Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Benjamin Wiench
- Tolga Eichhorn
- Bernhard Korn
- Malte Paulsen
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000308852400014&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1016/j.ymeth.2012.01.007
- eISSN
- 1095-9130
- Externe Identifier
- Clarivate Analytics Document Solution ID: 006XF
- PubMed Identifier: 22326879
- ISSN
- 1046-2023
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- METHODS
- Schlüsselwörter
- Inherent fluorescence
- Drug-uptake
- FACS
- Cytotoxicity
- High-throughput
- Real-time
- Paginierung
- 376 - 382
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Titel
- Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics
- Sub types
- Article
- Ausgabe der Zeitschrift
- 57
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Benjamin Wiench
- Tolga Eichhorn
- Bernhard Korn
- Malte Paulsen
- Thomas Efferth
- DOI
- 10.1016/j.ymeth.2012.01.007
- ISSN
- 1046-2023
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Methods
- Sprache
- en
- Paginierung
- 376 - 382
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Herausgeber
- Elsevier BV
- Herausgeber URL
- http://dx.doi.org/10.1016/j.ymeth.2012.01.007
- Datum der Datenerfassung
- 2023
- Titel
- Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics
- Ausgabe der Zeitschrift
- 57
Datenquelle: Crossref
- Abstract
- The precise detection of pharmaceutical drug uptake and knowledge of a drug's efficacy at the single-cell level is crucial for understanding a compound's performance. Many pharmaceutical drugs, like the model substances Doxorubicin, Mitoxantrone or Irinotecan, have a distinctive natural fluorescence that can be readily exploited for research purposes. Utilizing this respective natural fluorescence, we propose a method analyzing simultaneously in real-time the efficiency, effects and the associated kinetics of compound-uptake and efflux in mammalian cells by flow cytometry. We show that real-time flow cytometric quantification of compound-uptake is reliably measured and that analyzing their respective uptake kinetic provides additional valuable information which can be used for improving drug dosage and delivery. Exploiting the native fluorescence of natural compounds is clearly advantageous compared to the usage of non-related fluorescent uptake-reporter substances, possibly yielding in unphysiological data. Flow cytometric analysis allows live-dye based multi-parametric high-throughput screening of pharmaceutical compound activity, improving cytotoxicity testing by combining several assays into a single, high resolution readout. This approach can be a useful tool identifying potential inhibitors for multiple drug resistance (MDR), representing a major challenge to the targeted treatment of various diseases.
- Addresses
- Institute of Pharmacy and Biochemistry, Department of Pharmaceutical Biology, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
- Autoren
- Benjamin Wiench
- Tolga Eichhorn
- Bernhard Korn
- Malte Paulsen
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1016/j.ymeth.2012.01.007
- eISSN
- 1095-9130
- Externe Identifier
- PubMed Identifier: 22326879
- Open access
- false
- ISSN
- 1046-2023
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Methods (San Diego, Calif.)
- Schlüsselwörter
- Cell Line, Tumor
- Humans
- Reactive Oxygen Species
- Mitoxantrone
- Camptothecin
- Fluoresceins
- Doxorubicin
- Antineoplastic Agents
- Flow Cytometry
- Drug Resistance, Multiple
- Cell Survival
- Biological Transport
- Drug Resistance, Neoplasm
- Kinetics
- Fluorescence
- Single-Cell Analysis
- Irinotecan
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2012
- Paginierung
- 376 - 382
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum der Datenerfassung
- 2012
- Titel
- Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 57
Datenquelle: Europe PubMed Central
- Abstract
- The precise detection of pharmaceutical drug uptake and knowledge of a drug's efficacy at the single-cell level is crucial for understanding a compound's performance. Many pharmaceutical drugs, like the model substances Doxorubicin, Mitoxantrone or Irinotecan, have a distinctive natural fluorescence that can be readily exploited for research purposes. Utilizing this respective natural fluorescence, we propose a method analyzing simultaneously in real-time the efficiency, effects and the associated kinetics of compound-uptake and efflux in mammalian cells by flow cytometry. We show that real-time flow cytometric quantification of compound-uptake is reliably measured and that analyzing their respective uptake kinetic provides additional valuable information which can be used for improving drug dosage and delivery. Exploiting the native fluorescence of natural compounds is clearly advantageous compared to the usage of non-related fluorescent uptake-reporter substances, possibly yielding in unphysiological data. Flow cytometric analysis allows live-dye based multi-parametric high-throughput screening of pharmaceutical compound activity, improving cytotoxicity testing by combining several assays into a single, high resolution readout. This approach can be a useful tool identifying potential inhibitors for multiple drug resistance (MDR), representing a major challenge to the targeted treatment of various diseases.
- Date of acceptance
- 2012
- Autoren
- Benjamin Wiench
- Tolga Eichhorn
- Bernhard Korn
- Malte Paulsen
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/22326879
- DOI
- 10.1016/j.ymeth.2012.01.007
- eISSN
- 1095-9130
- Ausgabe der Veröffentlichung
- 3
- Zeitschrift
- Methods
- Schlüsselwörter
- Antineoplastic Agents
- Biological Transport
- Camptothecin
- Cell Line, Tumor
- Cell Survival
- Doxorubicin
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Flow Cytometry
- Fluoresceins
- Fluorescence
- Humans
- Irinotecan
- Kinetics
- Mitoxantrone
- Reactive Oxygen Species
- Single-Cell Analysis
- Sprache
- eng
- Country
- United States
- Paginierung
- 376 - 382
- PII
- S1046-2023(12)00023-0
- Datum der Veröffentlichung
- 2012
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2013
- Titel
- Utilizing inherent fluorescence of therapeutics to analyze real-time uptake and multi-parametric effector kinetics.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 57
Datenquelle: PubMed
- Beziehungen:
- Eigentum von