Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP

Publication type:
Journal article
Metadata:
Autoren
  • Wilda Orisme
  • Jian Li
  • Tobias Goldmann
  • Susan Bolch
  • Uwe Wolfrum
  • W Clay Smith
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000273935700012&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.cellsig.2009.10.016
eISSN
1873-3913
Externe Identifier
  • Clarivate Analytics Document Solution ID: 548CD
  • PubMed Identifier: 19887106
ISSN
0898-6568
Ausgabe der Veröffentlichung
3
Zeitschrift
CELLULAR SIGNALLING
Schlüsselwörter
  • Arrestin
  • Translocation
  • G-protein
  • Phosphoinositides
  • Protein kinase C
  • Rod photoreceptors
Paginierung
447 - 456
Datum der Veröffentlichung
2010
Status
Published
Titel
Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP
Sub types
  • Article
Ausgabe der Zeitschrift
22

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Wilda Orisme
  • Jian Li
  • Tobias Goldmann
  • Susan Bolch
  • Uwe Wolfrum
  • W Clay Smith
DOI
10.1016/j.cellsig.2009.10.016
ISSN
0898-6568
Ausgabe der Veröffentlichung
3
Zeitschrift
Cellular Signalling
Sprache
en
Paginierung
447 - 456
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.cellsig.2009.10.016
Datum der Datenerfassung
2021
Titel
Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP
Ausgabe der Zeitschrift
22

Data source: Crossref

Abstract
Partitioning of cellular components is a critical mechanism by which cells can regulate their activity. In rod photoreceptors, light induces a large-scale translocation of arrestin from the inner segments to the outer segments. The purpose of this project is to elucidate the signaling pathway necessary to initiate arrestin translocation to the outer segments and the mechanism for arrestin translocation. Mouse retinal organotypic cultures and eyes from transgenic Xenopus tadpoles expressing a fusion of GFP and rod arrestin were treated with both activators and inhibitors of proteins in the phosphoinositide pathway. Confocal microscopy was used to image the effects of the pharmacological agents on arrestin translocation in rod photoreceptors. Retinas were also depleted of ATP using potassium cyanide to assess the requirement for ATP in arrestin translocation. In this study, we demonstrate that components of the G-protein-linked phospholipase C (PLC) pathway play a role in initiating arrestin translocation. Our results show that arrestin translocation can be stimulated by activators of PLC and protein kinase C (PKC), and by cholera toxin in the absence of light. Arrestin translocation to the outer segments is significantly reduced by inhibitors of PLC and PKC. Importantly, we find that treatment with potassium cyanide inhibits arrestin translocation in response to light. Collectively, our results suggest that arrestin translocation is initiated by a G-protein-coupled cascade through PLC and PKC signaling. Furthermore, our results demonstrate that at least the initiation of arrestin translocation requires energy input.
Addresses
  • Department of Ophthalmology, University of Florida, Gainesville, Florida 32610-0284, USA.
Autoren
  • Wilda Orisme
  • Jian Li
  • Tobias Goldmann
  • Tobias Goldmann
  • Susan Bolch
  • Uwe Wolfrum
  • W Clay Smith
DOI
10.1016/j.cellsig.2009.10.016
eISSN
1873-3913
Externe Identifier
  • PubMed Identifier: 19887106
  • PubMed Central ID: PMC2794968
Funding acknowledgements
  • NEI NIH HHS: EY08571
  • NEI NIH HHS: P30 EY021721
  • NEI NIH HHS: R01 EY014864
  • FAUN-Stiftung, Nürnberg, Germany:
  • National Eye Institute: EY014864
  • Deutsche Forschungsgemeinschaft: GRK1044
  • NEI NIH HHS: R01 EY006225
  • NEI NIH HHS: R01 EY006225-24
  • NEI NIH HHS: T32 EY007132-17
  • NEI NIH HHS: EY007132
  • NEI NIH HHS: EY014864
  • NEI NIH HHS: T32 EY007132
  • Research to Prevent Blindness:
  • NEI NIH HHS: P30 EY008571
  • National Eye Institute: EY06225
  • National Eye Institute: EY08571
  • NEI NIH HHS: P30 EY008571-20
  • NEI NIH HHS: R01 EY014864-05
  • Karl Kirchgessner Foundation:
  • National Eye Institute: EY007132
  • NEI NIH HHS: EY06225
Open access
false
ISSN
0898-6568
Ausgabe der Veröffentlichung
3
Zeitschrift
Cellular signalling
Schlüsselwörter
  • Cells, Cultured
  • Animals
  • Xenopus laevis
  • Mice
  • Potassium Cyanide
  • Cholera Toxin
  • Protein Kinase C
  • Phosphatidylinositols
  • Arrestin
  • Adenosine Triphosphate
  • Enzyme Inhibitors
  • Signal Transduction
  • Light
  • Type C Phospholipases
  • Retinal Rod Photoreceptor Cells
Sprache
eng
Medium
Print
Paginierung
447 - 456
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2009
Titel
Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP.
Sub types
  • Research Support, Non-U.S. Gov't
  • research-article
  • Journal Article
  • Research Support, N.I.H., Extramural
Ausgabe der Zeitschrift
22

