Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Baran E Gueler
- Joshua Linnert
- Uwe Wolfrum
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000965483200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/bcpt.13860
- eISSN
- 1742-7843
- Externe Identifier
- Clarivate Analytics Document Solution ID: U2AP0
- PubMed Identifier: 36929698
- ISSN
- 1742-7835
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
- Schlüsselwörter
- aGPCR
- cell migration
- epilepsy
- focal adhesions
- G protein-coupled receptors
- mechanoreception
- Usher syndrome
- Paginierung
- 301 - 312
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Titel
- Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly
- Sub types
- Article
- Ausgabe der Zeitschrift
- 133
Data source: Web of Science (Lite)
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>VLGR1/ADGRV1 (very large G protein‐coupled receptor‐1) is the largest adhesion G protein‐coupled receptor (aGPCR). Mutations in <jats:italic>VLGR1/ADGRV1</jats:italic> are associated with human Usher syndrome, the most common form of deaf‐blindness, and also with epilepsy in humans and mice. VLGR1 is expressed almost ubiquitously but is mainly found in the CNS and in the sensory cells of the eye and inner ear. Little is known about the pathogenesis of the diseases related to VLGR1. We previously identified VLGR1 as a vital component of focal adhesions (FAs) serving as a metabotropic mechanoreceptor controls cell spreading and migration. FAs are highly dynamic and turnover in response to internal and external signals. Here, we aimed to elucidate how VLGR1 participates in FA turnover. Nocodazole washouts and live cell imaging of paxillin‐DsRed2 consistently showed that FA disassembly was not altered, but de novo assembly of FA was significantly delayed in <jats:italic>Vlgr1</jats:italic>‐deficient astrocytes, indicating that VLGR1 is enrolled in FA assembly. In FRAP experiments, recovery rates were significantly reduced in <jats:italic>Vlgr1</jats:italic>‐deficient FAs, indicating reduced turnover kinetics in VLGR1‐deficient FAs. We showed that VLGR1 regulates cell migration by controlling the FA turnover during their assembly and expect novel insights into pathomechanisms related to pathogenic dysfunctions of VLGR1.</jats:p>
- Autoren
- Baran E Güler
- Joshua Linnert
- Uwe Wolfrum
- DOI
- 10.1111/bcpt.13860
- eISSN
- 1742-7843
- ISSN
- 1742-7835
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Basic & Clinical Pharmacology & Toxicology
- Sprache
- en
- Online publication date
- 2023
- Paginierung
- 301 - 312
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/bcpt.13860
- Datum der Datenerfassung
- 2023
- Titel
- Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly
- Ausgabe der Zeitschrift
- 133
Data source: Crossref
- Abstract
- VLGR1/ADGRV1 (very large G protein-coupled receptor-1) is the largest adhesion G protein-coupled receptor (aGPCR). Mutations in VLGR1/ADGRV1 are associated with human Usher syndrome, the most common form of deaf-blindness, and also with epilepsy in humans and mice. VLGR1 is expressed almost ubiquitously but is mainly found in the CNS and in the sensory cells of the eye and inner ear. Little is known about the pathogenesis of the diseases related to VLGR1. We previously identified VLGR1 as a vital component of focal adhesions (FAs) serving as a metabotropic mechanoreceptor controls cell spreading and migration. FAs are highly dynamic and turnover in response to internal and external signals. Here, we aimed to elucidate how VLGR1 participates in FA turnover. Nocodazole washouts and live cell imaging of paxillin-DsRed2 consistently showed that FA disassembly was not altered, but de novo assembly of FA was significantly delayed in Vlgr1-deficient astrocytes, indicating that VLGR1 is enrolled in FA assembly. In FRAP experiments, recovery rates were significantly reduced in Vlgr1-deficient FAs, indicating reduced turnover kinetics in VLGR1-deficient FAs. We showed that VLGR1 regulates cell migration by controlling the FA turnover during their assembly and expect novel insights into pathomechanisms related to pathogenic dysfunctions of VLGR1.
- Addresses
- Institute of Molecular Physiology, Molecular Cell Biology, Johannes Gutenberg University Mainz, Mainz, Germany.
