A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo

Publication type:
Chapter
Metadata:
Autoren
  • Kirsten A Wunderlich
  • Uwe Wolfrum
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000399047000009&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1007/978-1-4939-3789-9_7
ISBN-13
978-1-4939-3787-5
Schlüsselwörter
  • Ciliary transport
  • Primary sensory cilia
  • Ciliated photoreceptor cells
  • Rhodopsin
  • Intracellular transport
Paginierung
97 - 106
Buchtitel
CILIA: METHODS AND PROTOCOLS
Datum der Veröffentlichung
2016
Status
Published
Titel
A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo
Ausgabe der Zeitschrift
1454

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Kirsten A Wunderlich
  • Uwe Wolfrum
DOI
10.1007/978-1-4939-3789-9_7
ISBN-13
9781493937875
Online publication date
2016
Paginierung
97 - 106
Buchtitel
Methods in Molecular Biology
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Springer New York
Herausgeber URL
http://dx.doi.org/10.1007/978-1-4939-3789-9_7
Datum der Datenerfassung
2017
Titel
A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo

Data source: Crossref

Abstract
The outer segment of rod and cone photoreceptor cells represents a highly modified primary sensory cilium. It renews on a daily basis throughout lifetime and effective vectorial transport to the cilium is essential for the maintenance of the photoreceptor cell function. Defects in molecules of transport modules lead to severe retinal ciliopathies. We have recently established a fluorescence recovery after photobleaching (FRAP)-based method to monitor molecular trafficking in living rodent photoreceptor cells. We irreversibly bleach the fluorescence of tagged molecules (e.g. eGFP-Rhodopsin) in photoreceptor cells of native vibratome sections through the retina by high laser intensity. In the laser scanning microscope, the recovery of the fluorescent signal is monitored over time and the kinetics of movements of molecules can be quantitatively ascertained.
Addresses
  • Institute of Zoology, Cell and Matrix Biology, Johannes Gutenberg University of Mainz, Müllerweg 6, 55099, Mainz, Germany.
Autoren
  • Kirsten A Wunderlich
  • Uwe Wolfrum
DOI
10.1007/978-1-4939-3789-9_7
Open access
false
Schlüsselwörter
  • Cilia
  • Animals
  • Mice, Transgenic
  • Mice
  • Rhodopsin
  • Fluorescence Recovery After Photobleaching
  • Gene Expression
  • Biological Transport
  • Protein Transport
  • Genes, Reporter
  • Photoreceptor Cells
  • Rod Cell Outer Segment
Medium
Print
Paginierung
97 - 106
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.
Ausgabe der Zeitschrift
1454

Data source: Europe PubMed Central

Abstract
The outer segment of rod and cone photoreceptor cells represents a highly modified primary sensory cilium. It renews on a daily basis throughout lifetime and effective vectorial transport to the cilium is essential for the maintenance of the photoreceptor cell function. Defects in molecules of transport modules lead to severe retinal ciliopathies. We have recently established a fluorescence recovery after photobleaching (FRAP)-based method to monitor molecular trafficking in living rodent photoreceptor cells. We irreversibly bleach the fluorescence of tagged molecules (e.g. eGFP-Rhodopsin) in photoreceptor cells of native vibratome sections through the retina by high laser intensity. In the laser scanning microscope, the recovery of the fluorescent signal is monitored over time and the kinetics of movements of molecules can be quantitatively ascertained.
Autoren
  • Kirsten A Wunderlich
  • Uwe Wolfrum
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/27514918
DOI
10.1007/978-1-4939-3789-9_7
Schlüsselwörter
  • Ciliary transport
  • Ciliated photoreceptor cells
  • Intracellular transport
  • Primary sensory cilia
  • Rhodopsin
  • Animals
  • Biological Transport
  • Cilia
  • Fluorescence Recovery After Photobleaching
  • Gene Expression
  • Genes, Reporter
  • Mice
  • Mice, Transgenic
  • Photoreceptor Cells
  • Protein Transport
  • Rhodopsin
  • Rod Cell Outer Segment
Paginierung
97 - 106
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2018
Titel
A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.
Ausgabe der Zeitschrift
1454

Data source: PubMed

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