Evaluating ancient Egyptian prescriptions today : anti-inflammatory activity of Ziziphus spina-christi

Publication type:
Journal article
Metadata:
Autoren
  • Onat Kadioglu
  • Stefan Jacob
  • Stefan Bohnert
  • Janine Nass
  • Mohamed EM Saeed
  • Hassan Khalid
  • Irmgard Merfort
  • Eckhard Thines
  • Tanja Pommerening
  • Thomas Efferth
Sammlungen
  • metadata
ISSN
1618-095X
Ausgabe der Veröffentlichung
3
Zeitschrift
Phytomedicine
Schlüsselwörter
  • 930 Alte Geschichte
  • 930 History of ancient world
Sprache
eng
Paginierung
Seiten: 293 - 306
Datum der Veröffentlichung
2016
Herausgeber
Elsevier
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2016.01.004
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Evaluating ancient Egyptian prescriptions today : anti-inflammatory activity of Ziziphus spina-christi
Ausgabe der Zeitschrift
23

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Onat Kadioglu
  • Stefan Jacob
  • Stefan Bohnert
  • Janine Nass
  • Mohamed EM Saeed
  • Hassan Khalid
  • Irmgard Merfort
  • Eckhard Thines
  • Tanja Pommerening
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000371781500007&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2016.01.004
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: DG0UP
  • PubMed Identifier: 26969383
ISSN
0944-7113
Ausgabe der Veröffentlichung
3
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Ancient Egyptian medicinal papyri
  • Ethnopharmacology
  • Inflammation
  • Microarray
  • Molecular docking
  • Rhamnaceae
Paginierung
293 - 306
Datum der Veröffentlichung
2016
Status
Published
Titel
Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of <i>Ziziphus spina-christi</i>
Sub types
  • Article
Ausgabe der Zeitschrift
23

Data source: Web of Science (Lite)

Autoren
  • Onat Kadioglu
  • Stefan Jacob
  • Stefan Bohnert
  • Janine Naß
  • Mohamed EM Saeed
  • Hassan Khalid
  • Irmgard Merfort
  • Eckhard Thines
  • Tanja Pommerening
  • Thomas Efferth
DOI
10.1016/j.phymed.2016.01.004
ISSN
0944-7113
Ausgabe der Veröffentlichung
3
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
293 - 306
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2016.01.004
Datum der Datenerfassung
2020
Titel
Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi
Ausgabe der Zeitschrift
23

Data source: Crossref

Abstract
<h4>Background</h4>Ziziphus spina-christi (L.) Desf. (Christ's Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects. Nowadays, Z. spina-christi, is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation. Pharmacological research undertaken to date suggests that it possesses anti-inflammatory, hypoglycemic, hypotensive and anti-microbial effects. The transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is critical in inflammation, proliferation and involved in various types of cancer. Identification of new anti-inflammatory compounds might be an effective strategy to target inflammatory disorders and cancer. Therefore, extracts from Z. spina-christi are investigated in terms of their anti-inflammatory effects. Our intention is to evaluate the effects of Z. spina-christi described in ancient Egyptian papyri, and to show whether the effects can be proven with modern pharmacological methods. Furthermore, we determine the active ingredients in crude extracts for their inhibitory activity toward NF-κB pathway.<h4>Materials and methods</h4>To determine the active ingredients of Z. spina-christi, we fractionated the extracts for bioassays and identified the active compounds. Epigallocatechin, gallocatechin, spinosin, 6''' feruloylspinosin and 6''' sinapoylspinosin and crude extracts of seed, leaf, root or stem were analyzed for their effect on NF-κB DNA binding by electromobility shift assay (EMSA) and nuclear translocation of NF-κB-p65 by Western blot analysis. The binding mode of the compounds to NF-κB pathway proteins was compared with the known inhibitor, MG-132, by in silico molecular docking calculations. Log10IC50 values of gallocatechin and epigallocatechin as two main compounds of the plant were correlated to the microarray-based mRNA expression of 79 inflammation-related genes in cell lines of the National Cancer Institute (NCI, USA) as determined. The expression of 17 genes significantly correlated to the log10IC50 values for gallocatechin or epigallocatechin.<h4>Results</h4>Nuclear p65 protein level decreased upon treatment with each extract and compound. Root and seed extracts inhibited NF-κB-DNA binding as shown by EMSA. The compounds showed comparable binding energies and similar docking poses as MG-132 on the target proteins.<h4>Conclusion</h4>Z. spina-christi might possess anti-inflammatory activity as assumed by ancient Egyptian prescriptions. Five compounds contributed to this bioactivity, i.e. epigallocatechin, gallocatechin, spinosin, 6''' feruloylspinosin and 6''' sinapoylspinosin as shown in vitro and in silico.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128 Mainz, Germany.
Autoren
  • Onat Kadioglu
  • Stefan Jacob
  • Stefan Bohnert
  • Janine Naß
  • Mohamed EM Saeed
  • Hassan Khalid
  • Irmgard Merfort
  • Eckhard Thines
  • Tanja Pommerening
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2016.01.004
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 26969383
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
3
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • Cell Line, Tumor
  • Humans
  • Ziziphus
  • Seeds
  • Plant Stems
  • Plant Roots
  • Plants, Medicinal
  • Inflammation
  • Leupeptins
  • Anti-Inflammatory Agents
  • Plant Extracts
  • History, Ancient
  • Transcription Factor RelA
  • Herbal Medicine
  • Molecular Docking Simulation
  • Egypt, Ancient
Sprache
eng
Medium
Print-Electronic
Online publication date
2016
Paginierung
293 - 306
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
Sub types
  • Historical Article
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: Europe PubMed Central

