Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-κB pathway in chondrocytes
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Li Yan
- Li Zhou
- Danting Xie
- Wenxi Du
- Fangming Chen
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000468332900019&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.18632/aging.101952
- Externe Identifier
- Clarivate Analytics Document Solution ID: HY7RE
- PubMed Identifier: 31089001
- ISSN
- 1945-4589
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- AGING-US
- Schlüsselwörter
- platelet lysate
- growth factor
- osteoarthritis
- TNF-alpha
- NF-kappa B
- Paginierung
- 2797 - 2811
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Titel
- Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-κB pathway in chondrocytes
- Sub types
- Article
- Ausgabe der Zeitschrift
- 11
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Li Yan
- Li Zhou
- Danting Xie
- Wenxi Du
- Fangming Chen
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.18632/aging.101952
- eISSN
- 1945-4589
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Aging
- Sprache
- en
- Online publication date
- 2019
- Paginierung
- 2797 - 2811
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Herausgeber
- Impact Journals, LLC
- Herausgeber URL
- http://dx.doi.org/10.18632/aging.101952
- Datum der Datenerfassung
- 2020
- Titel
- Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes
- Ausgabe der Zeitschrift
- 11
Datenquelle: Crossref
- Abstract
- Platelet lysate (PL) contains a cocktail of growth factors that actively participates in cartilage repair. This study was designed to determine the effect and mechanism of PL on osteoarthritis (OA). An arthritis model was established to mimic human OA by intra-articular injection of monoiodoacetate (MIA) to Sprague Dawley (SD) rats. The model was weekly treated with PL by intra-articular injection. Thermal withdrawal latency, mechanical withdrawal threshold, and treadmill gait were tested for pain behavior observation. Histopathological and immunohistochemical analyses were conducted for evaluating cartilage degradation. Real time PCRs and Western blots were conducted to elucidate the mechanism of PL on primary chondrocytes. Results showed that, <i>in vivo</i>, PL significantly attenuated pain symptoms and exerted chondrocyte-protective and extracellular matrix (ECM)-modifying effect on the arthritic cartilage in a dose-dependent manner. The <i>in situ</i> expressions of type II Collagen (Col2) and matrix metalloproteinase 13 (Mmp13) in the arthritic cartilage was abnormal and was restored by PL. <i>In vitro</i>, PL significantly restored tumor necrosis factor α (TNF-α)-suppressed anabolic gene expression (<i>Col2</i> and <i>aggrecan</i>) and TNF-α-increased catabolic gene expression (<i>Col10</i>, <i>Mmp13</i>, <i>Adamts5</i>, and <i>Adamts9</i>) in chondrocytes. The effects were mediated by TNF-α downstream signaling, including inhibition of NF-κB and c-Jun activities. This study provides certain knowledge of anti-OA effect and TNF signaling-related mechanism of PL, placing it as a promising and alternative option for OA therapy in the future.
- Addresses
- The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
- Autoren
- Li Yan
- Li Zhou
- Danting Xie
- Wenxi Du
- Fangming Chen
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- DOI
- 10.18632/aging.101952
- eISSN
- 1945-4589
- Externe Identifier
- PubMed Identifier: 31089001
- PubMed Central ID: PMC6535074
- Open access
- true
- ISSN
- 1945-4589
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Aging
- Schlüsselwörter
- Blood Platelets
- Chondrocytes
- Animals
- Humans
- Rats
- Rats, Sprague-Dawley
- Arthritis
- Osteoarthritis
- Pain
- Iodoacetic Acid
- Tumor Necrosis Factor-alpha
- NF-kappa B
- Analgesics
- Random Allocation
- Cell Survival
- Gene Expression Regulation
- Male
- Sprache
- eng
- Medium
- Open access status
- Open Access
- Paginierung
- 2797 - 2811
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2019
- Titel
- Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes.
- Sub types
- Research Support, Non-U.S. Gov't
- research-article
- Journal Article
- Ausgabe der Zeitschrift
- 11
Files
https://europepmc.org/articles/PMC6535074?pdf=render
Datenquelle: Europe PubMed Central
- Abstract
- Platelet lysate (PL) contains a cocktail of growth factors that actively participates in cartilage repair. This study was designed to determine the effect and mechanism of PL on osteoarthritis (OA). An arthritis model was established to mimic human OA by intra-articular injection of monoiodoacetate (MIA) to Sprague Dawley (SD) rats. The model was weekly treated with PL by intra-articular injection. Thermal withdrawal latency, mechanical withdrawal threshold, and treadmill gait were tested for pain behavior observation. Histopathological and immunohistochemical analyses were conducted for evaluating cartilage degradation. Real time PCRs and Western blots were conducted to elucidate the mechanism of PL on primary chondrocytes. Results showed that, in vivo, PL significantly attenuated pain symptoms and exerted chondrocyte-protective and extracellular matrix (ECM)-modifying effect on the arthritic cartilage in a dose-dependent manner. The in situ expressions of type II Collagen (Col2) and matrix metalloproteinase 13 (Mmp13) in the arthritic cartilage was abnormal and was restored by PL. In vitro, PL significantly restored tumor necrosis factor α (TNF-α)-suppressed anabolic gene expression (Col2 and aggrecan) and TNF-α-increased catabolic gene expression (Col10, Mmp13, Adamts5, and Adamts9) in chondrocytes. The effects were mediated by TNF-α downstream signaling, including inhibition of NF-κB and c-Jun activities. This study provides certain knowledge of anti-OA effect and TNF signaling-related mechanism of PL, placing it as a promising and alternative option for OA therapy in the future.
- Date of acceptance
- 2019
- Autoren
- Li Yan
- Li Zhou
- Danting Xie
- Wenxi Du
- Fangming Chen
- Qiang Yuan
- Peijian Tong
- Letian Shan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/31089001
- DOI
- 10.18632/aging.101952
- eISSN
- 1945-4589
- Externe Identifier
- PubMed Central ID: PMC6535074
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Aging (Albany NY)
- Schlüsselwörter
- NF-κB
- TNF-α
- growth factor
- osteoarthritis
- platelet lysate
- Analgesics
- Animals
- Arthritis
- Blood Platelets
- Cell Survival
- Chondrocytes
- Gene Expression Regulation
- Humans
- Iodoacetic Acid
- Male
- NF-kappa B
- Osteoarthritis
- Pain
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Tumor Necrosis Factor-alpha
- Sprache
- eng
- Country
- United States
- Paginierung
- 2797 - 2811
- PII
- 101952
- Datum der Veröffentlichung
- 2019
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Titel
- Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 11
Datenquelle: PubMed
- Beziehungen:
- Eigentum von