CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Jens Schittenhelm
- Perikles Simon
- Patrick N Harter
- Cornelia Zachskorn
- Holger Schlaszus
- Frauke Röttger
- Monika Winkels
- Michael Weller
- Richard Meyermann
- Michel Mittelbronn
- Sammlungen
- metadata
- ISSN
- 1365-2559
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Histopathology
- Schlüsselwörter
- 796 Sport
- 796 Athletic and outdoor sports and games
- Sprache
- eng
- Paginierung
- Seiten: 739 - 749
- Datum der Veröffentlichung
- 2011
- Herausgeber URL
- http://dx.doi.org/10.1111/j.1365-2559.2011.03801.x
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms
- Ausgabe der Zeitschrift
- 58
Data source: METADATA.UB
- Other metadata sources:
-
- Autoren
- Jens Schittenhelm
- Perikles Simon
- Patrick N Harter
- Cornelia Zachskorn
- Holger Schlaszus
- Frauke Roettger
- Monika Winkels
- Michael Weller
- Richard Meyermann
- Michel Mittelbronn
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000289465400010&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1111/j.1365-2559.2011.03801.x
- Externe Identifier
- Clarivate Analytics Document Solution ID: 749KQ
- PubMed Identifier: 21438906
- ISSN
- 0309-0167
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- HISTOPATHOLOGY
- Schlüsselwörter
- CD133
- CNS neoplasms
- stem cells
- Paginierung
- 739 - 749
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Titel
- CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms
- Sub types
- Article
- Ausgabe der Zeitschrift
- 58
Data source: Web of Science (Lite)
- Autoren
- Jens Schittenhelm
- Perikles Simon
- Patrick N Harter
- Cornelia Zachskorn
- Holger Schlaszus
- Frauke Röttger
- Monika Winkels
- Michael Weller
- Richard Meyermann
- Michel Mittelbronn
- DOI
- 10.1111/j.1365-2559.2011.03801.x
- ISSN
- 0309-0167
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Histopathology
- Sprache
- en
- Online publication date
- 2011
- Paginierung
- 739 - 749
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1111/j.1365-2559.2011.03801.x
- Datum der Datenerfassung
- 2021
- Titel
- CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms
- Ausgabe der Zeitschrift
- 58
Data source: Crossref
- Abstract
- <h4>Aims</h4>CD133 is considered to be a marker for brain tumour-initiating cells. However, most data on CD133 are derived from animal or in-vitro studies. The aim of this study was to characterize CD133 expression, and the distribution and morphological features of CD133(+) cells, in primary and secondary human central nervous system (CNS) neoplasms.<h4>Methods and results</h4>Tumours were analysed by real-time reverse transcription polymerase chain reaction, western blot, flow cytometry and immunohistochemistry. Our results show that only small round blue cell tumours (SRBCTs) exhibit strong and consistent CD133 expression. Interestingly, glioblastomas, large-cell carcinomas and sarcomas were negative for CD133. Only glioblastomas with a focal small-cell component exhibited CD133 immunoreactivity in the SRBCT component. In addition, CD133 expression did not correlate with the expression of other markers associated with stem cell differentiation, including CD15 and nestin.<h4>Conclusions</h4>CD133 expression in human CNS neoplasms is independent of the grade of malignancy but strongly correlates with SRBCT morphology. Together with recent findings showing that CD133 is quickly upregulated upon hypoxia and that CD133(-) cells can also exhibit stem cell properties, our data strongly question the suitability of CD133 as a brain tumour stem cell marker in vivo.
- Addresses
- Institute of Brain Research, University of Tuebingen, Tuebingen, Germany.
- Autoren
- Jens Schittenhelm
- Perikles Simon
- Patrick N Harter
- Cornelia Zachskorn
- Holger Schlaszus
- Frauke Röttger
- Monika Winkels
- Michael Weller
- Richard Meyermann
- Michel Mittelbronn
- DOI
- 10.1111/j.1365-2559.2011.03801.x
- eISSN
- 1365-2559
- Externe Identifier
- PubMed Identifier: 21438906
- Open access
- false
- ISSN
- 0309-0167
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Histopathology
- Schlüsselwörter
- Humans
- Central Nervous System Neoplasms
- Brain Neoplasms
- Intermediate Filament Proteins
- Glycoproteins
- Peptides
- Nerve Tissue Proteins
- Protein Isoforms
- Antigens, CD
- Flow Cytometry
- Immunohistochemistry
- Neoplastic Stem Cells
- Nestin
- AC133 Antigen
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2011
- Paginierung
- 739 - 749
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum der Datenerfassung
- 2011
- Titel
- CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 58
Data source: Europe PubMed Central
- Abstract
- AIMS: CD133 is considered to be a marker for brain tumour-initiating cells. However, most data on CD133 are derived from animal or in-vitro studies. The aim of this study was to characterize CD133 expression, and the distribution and morphological features of CD133(+) cells, in primary and secondary human central nervous system (CNS) neoplasms. METHODS AND RESULTS: Tumours were analysed by real-time reverse transcription polymerase chain reaction, western blot, flow cytometry and immunohistochemistry. Our results show that only small round blue cell tumours (SRBCTs) exhibit strong and consistent CD133 expression. Interestingly, glioblastomas, large-cell carcinomas and sarcomas were negative for CD133. Only glioblastomas with a focal small-cell component exhibited CD133 immunoreactivity in the SRBCT component. In addition, CD133 expression did not correlate with the expression of other markers associated with stem cell differentiation, including CD15 and nestin. CONCLUSIONS: CD133 expression in human CNS neoplasms is independent of the grade of malignancy but strongly correlates with SRBCT morphology. Together with recent findings showing that CD133 is quickly upregulated upon hypoxia and that CD133(-) cells can also exhibit stem cell properties, our data strongly question the suitability of CD133 as a brain tumour stem cell marker in vivo.
- Autoren
- Jens Schittenhelm
- Perikles Simon
- Patrick N Harter
- Cornelia Zachskorn
- Holger Schlaszus
- Frauke Röttger
- Monika Winkels
- Michael Weller
- Richard Meyermann
- Michel Mittelbronn
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/21438906
- DOI
- 10.1111/j.1365-2559.2011.03801.x
- eISSN
- 1365-2559
- Ausgabe der Veröffentlichung
- 5
- Zeitschrift
- Histopathology
- Schlüsselwörter
- AC133 Antigen
- Antigens, CD
- Brain Neoplasms
- Central Nervous System Neoplasms
- Flow Cytometry
- Glycoproteins
- Humans
- Immunohistochemistry
- Intermediate Filament Proteins
- Neoplastic Stem Cells
- Nerve Tissue Proteins
- Nestin
- Peptides
- Protein Isoforms
- Sprache
- eng
- Country
- England
- Paginierung
- 739 - 749
- Datum der Veröffentlichung
- 2011
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2011
- Titel
- CD133 expression is associated with small round blue cell tumour morphology in human central nervous system neoplasms.
- Sub types
- Journal Article
- Ausgabe der Zeitschrift
- 58
Data source: PubMed
- Beziehungen:
- Property of