Abstract
BACKGROUND: Ziziphus spina-christi (L.) Desf. (Christ's Thorn Jujube) is a wild tree today found in Jordan, Israel, Egypt, and some parts of Africa, which was already in use as a medicinal plant in Ancient Egypt. In ancient Egyptian prescriptions, it was used in remedies against swellings, pain, and heat, and thus should have anti-inflammatory effects. Nowadays, Z. spina-christi, is used in Egypt (by Bedouins, and Nubians), the Arabian Peninsula, Jordan, Iraq, and Morocco against a wide range of illnesses, most of them associated with inflammation. Pharmacological research undertaken to date suggests that it possesses anti-inflammatory, hypoglycemic, hypotensive and anti-microbial effects. The transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is critical in inflammation, proliferation and involved in various types of cancer. Identification of new anti-inflammatory compounds might be an effective strategy to target inflammatory disorders and cancer. Therefore, extracts from Z. spina-christi are investigated in terms of their anti-inflammatory effects. Our intention is to evaluate the effects of Z. spina-christi described in ancient Egyptian papyri, and to show whether the effects can be proven with modern pharmacological methods. Furthermore, we determine the active ingredients in crude extracts for their inhibitory activity toward NF-κB pathway. MATERIALS AND METHODS: To determine the active ingredients of Z. spina-christi, we fractionated the extracts for bioassays and identified the active compounds. Epigallocatechin, gallocatechin, spinosin, 6''' feruloylspinosin and 6''' sinapoylspinosin and crude extracts of seed, leaf, root or stem were analyzed for their effect on NF-κB DNA binding by electromobility shift assay (EMSA) and nuclear translocation of NF-κB-p65 by Western blot analysis. The binding mode of the compounds to NF-κB pathway proteins was compared with the known inhibitor, MG-132, by in silico molecular docking calculations. Log10IC50 values of gallocatechin and epigallocatechin as two main compounds of the plant were correlated to the microarray-based mRNA expression of 79 inflammation-related genes in cell lines of the National Cancer Institute (NCI, USA) as determined. The expression of 17 genes significantly correlated to the log10IC50 values for gallocatechin or epigallocatechin. RESULTS: Nuclear p65 protein level decreased upon treatment with each extract and compound. Root and seed extracts inhibited NF-κB-DNA binding as shown by EMSA. The compounds showed comparable binding energies and similar docking poses as MG-132 on the target proteins. CONCLUSION: Z. spina-christi might possess anti-inflammatory activity as assumed by ancient Egyptian prescriptions. Five compounds contributed to this bioactivity, i.e. epigallocatechin, gallocatechin, spinosin, 6''' feruloylspinosin and 6''' sinapoylspinosin as shown in vitro and in silico.
Date of acceptance
2016
Autoren
  • Onat Kadioglu
  • Stefan Jacob
  • Stefan Bohnert
  • Janine Naß
  • Mohamed EM Saeed
  • Hassan Khalid
  • Irmgard Merfort
  • Eckhard Thines
  • Tanja Pommerening
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26969383
DOI
10.1016/j.phymed.2016.01.004
eISSN
1618-095X
Ausgabe der Veröffentlichung
3
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Ancient Egyptian medicinal papyri
  • Ethnopharmacology
  • Inflammation
  • Microarray
  • Molecular docking
  • Rhamnaceae
  • Anti-Inflammatory Agents
  • Cell Line, Tumor
  • Egypt, Ancient
  • Herbal Medicine
  • History, Ancient
  • Humans
  • Inflammation
  • Leupeptins
  • Molecular Docking Simulation
  • Plant Extracts
  • Plant Roots
  • Plant Stems
  • Plants, Medicinal
  • Seeds
  • Transcription Factor RelA
  • Ziziphus
Sprache
eng
Country
Germany
Paginierung
293 - 306
PII
S0944-7113(16)00022-2
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Evaluating ancient Egyptian prescriptions today: Anti-inflammatory activity of Ziziphus spina-christi.
Sub types
  • Historical Article
  • Journal Article
Ausgabe der Zeitschrift
23

Data source: PubMed

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