Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Astrid Hasibeder
- Pamela Stein
- Ricardo Brandwijk
- Hansjörg Schild
- Markus Radsak
- Sammlungen
- metadata
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Paginierung
- Art. 15381
- Datum der Veröffentlichung
- 2015
- Herausgeber
- Nature Publ. Group
- Herausgeber URL
- http://dx.doi.org/10.1038/srep15381
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay
- Ausgabe der Zeitschrift
- 5
Data source: METADATA.UB
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Triggering receptor expressed on myeloid cells (TREM)-1 plays an important role in innate immune responses and is upregulated under infectious as well as non-infectious conditions. In addition, a soluble TREM-1 variant (sTREM-1) is detectable in sera or bronchoalveolar-lavage fluids from patients. Currently, various studies are difficult to compare, since the methods of detection by enzyme-linked immunosorbent assays (ELISA) vary among different research groups. In this study, we compared three different s-TREM-1 specific ELISAs and identified individual assay characteristics finding notable differences in sTREM-1 concentrations in part depending on the employed buffers. Investigating potential confounding factors for sTREM-1 detection, serum heat-inactivation (HI) showed improved recovery compared to non-HI (NHI) serum, reproducible by addition of complement and re-heat-inactivation. Hence we identified complement as a heat-sensitive confounder in some sTREM-1 ELISAs. We conclude that it is difficult to directly compare data of several studies, in particular if different ELISAs are engaged. Immunoassays for research use only are in general hampered by lack of standardization. Further standardization is needed until sTREM-1 ELISA is capable for better reproducibility of studies and clinical application.</jats:p>
- Autoren
- Astrid Hasibeder
- Pamela Stein
- Ricardo Brandwijk
- Hansjörg Schild
- Markus P Radsak
- DOI
- 10.1038/srep15381
- eISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific Reports
- Sprache
- en
- Artikelnummer
- 15381
- Online publication date
- 2015
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/srep15381
- Datum der Datenerfassung
- 2023
- Titel
- Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay
- Ausgabe der Zeitschrift
- 5
Data source: Crossref
- Author's licence
- CC-BY
- Autoren
- Astrid Hasibeder
- Pamela Stein
- Ricardo Brandwijk
- Hansjörg Schild
- Markus Radsak
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-OA-Publizieren (2012 - 2017)
- Resource version
- Published version
- DOI
- 10.1038/srep15381
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- Art. 15381
- Datum der Veröffentlichung
- 2015
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/7396
- Herausgeber
- Nature Publ. Group
- Herausgeber URL
- http://dx.doi.org/10.1038/srep15381
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay
- Ausgabe der Zeitschrift
- 5
Files
evaluation_and_validation_of_-20220710234653630.pdf
Data source: OPENSCIENCE.UB