Cytotoxicity and modes of action of four naturally occuring Benzophenones : 2,2',5,6'-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol

Publication type:
Journal article
Metadata:
Autoren
  • Victor Kuete
  • Patricia D Tchakam
  • Benjamin Wiench
  • Bathelemy Ngameni
  • Hippolyte K Wabo
  • Michel F Tala
  • Marlyse L Moungang
  • Bonaventure T Ngadjui
  • Tetsuya Murayama
  • Thomas Efferth
Sammlungen
  • metadata
ISSN
1618-095X
Ausgabe der Veröffentlichung
6
Zeitschrift
Phytomedicine
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Paginierung
Seiten: 528 - 536
Datum der Veröffentlichung
2013
Herausgeber
Elsevier
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2013.02.003
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Cytotoxicity and modes of action of four naturally occuring Benzophenones : 2,2',5,6'-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol
Ausgabe der Zeitschrift
20

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Victor Kuete
  • Patricia D Tchakam
  • Benjamin Wiench
  • Bathelemy Ngameni
  • Hippolyte K Wabo
  • Michel F Tala
  • Marlyse L Moungang
  • Bonaventure T Ngadjui
  • Tetsuya Murayama
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000319181900009&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1016/j.phymed.2013.02.003
eISSN
1618-095X
Externe Identifier
  • Clarivate Analytics Document Solution ID: 147QK
  • PubMed Identifier: 23507522
ISSN
0944-7113
Ausgabe der Veröffentlichung
6
Zeitschrift
PHYTOMEDICINE
Schlüsselwörter
  • Benzophenones
  • Plants
  • Cytotoxicity
  • Cell cycle distribution
  • Mitochondrial membrane potential
Paginierung
528 - 536
Datum der Veröffentlichung
2013
Status
Published
Titel
Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2′,5,6′-Tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol
Sub types
  • Article
Ausgabe der Zeitschrift
20

Data source: Web of Science (Lite)

Autoren
  • Victor Kuete
  • Patricia D Tchakam
  • Benjamin Wiench
  • Bathelemy Ngameni
  • Hippolyte K Wabo
  • Michel F Tala
  • Marlyse L Moungang
  • Bonaventure T Ngadjui
  • Tetsuya Murayama
  • Thomas Efferth
DOI
10.1016/j.phymed.2013.02.003
ISSN
0944-7113
Ausgabe der Veröffentlichung
6
Zeitschrift
Phytomedicine
Sprache
en
Paginierung
528 - 536
Datum der Veröffentlichung
2013
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.phymed.2013.02.003
Datum der Datenerfassung
2018
Titel
Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2′,5,6′-Tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol
Ausgabe der Zeitschrift
20

Data source: Crossref

Abstract
<h4>Introduction</h4>The emergence of drug-resistant cancer cells drastically reduces the efficacy of many antineoplasic agents and, consequently, increases the frequency of therapeutic failure. Benzophenones are known to display many pharmacological properties including cytotoxic activities. The present study was aimed at investigating the cytotoxicity and the modes of action of four naturally occurring benzophenones 2,2',5,6'-tetrahydroxybenzophenone (1), isogarcinol (2), isoxanthochymol (3) and guttiferone E (4) on a panel of eleven cancer cell lines including various sensitive and drug-resistant phenotypes.<h4>Methods</h4>The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 2-4. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS).<h4>Results</h4>The four tested benzophenones inhibited the proliferation of all tested cancer cell lines including sensitive and drug-resistant phenotypes. Collateral sensitivity of cancer cells to compounds 1-4 was generally better than to doxorubicin. Compound 2 showed the best activity with IC50 values below or around 1 μM against HCT116 colon carcinoma cells (p53+/+) (0.86 μM) and leukemia CCRF-CEM (1.38 μM) cell lines. Compounds 2-4 strongly induced apoptosis in CCRF-CEM cells via caspases 3/7, caspase 8 and caspase 9 activation and disruption of MMP.<h4>Conclusions</h4>The studied benzophenones are cytotoxic compounds that deserve more detailed exploration in the future, to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany.
Autoren
  • Victor Kuete
  • Patricia D Tchakam
  • Benjamin Wiench
  • Bathelemy Ngameni
  • Hippolyte K Wabo
  • Michel F Tala
  • Marlyse L Moungang
  • Bonaventure T Ngadjui
  • Tetsuya Murayama
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1016/j.phymed.2013.02.003
eISSN
1618-095X
Externe Identifier
  • PubMed Identifier: 23507522
Open access
false
ISSN
0944-7113
Ausgabe der Veröffentlichung
6
Zeitschrift
Phytomedicine : international journal of phytotherapy and phytopharmacology
Schlüsselwörter
  • HCT116 Cells
  • HL-60 Cells
  • Humans
  • Neoplasms
  • Leukemia
  • Carcinoma
  • Colonic Neoplasms
  • Reactive Oxygen Species
  • Benzophenones
  • Doxorubicin
  • Caspases
  • Matrix Metalloproteinases
  • Plant Extracts
  • Antineoplastic Agents, Phytogenic
  • Phytotherapy
  • Inhibitory Concentration 50
  • Apoptosis
  • Cell Proliferation
  • Drug Resistance, Neoplasm
  • Phenotype
Sprache
eng
Medium
Print-Electronic
Online publication date
2013
Paginierung
528 - 536
Datum der Veröffentlichung
2013
Status
Published
Datum der Datenerfassung
2013
Titel
Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2',5,6'-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
20

