteins may additionally regulate G-proteins involved in centrosome and basal body functions.

Autoren

Andreas Giessl
Alexander Pulvermüller
Philipp Trojan
Jung Hee Park
Hui-Woog Choe
Oliver Peter Ernst
Klaus Peter Hofmann
Uwe Wolfrum

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/15347651

DOI

10.1074/jbc.M406770200

ISSN

0021-9258

Ausgabe der Veröffentlichung

49

Zeitschrift

J Biol Chem

Schlüsselwörter

Animals
Blotting, Western
Calcium
Calcium-Binding Proteins
Cattle
Centrosome
Chromatography
Cilia
Cyclic GMP
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Glutathione Transferase
Kinetics
Light
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Microscopy, Immunoelectron
Protein Binding
Protein Isoforms
Protein Transport
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Retina
Reverse Transcriptase Polymerase Chain Reaction
Rhodopsin
Rod Cell Outer Segment
Scattering, Radiation
Sequence Analysis, DNA
Transducin

Sprache

eng

Country

United States

Paginierung

51472 - 51481

PII

S0021-9258(20)69442-X

Datum der Veröffentlichung

2004

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2005

Titel

Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

279

Data source: PubMed

Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells

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