The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton

Publication type:
Journal article
Metadata:
Autoren
  • Boris Reidel
  • Tobias Goldmann
  • Andreas Giessl
  • Uwe Wolfrum
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000259664000002&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1002/cm.20300
Externe Identifier
  • Clarivate Analytics Document Solution ID: 354UH
  • PubMed Identifier: 18623243
ISSN
0886-1544
Ausgabe der Veröffentlichung
10
Zeitschrift
CELL MOTILITY AND THE CYTOSKELETON
Schlüsselwörter
  • vision
  • arrestin
  • transducin
  • G-protein
  • fight adaptation
  • molecular movements
Paginierung
785 - 800
Datum der Veröffentlichung
2008
Status
Published
Titel
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton
Sub types
  • Article
Ausgabe der Zeitschrift
65

Data source: Web of Science (Lite)

Other metadata sources:
Abstract
<jats:title>Abstract</jats:title><jats:p>In vertebrate rod photoreceptor cells, arrestin and the visual G‐protein transducin move between the inner segment and outer segment in response to changes in light. This stimulus dependent translocation of signalling molecules is assumed to participate in long term light adaptation of photoreceptors. So far the cellular basis for the transport mechanisms underlying these intracellular movements remains largely elusive. Here we investigated the dependency of these movements on actin filaments and the microtubule cytoskeleton of photoreceptor cells. Co‐cultures of mouse retina and retinal pigment epithelium were incubated with drugs stabilizing and destabilizing the cytoskeleton. The actin and microtubule cytoskeleton and the light dependent distribution of signaling molecules were subsequently analyzed by light and electron microscopy. The application of cytoskeletal drugs differentially affected the cytoskeleton in photoreceptor compartments. During dark adaptation the depolymerization of microtubules as well as actin filaments disrupted the translocation of arrestin and transducin in rod photoreceptor cells. During light adaptation only the delivery of arrestin within the outer segment was impaired after destabilization of microtubules. Movements of transducin and arrestin required intact cytoskeletal elements in dark adapting cells. However, diffusion might be sufficient for the fast molecular movements observed as cells adapt to light. These findings indicate that different molecular translocation mechanisms are responsible for the dark and light associated translocations of arrestin and transducin in rod photoreceptor cells. Cell Motil. Cytoskeleton 65: 785–800, 2008. © 2008 Wiley‐Liss, Inc.</jats:p>
Autoren
  • Boris Reidel
  • Tobias Goldmann
  • Andreas Giessl
  • Uwe Wolfrum
DOI
10.1002/cm.20300
ISSN
0886-1544
Ausgabe der Veröffentlichung
10
Zeitschrift
Cell Motility
Sprache
en
Online publication date
2008
Paginierung
785 - 800
Datum der Veröffentlichung
2008
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1002/cm.20300
Datum der Datenerfassung
2023
Titel
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton
Ausgabe der Zeitschrift
65