Data source: Europe PubMed Central

Abstract
INTRODUCTION: The emergence of drug-resistant cancer cells drastically reduces the efficacy of many antineoplasic agents and, consequently, increases the frequency of therapeutic failure. Benzophenones are known to display many pharmacological properties including cytotoxic activities. The present study was aimed at investigating the cytotoxicity and the modes of action of four naturally occurring benzophenones 2,2',5,6'-tetrahydroxybenzophenone (1), isogarcinol (2), isoxanthochymol (3) and guttiferone E (4) on a panel of eleven cancer cell lines including various sensitive and drug-resistant phenotypes. METHODS: The cytotoxicity of the compounds was determined using a resazurin reduction assay, whereas the caspase-Glo assay was used to detect the activation of caspases 3/7, caspase 8 and caspase 9 in cells treated with compounds 2-4. Flow cytometry was used for cell cycle analysis and detection of apoptotic cells, analysis of mitochondrial membrane potential (MMP) as well as measurement of reactive oxygen species (ROS). RESULTS: The four tested benzophenones inhibited the proliferation of all tested cancer cell lines including sensitive and drug-resistant phenotypes. Collateral sensitivity of cancer cells to compounds 1-4 was generally better than to doxorubicin. Compound 2 showed the best activity with IC50 values below or around 1 μM against HCT116 colon carcinoma cells (p53+/+) (0.86 μM) and leukemia CCRF-CEM (1.38 μM) cell lines. Compounds 2-4 strongly induced apoptosis in CCRF-CEM cells via caspases 3/7, caspase 8 and caspase 9 activation and disruption of MMP. CONCLUSIONS: The studied benzophenones are cytotoxic compounds that deserve more detailed exploration in the future, to develop novel anticancer drugs against sensitive and otherwise drug-resistant phenotypes.
Date of acceptance
2013
Autoren
  • Victor Kuete
  • Patricia D Tchakam
  • Benjamin Wiench
  • Bathelemy Ngameni
  • Hippolyte K Wabo
  • Michel F Tala
  • Marlyse L Moungang
  • Bonaventure T Ngadjui
  • Tetsuya Murayama
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/23507522
DOI
10.1016/j.phymed.2013.02.003
eISSN
1618-095X
Ausgabe der Veröffentlichung
6
Zeitschrift
Phytomedicine
Schlüsselwörter
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Benzophenones
  • Carcinoma
  • Caspases
  • Cell Proliferation
  • Colonic Neoplasms
  • Doxorubicin
  • Drug Resistance, Neoplasm
  • HCT116 Cells
  • HL-60 Cells
  • Humans
  • Inhibitory Concentration 50
  • Leukemia
  • Matrix Metalloproteinases
  • Neoplasms
  • Phenotype
  • Phytotherapy
  • Plant Extracts
  • Reactive Oxygen Species
Sprache
eng
Country
Germany
Paginierung
528 - 536
PII
S0944-7113(13)00049-4
Datum der Veröffentlichung
2013
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2',5,6'-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
20

Data source: PubMed

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