Data source: Europe PubMed Central

Abstract
Partitioning of cellular components is a critical mechanism by which cells can regulate their activity. In rod photoreceptors, light induces a large-scale translocation of arrestin from the inner segments to the outer segments. The purpose of this project is to elucidate the signaling pathway necessary to initiate arrestin translocation to the outer segments and the mechanism for arrestin translocation. Mouse retinal organotypic cultures and eyes from transgenic Xenopus tadpoles expressing a fusion of GFP and rod arrestin were treated with both activators and inhibitors of proteins in the phosphoinositide pathway. Confocal microscopy was used to image the effects of the pharmacological agents on arrestin translocation in rod photoreceptors. Retinas were also depleted of ATP using potassium cyanide to assess the requirement for ATP in arrestin translocation. In this study, we demonstrate that components of the G-protein-linked phospholipase C (PLC) pathway play a role in initiating arrestin translocation. Our results show that arrestin translocation can be stimulated by activators of PLC and protein kinase C (PKC), and by cholera toxin in the absence of light. Arrestin translocation to the outer segments is significantly reduced by inhibitors of PLC and PKC. Importantly, we find that treatment with potassium cyanide inhibits arrestin translocation in response to light. Collectively, our results suggest that arrestin translocation is initiated by a G-protein-coupled cascade through PLC and PKC signaling. Furthermore, our results demonstrate that at least the initiation of arrestin translocation requires energy input.
Date of acceptance
2009
Autoren
  • Wilda Orisme
  • Jian Li
  • Tobias Goldmann
  • Susan Bolch
  • Uwe Wolfrum
  • W Clay Smith
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19887106
DOI
10.1016/j.cellsig.2009.10.016
eISSN
1873-3913
Externe Identifier
  • NIH Manuscript Submission ID: NIHMS156715
  • PubMed Central ID: PMC2794968
Funding acknowledgements
  • NEI NIH HHS: EY08571
  • NEI NIH HHS: EY014864
  • NEI NIH HHS: R01 EY014864
  • NEI NIH HHS: EY06225
  • NEI NIH HHS: R01 EY014864-05
  • NEI NIH HHS: R01 EY006225-24
  • NEI NIH HHS: T32 EY007132-17
  • NEI NIH HHS: T32 EY007132
  • NEI NIH HHS: P30 EY008571
  • NEI NIH HHS: P30 EY021721
  • NEI NIH HHS: P30 EY008571-20
  • NEI NIH HHS: EY007132
  • NEI NIH HHS: R01 EY006225
Ausgabe der Veröffentlichung
3
Zeitschrift
Cell Signal
Schlüsselwörter
  • Adenosine Triphosphate
  • Animals
  • Arrestin
  • Cells, Cultured
  • Cholera Toxin
  • Enzyme Inhibitors
  • Light
  • Mice
  • Phosphatidylinositols
  • Potassium Cyanide
  • Protein Kinase C
  • Retinal Rod Photoreceptor Cells
  • Signal Transduction
  • Type C Phospholipases
  • Xenopus laevis
Sprache
eng
Country
England
Paginierung
447 - 456
PII
S0898-6568(09)00333-7
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP.
Sub types
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
22

Data source: PubMed

Beziehungen:
Property of

Tools