Data source: Crossref

Abstract
In vertebrate rod photoreceptor cells, arrestin and the visual G-protein transducin move between the inner segment and outer segment in response to changes in light. This stimulus dependent translocation of signalling molecules is assumed to participate in long term light adaptation of photoreceptors. So far the cellular basis for the transport mechanisms underlying these intracellular movements remains largely elusive. Here we investigated the dependency of these movements on actin filaments and the microtubule cytoskeleton of photoreceptor cells. Co-cultures of mouse retina and retinal pigment epithelium were incubated with drugs stabilizing and destabilizing the cytoskeleton. The actin and microtubule cytoskeleton and the light dependent distribution of signaling molecules were subsequently analyzed by light and electron microscopy. The application of cytoskeletal drugs differentially affected the cytoskeleton in photoreceptor compartments. During dark adaptation the depolymerization of microtubules as well as actin filaments disrupted the translocation of arrestin and transducin in rod photoreceptor cells. During light adaptation only the delivery of arrestin within the outer segment was impaired after destabilization of microtubules. Movements of transducin and arrestin required intact cytoskeletal elements in dark adapting cells. However, diffusion might be sufficient for the fast molecular movements observed as cells adapt to light. These findings indicate that different molecular translocation mechanisms are responsible for the dark and light associated translocations of arrestin and transducin in rod photoreceptor cells.
Addresses
  • Department of Cell and Matrix Biology, Institute of Zoology, Johannes Gutenberg University of Mainz, Germany.
Autoren
  • Boris Reidel
  • Tobias Goldmann
  • Andreas Giessl
  • Uwe Wolfrum
DOI
10.1002/cm.20300
eISSN
1097-0169
Externe Identifier
  • PubMed Identifier: 18623243
Open access
false
ISSN
0886-1544
Ausgabe der Veröffentlichung
10
Zeitschrift
Cell motility and the cytoskeleton
Schlüsselwörter
  • Retina
  • Cytoskeleton
  • Microtubules
  • Animals
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice
  • Paclitaxel
  • Thiabendazole
  • Cytochalasin D
  • Phalloidine
  • Actins
  • Transducin
  • Arrestin
  • Microscopy, Immunoelectron
  • Microscopy, Fluorescence
  • Signal Transduction
  • Protein Transport
  • Dark Adaptation
  • Homozygote
  • Light
  • Darkness
  • Cell Migration Assays
  • Rod Cell Outer Segment
  • Vision, Ocular
  • Retinal Rod Photoreceptor Cells
  • Actin Cytoskeleton
  • Heterocyclic Compounds, 4 or More Rings
Sprache
eng
Medium
Print
Paginierung
785 - 800
Datum der Veröffentlichung
2008
Status
Published
Datum der Datenerfassung
2008
Titel
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
65

Data source: Europe PubMed Central

Abstract
In vertebrate rod photoreceptor cells, arrestin and the visual G-protein transducin move between the inner segment and outer segment in response to changes in light. This stimulus dependent translocation of signalling molecules is assumed to participate in long term light adaptation of photoreceptors. So far the cellular basis for the transport mechanisms underlying these intracellular movements remains largely elusive. Here we investigated the dependency of these movements on actin filaments and the microtubule cytoskeleton of photoreceptor cells. Co-cultures of mouse retina and retinal pigment epithelium were incubated with drugs stabilizing and destabilizing the cytoskeleton. The actin and microtubule cytoskeleton and the light dependent distribution of signaling molecules were subsequently analyzed by light and electron microscopy. The application of cytoskeletal drugs differentially affected the cytoskeleton in photoreceptor compartments. During dark adaptation the depolymerization of microtubules as well as actin filaments disrupted the translocation of arrestin and transducin in rod photoreceptor cells. During light adaptation only the delivery of arrestin within the outer segment was impaired after destabilization of microtubules. Movements of transducin and arrestin required intact cytoskeletal elements in dark adapting cells. However, diffusion might be sufficient for the fast molecular movements observed as cells adapt to light. These findings indicate that different molecular translocation mechanisms are responsible for the dark and light associated translocations of arrestin and transducin in rod photoreceptor cells.
Autoren
  • Boris Reidel
  • Tobias Goldmann
  • Andreas Giessl
  • Uwe Wolfrum
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/18623243
DOI
10.1002/cm.20300
eISSN
1097-0169
Ausgabe der Veröffentlichung
10
Zeitschrift
Cell Motil Cytoskeleton
Schlüsselwörter
  • Actin Cytoskeleton
  • Actins
  • Animals
  • Arrestin
  • Cell Migration Assays
  • Cytochalasin D
  • Cytoskeleton
  • Dark Adaptation
  • Darkness
  • Heterocyclic Compounds, 4 or More Rings
  • Homozygote
  • Light
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Fluorescence
  • Microscopy, Immunoelectron
  • Microtubules
  • Paclitaxel
  • Phalloidine
  • Protein Transport
  • Retina
  • Retinal Rod Photoreceptor Cells
  • Rod Cell Outer Segment
  • Signal Transduction
  • Thiabendazole
  • Transducin
  • Vision, Ocular
Sprache
eng
Country
United States
Paginierung
785 - 800
Datum der Veröffentlichung
2008
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2008
Titel
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
65

Data source: PubMed

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