The Rat1p 5’ to 3’ exonuclease degrades telomeric repeat-containing RNA and promotes telomere elongation in Saccharomyces cerevisiae

Publication type:
Journal article
Metadata:
Autoren
  • Brian Luke
  • Andrea Panza
  • Sophie Redon
  • Nahid Iglesias
  • Zhijian Li
  • Joachim Lingner
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000261290000005&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.molcel.2008.10.019
eISSN
1097-4164
Externe Identifier
  • Clarivate Analytics Document Solution ID: 377ZD
  • PubMed Identifier: 19026778
ISSN
1097-2765
Ausgabe der Veröffentlichung
4
Zeitschrift
MOLECULAR CELL
Paginierung
465 - 477
Datum der Veröffentlichung
2008
Status
Published
Titel
The Rat1p 5′ to 3′ Exonuclease Degrades Telomeric Repeat-Containing RNA and Promotes Telomere Elongation in <i>Saccharomyces cerevisiae</i>
Sub types
  • Article
Ausgabe der Zeitschrift
32

Data source: Web of Science (Lite)

Other metadata sources:
Autoren
  • Brian Luke
  • Andrea Panza
  • Sophie Redon
  • Nahid Iglesias
  • Zhijian Li
  • Joachim Lingner
DOI
10.1016/j.molcel.2008.10.019
ISSN
1097-2765
Ausgabe der Veröffentlichung
4
Zeitschrift
Molecular Cell
Sprache
en
Paginierung
465 - 477
Datum der Veröffentlichung
2008
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.molcel.2008.10.019
Datum der Datenerfassung
2024
Titel
The Rat1p 5′ to 3′ Exonuclease Degrades Telomeric Repeat-Containing RNA and Promotes Telomere Elongation in Saccharomyces cerevisiae
Ausgabe der Zeitschrift
32

Data source: Crossref

Abstract
Vertebrate telomeres are transcribed into telomeric repeat-containing RNA (TERRA) that associates with telomeres and may be important for telomere function. Here, we demonstrate that telomeres are also transcribed in Saccharomyces cerevisiae by RNA polymerase II (RNAPII). Yeast TERRA is polyadenylated and stabilized by Pap1p and regulated by the 5' to 3' exonuclease, Rat1p. rat1-1 mutant cells accumulate TERRA and harbor short telomeres because of defects in telomerase-mediated telomere elongation. Overexpression of RNaseH overcomes telomere elongation defects in rat1-1 cells, indicating that RNA/DNA hybrids inhibit telomerase function at chromosome ends in these mutants. Thus, telomeric transcription combined with Rat1p-dependent TERRA degradation is important for regulating telomerase in yeast. Telomere transcription is conserved in different kingdoms of the eukaryotic domain.
Addresses
  • Ecole Polytechnique Fédérale de Lausanne, Swiss Institute for Experimental Cancer Research (ISREC), CH-1066 Epalinges, Switzerland.
Autoren
  • Brian Luke
  • Andrea Panza
  • Sophie Redon
  • Nahid Iglesias
  • Zhijian Li
  • Joachim Lingner
DOI
10.1016/j.molcel.2008.10.019
eISSN
1097-4164
Externe Identifier
  • PubMed Identifier: 19026778
Open access
false
ISSN
1097-2765
Ausgabe der Veröffentlichung
4
Zeitschrift
Molecular cell
Schlüsselwörter
  • Telomere
  • Saccharomyces cerevisiae
  • Exonucleases
  • Exoribonucleases
  • Telomeric Repeat Binding Protein 1
  • Saccharomyces cerevisiae Proteins
  • RNA
  • RNA, Fungal
  • Models, Biological
Sprache
eng
Medium
Print
Paginierung
465 - 477
Datum der Veröffentlichung
2008
Status
Published
Datum der Datenerfassung
2008
Titel
The Rat1p 5' to 3' exonuclease degrades telomeric repeat-containing RNA and promotes telomere elongation in Saccharomyces cerevisiae.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
32