- Autoren
- Baran E Güler
- Joshua Linnert
- Uwe Wolfrum
- DOI
- 10.1111/bcpt.13860
- eISSN
- 1742-7843
- Externe Identifier
- PubMed Identifier: 36929698
- Funding acknowledgements
- Deutsche Forschungsgemeinschaft: 246212759
- Deutsche Forschungsgemeinschaft: FOR 2149
- Foundation Fighting Blindness: PPA-0717-0719-RAD
- Foundation Fighting Blindness: PPA‐0717‐0719‐RAD
- Open access
- false
- ISSN
- 1742-7835
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Basic & clinical pharmacology & toxicology
- Schlüsselwörter
- Astrocytes
- Focal Adhesions
- Animals
- Humans
- Mice
- Receptors, G-Protein-Coupled
- Cell Movement
- Paxillin
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2023
- Paginierung
- 301 - 312
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Publisher licence
- CC BY-NC-ND
- Datum der Datenerfassung
- 2023
- Titel
- Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 133
Data source: Europe PubMed Central
- Abstract
- VLGR1/ADGRV1 (very large G protein-coupled receptor-1) is the largest adhesion G protein-coupled receptor (aGPCR). Mutations in VLGR1/ADGRV1 are associated with human Usher syndrome, the most common form of deaf-blindness, and also with epilepsy in humans and mice. VLGR1 is expressed almost ubiquitously but is mainly found in the CNS and in the sensory cells of the eye and inner ear. Little is known about the pathogenesis of the diseases related to VLGR1. We previously identified VLGR1 as a vital component of focal adhesions (FAs) serving as a metabotropic mechanoreceptor controls cell spreading and migration. FAs are highly dynamic and turnover in response to internal and external signals. Here, we aimed to elucidate how VLGR1 participates in FA turnover. Nocodazole washouts and live cell imaging of paxillin-DsRed2 consistently showed that FA disassembly was not altered, but de novo assembly of FA was significantly delayed in Vlgr1-deficient astrocytes, indicating that VLGR1 is enrolled in FA assembly. In FRAP experiments, recovery rates were significantly reduced in Vlgr1-deficient FAs, indicating reduced turnover kinetics in VLGR1-deficient FAs. We showed that VLGR1 regulates cell migration by controlling the FA turnover during their assembly and expect novel insights into pathomechanisms related to pathogenic dysfunctions of VLGR1.
- Date of acceptance
- 2023
- Autoren
- Baran E Güler
- Joshua Linnert
- Uwe Wolfrum
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/36929698
- DOI
- 10.1111/bcpt.13860
- eISSN
- 1742-7843
- Funding acknowledgements
- Foundation Fighting Blindness: PPA-0717-0719-RAD
- Ausgabe der Veröffentlichung
- 4
- Zeitschrift
- Basic Clin Pharmacol Toxicol
- Schlüsselwörter
- G protein-coupled receptors
- Usher syndrome
- aGPCR
- cell migration
- epilepsy
- focal adhesions
- mechanoreception
- Animals
- Humans
- Mice
- Paxillin
- Focal Adhesions
- Astrocytes
- Receptors, G-Protein-Coupled
- Cell Movement
- Sprache
- eng
- Country
- England
- Paginierung
- 301 - 312
- Datum der Veröffentlichung
- 2023
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Titel
- Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 133
Data source: PubMed
- Author's licence
- CC-BY-NC-ND
- Autoren
- Baran E Güler
- Joshua Linnert
- Uwe Wolfrum
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-491381577-H
- Resource version
- Published version
- DOI
- 10.1111/bcpt.13860
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1742-7835
- Zeitschrift
- Basic & clinical pharmacology & toxicology
- Schlüsselwörter
- 570 Biowissenschaften
- 570 Life sciences
- Sprache
- eng
- Open access status
- Open Access
- Datum der Veröffentlichung
- 2023
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/9241
- Herausgeber
- Wiley-Blackwell
- Datum der Datenerfassung
- 2023
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2023
- Zugang
- Public
- Titel
- Monitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assembly
- Ausgabe der Zeitschrift
- Version of Record (VoR)
Files
monitoring_paxillin_in_astroc-20230607100138313.pdf
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