Efficient Production of Isoflavonoids by Astragalus membranaceus Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts

Autoren

Jiao Jiao
Qing-Yan Gai
Yu-Jie Fu
Wei Ma
Xiao Peng
Sheng-Nan Tan
Thomas Efferth

DOI

10.1021/jf503839m

eISSN

1520-5118

ISSN

0021-8561

Ausgabe der Veröffentlichung

52

Zeitschrift

Journal of Agricultural and Food Chemistry

Sprache

en

Online publication date

2014

Paginierung

12649 - 12658

Datum der Veröffentlichung

2014

Status

Published

Herausgeber

American Chemical Society (ACS)

Herausgeber URL

http://dx.doi.org/10.1021/jf503839m

Datum der Datenerfassung

2023

Titel

Efficient Production of Isoflavonoids by <i>Astragalus membranaceus</i> Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts

Ausgabe der Zeitschrift

62

Data source: Crossref

Abstract

In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.

Addresses

State Key Laboratory of Tree Genetics and Breeding and §Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University , Harbin, Heilongjiang 150040, People's Republic of China.

Autoren

Jiao Jiao
Qing-Yan Gai
Yu-Jie Fu
Wei Ma
Xiao Peng
Sheng-Nan Tan
Thomas Efferth
Thomas Efferth

DOI

10.1021/jf503839m

eISSN

1520-5118

Externe Identifier

PubMed Identifier: 25483292

Funding acknowledgements

National Natural Science Foundation of China: 31270618
National Natural Science Foundation of China: 81274010
Outstanding Youth Fund of Heilongjiang Province: JC201101
Ministry of Science and Technology of the People's Republic of China: 2012GB23600641
Ministry of Education of the People's Republic of China: 2572014AA06

Open access

false

ISSN

0021-8561

Ausgabe der Veröffentlichung

52

Zeitschrift

Journal of agricultural and food chemistry

Schlüsselwörter

Plant Roots
Isoflavones
Drugs, Chinese Herbal
Antioxidants
Cell Culture Techniques
Astragalus propinquus

Sprache

eng

Medium

Print-Electronic

Online publication date

2014

Paginierung

12649 - 12658

Datum der Veröffentlichung

2014

Status

Published

Datum der Datenerfassung

2014

Titel

Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts.

Sub types

Research Support, Non-U.S. Gov't
Journal Article

Ausgabe der Zeitschrift

62

Data source: Europe PubMed Central

Abstract

In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.

Autoren

Jiao Jiao
Qing-Yan Gai
Yu-Jie Fu
Wei Ma
Xiao Peng
Sheng-Nan Tan
Thomas Efferth

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/25483292

DOI

10.1021/jf503839m

eISSN

1520-5118

Ausgabe der Veröffentlichung

52

Zeitschrift

J Agric Food Chem

Schlüsselwörter

Astragalus membranaceus
LC−MS/MS
antioxidant activities
hairy roots
isoflavonoids
response surface methodology
Antioxidants
Astragalus propinquus
Cell Culture Techniques
Drugs, Chinese Herbal
Isoflavones
Plant Roots

Sprache

eng

Country

United States

Paginierung

12649 - 12658

Datum der Veröffentlichung

2014

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2015

Titel

Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts.

Sub types

Journal Article
Research Support, Non-U.S. Gov't

Ausgabe der Zeitschrift

62

Data source: PubMed

Efficient Production of Isoflavonoids by Astragalus membranaceus Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts

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