Data source: Europe PubMed Central

Abstract
Vertebrate telomeres are transcribed into telomeric repeat-containing RNA (TERRA) that associates with telomeres and may be important for telomere function. Here, we demonstrate that telomeres are also transcribed in Saccharomyces cerevisiae by RNA polymerase II (RNAPII). Yeast TERRA is polyadenylated and stabilized by Pap1p and regulated by the 5' to 3' exonuclease, Rat1p. rat1-1 mutant cells accumulate TERRA and harbor short telomeres because of defects in telomerase-mediated telomere elongation. Overexpression of RNaseH overcomes telomere elongation defects in rat1-1 cells, indicating that RNA/DNA hybrids inhibit telomerase function at chromosome ends in these mutants. Thus, telomeric transcription combined with Rat1p-dependent TERRA degradation is important for regulating telomerase in yeast. Telomere transcription is conserved in different kingdoms of the eukaryotic domain.
Date of acceptance
2008
Autoren
  • Brian Luke
  • Andrea Panza
  • Sophie Redon
  • Nahid Iglesias
  • Zhijian Li
  • Joachim Lingner
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/19026778
DOI
10.1016/j.molcel.2008.10.019
eISSN
1097-4164
Ausgabe der Veröffentlichung
4
Zeitschrift
Mol Cell
Schlüsselwörter
  • Exonucleases
  • Exoribonucleases
  • Models, Biological
  • RNA
  • RNA, Fungal
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Telomere
  • Telomeric Repeat Binding Protein 1
Sprache
eng
Country
United States
Paginierung
465 - 477
PII
S1097-2765(08)00759-4
Datum der Veröffentlichung
2008
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2009
Titel
The Rat1p 5' to 3' exonuclease degrades telomeric repeat-containing RNA and promotes telomere elongation in Saccharomyces cerevisiae.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
32

Data source: PubMed

Abstract
Vertebrate telomeres are transcribed into telomeric repeat-containing RNA (TERRA) that associates with telomeres and may be important for telomere function. Here, we demonstrate that telomeres are also transcribed in Saccharomyces cerevisiae by RNA polymerase II (RNAPII). Yeast TERRA is polyadenylated and stabilized by Pap1p and regulated by the 5’ to 3’ exonuclease, Rat1p. rat1-1 mutant cells accumulate TERRA and harbor short telomeres because of defects in telomerase-mediated telomere elongation. Overexpression of RNaseH overcomes telomere elongation defects in rat1-1 cells, indicating that RNA/DNA hybrids inhibit telomerase function at chromosome ends in these mutants. Thus, telomeric transcription combined with Rat1p-dependent TERRA degradation is important for regulating telomerase in yeast. Telomere transcription is conserved in different kingdoms of the eukaryotic domain.
Autoren
  • Brian Luke
  • Andrea Panza
  • Sophie Redon
  • Nahid Iglesias
  • Zhijian Li
  • Joachim Lingner
DOI
10.1016/j.molcel.2008.10.019
Zeitschrift
Molecular cell
Notes
keywords: Exonucleases/genetics/metabolism;Exoribonucleases/genetics/metabolism;Models, Biological;RNA, Fungal/genetics;RNA/genetics/metabolism;Saccharomyces cerevisiae Proteins/genetics/metabolism;Saccharomyces cerevisiae/genetics/metabolism;Telomere/metabolism;Telomeric Repeat Binding Protein 1/genetics/metabolism
Artikelnummer
4
Paginierung
465 - 477
Datum der Veröffentlichung
2008
Datum der Datenerfassung
2023
Titel
The Rat1p 5’ to 3’ exonuclease degrades telomeric repeat-containing RNA and promotes telomere elongation in Saccharomyces cerevisiae
Sub types
  • article
Ausgabe der Zeitschrift
32

Data source: Manual

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