Efficient Production of Isoflavonoids by Astragalus membranaceus Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Jiao Jiao
- Qing-Yan Gai
- Yu-Jie Fu
- Wei Ma
- Xiao Peng
- Sheng-Nan Tan
- Thomas Efferth
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000347437500022&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1021/jf503839m
- eISSN
- 1520-5118
- Externe Identifier
- Clarivate Analytics Document Solution ID: AY2RS
- PubMed Identifier: 25483292
- ISSN
- 0021-8561
- Ausgabe der Veröffentlichung
- 52
- Zeitschrift
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Schlüsselwörter
- isoflavonoids
- hairy roots
- Astragalus membranaceus
- response surface methodology
- LC-MS/MS
- antioxidant activities
- Paginierung
- 12649 - 12658
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Titel
- Efficient Production of Isoflavonoids by <i>Astragalus membranaceus</i> Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts
- Sub types
- Article
- Ausgabe der Zeitschrift
- 62
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Autoren
- Jiao Jiao
- Qing-Yan Gai
- Yu-Jie Fu
- Wei Ma
- Xiao Peng
- Sheng-Nan Tan
- Thomas Efferth
- DOI
- 10.1021/jf503839m
- eISSN
- 1520-5118
- ISSN
- 0021-8561
- Ausgabe der Veröffentlichung
- 52
- Zeitschrift
- Journal of Agricultural and Food Chemistry
- Sprache
- en
- Online publication date
- 2014
- Paginierung
- 12649 - 12658
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Herausgeber
- American Chemical Society (ACS)
- Herausgeber URL
- http://dx.doi.org/10.1021/jf503839m
- Datum der Datenerfassung
- 2023
- Titel
- Efficient Production of Isoflavonoids by <i>Astragalus membranaceus</i> Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts
- Ausgabe der Zeitschrift
- 62
Datenquelle: Crossref
- Abstract
- In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.
- Addresses
- State Key Laboratory of Tree Genetics and Breeding and §Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University , Harbin, Heilongjiang 150040, People's Republic of China.
- Autoren
- Jiao Jiao
- Qing-Yan Gai
- Yu-Jie Fu
- Wei Ma
- Xiao Peng
- Sheng-Nan Tan
- Thomas Efferth
- Thomas Efferth
- DOI
- 10.1021/jf503839m
- eISSN
- 1520-5118
- Externe Identifier
- PubMed Identifier: 25483292
- Funding acknowledgements
- National Natural Science Foundation of China: 31270618
- National Natural Science Foundation of China: 81274010
- Outstanding Youth Fund of Heilongjiang Province: JC201101
- Ministry of Science and Technology of the People's Republic of China: 2012GB23600641
- Ministry of Education of the People's Republic of China: 2572014AA06
- Open access
- false
- ISSN
- 0021-8561
- Ausgabe der Veröffentlichung
- 52
- Zeitschrift
- Journal of agricultural and food chemistry
- Schlüsselwörter
- Plant Roots
- Isoflavones
- Drugs, Chinese Herbal
- Antioxidants
- Cell Culture Techniques
- Astragalus propinquus
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2014
- Paginierung
- 12649 - 12658
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum der Datenerfassung
- 2014
- Titel
- Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 62
Datenquelle: Europe PubMed Central
- Abstract
- In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.
- Autoren
- Jiao Jiao
- Qing-Yan Gai
- Yu-Jie Fu
- Wei Ma
- Xiao Peng
- Sheng-Nan Tan
- Thomas Efferth
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/25483292
- DOI
- 10.1021/jf503839m
- eISSN
- 1520-5118
- Ausgabe der Veröffentlichung
- 52
- Zeitschrift
- J Agric Food Chem
- Schlüsselwörter
- Astragalus membranaceus
- LC−MS/MS
- antioxidant activities
- hairy roots
- isoflavonoids
- response surface methodology
- Antioxidants
- Astragalus propinquus
- Cell Culture Techniques
- Drugs, Chinese Herbal
- Isoflavones
- Plant Roots
- Sprache
- eng
- Country
- United States
- Paginierung
- 12649 - 12658
- Datum der Veröffentlichung
- 2014
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2015
- Titel
- Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 62
Datenquelle: PubMed
- Beziehungen:
- Eigentum von