Principle considerations for the use of transcriptomics in doping research

Autoren

Elmo WI Neuberger
Dirk A Moser
Perikles Simon

Autoren-URL

https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000296613200005&DestLinkType=FullRecord&DestApp=WOS_CPL

DOI

10.1002/dta.331

eISSN

1942-7611

Externe Identifier

Clarivate Analytics Document Solution ID: 842QE
PubMed Identifier: 22031504

ISSN

1942-7603

Ausgabe der Veröffentlichung

10

Zeitschrift

DRUG TESTING AND ANALYSIS

Schlüsselwörter

doping analysis
transcriptomics
RNA
peripheral blood

Paginierung

668 - 675

Datum der Veröffentlichung

2011

Status

Published

Titel

Principle considerations for the use of transcriptomics in doping research

Sub types

Review

Ausgabe der Zeitschrift

3

Data source: Web of Science (Lite)

Abstract

AbstractOver the course of the past decade, technical progress has enabled scientists to investigate genome‐wide RNA expression using microarray platforms. This transcriptomic approach represents a promising tool for the discovery of basic gene expression patterns and for identification of cellular signalling pathways under various conditions. Since doping substances have been shown to influence mRNA expression, it has been suggested that these changes can be detected by screening the blood transcriptome. In this review, we critically discuss the potential but also the pitfalls of this application as a tool in doping research. Transcriptomic approaches were considered to potentially provide researchers with a unique gene expression signature or with a specific biomarker for various physiological and pathophysiological conditions. Since transcriptomic approaches are considerably prone to biological and technical confounding factors that act on study subjects or samples, very strict guidelines for the use of transcriptomics in human study subjects have been developed. Typical field conditions associated with doping controls limit the feasibility of following these strict guidelines as there are too many variables counteracting a standardized procedure. After almost a decade of research using transcriptomic tools, it still remains a matter of future technological progress to identify the ultimate biomarker using technologies and/or methodologies that are sufficiently robust against typical biological and technical bias and that are valid in a court of law. Copyright © 2011 John Wiley & Sons, Ltd.

Autoren

Elmo WI Neuberger
Dirk A Moser
Perikles Simon

DOI

10.1002/dta.331

eISSN

1942-7611

ISSN

1942-7603

Ausgabe der Veröffentlichung

10

Zeitschrift

Drug Testing and Analysis

Sprache

en

Online publication date

2011

Paginierung

668 - 675

Datum der Veröffentlichung

2011

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1002/dta.331

Datum der Datenerfassung

2023

Titel

Principle considerations for the use of transcriptomics in doping research

Ausgabe der Zeitschrift

3

Data source: Crossref

Abstract

Over the course of the past decade, technical progress has enabled scientists to investigate genome-wide RNA expression using microarray platforms. This transcriptomic approach represents a promising tool for the discovery of basic gene expression patterns and for identification of cellular signalling pathways under various conditions. Since doping substances have been shown to influence mRNA expression, it has been suggested that these changes can be detected by screening the blood transcriptome. In this review, we critically discuss the potential but also the pitfalls of this application as a tool in doping research. Transcriptomic approaches were considered to potentially provide researchers with a unique gene expression signature or with a specific biomarker for various physiological and pathophysiological conditions. Since transcriptomic approaches are considerably prone to biological and technical confounding factors that act on study subjects or samples, very strict guidelines for the use of transcriptomics in human study subjects have been developed. Typical field conditions associated with doping controls limit the feasibility of following these strict guidelines as there are too many variables counteracting a standardized procedure. After almost a decade of research using transcriptomic tools, it still remains a matter of future technological progress to identify the ultimate biomarker using technologies and/or methodologies that are sufficiently robust against typical biological and technical bias and that are valid in a court of law.

Addresses

Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg-University, Mainz, Germany.

Autoren

Elmo WI Neuberger
Dirk A Moser
Perikles Simon

DOI

10.1002/dta.331

eISSN

1942-7611

Externe Identifier

PubMed Identifier: 22031504

Open access

false

ISSN

1942-7603

Ausgabe der Veröffentlichung

10

Zeitschrift

Drug testing and analysis

Schlüsselwörter

Blood Cells
Animals
Humans
RNA, Messenger
Gene Expression Profiling
Substance Abuse Detection
Gene Expression Regulation
Doping in Sports
Transcriptome

Sprache

eng

Medium

Print

Paginierung

668 - 675

Datum der Veröffentlichung

2011

Status

Published

Datum der Datenerfassung

2011

Titel

Principle considerations for the use of transcriptomics in doping research.

Sub types

Review
Journal Article

Ausgabe der Zeitschrift

3

Data source: Europe PubMed Central

Abstract

Over the course of the past decade, technical progress has enabled scientists to investigate genome-wide RNA expression using microarray platforms. This transcriptomic approach represents a promising tool for the discovery of basic gene expression patterns and for identification of cellular signalling pathways under various conditions. Since doping substances have been shown to influence mRNA expression, it has been suggested that these changes can be detected by screening the blood transcriptome. In this review, we critically discuss the potential but also the pitfalls of this application as a tool in doping research. Transcriptomic approaches were considered to potentially provide researchers with a unique gene expression signature or with a specific biomarker for various physiological and pathophysiological conditions. Since transcriptomic approaches are considerably prone to biological and technical confounding factors that act on study subjects or samples, very strict guidelines for the use of transcriptomics in human study subjects have been developed. Typical field conditions associated with doping controls limit the feasibility of following these strict guidelines as there are too many variables counteracting a standardized procedure. After almost a decade of research using transcriptomic tools, it still remains a matter of future technological progress to identify the ultimate biomarker using technologies and/or methodologies that are sufficiently robust against typical biological and technical bias and that are valid in a court of law.

Autoren

Elmo WI Neuberger
Dirk A Moser
Perikles Simon

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/22031504

DOI

10.1002/dta.331

eISSN

1942-7611

Ausgabe der Veröffentlichung

10

Zeitschrift

Drug Test Anal

Schlüsselwörter

Animals
Blood Cells
Doping in Sports
Gene Expression Profiling
Gene Expression Regulation
Humans
RNA, Messenger
Substance Abuse Detection
Transcriptome

Sprache

eng

Country

England

Paginierung

668 - 675

Datum der Veröffentlichung

2011

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2012

Titel

Principle considerations for the use of transcriptomics in doping research.

Sub types

Journal Article
Review

Ausgabe der Zeitschrift

3

Data source: PubMed

Principle considerations for the use of transcriptomics in doping research

Tools