Sfp-type 4 '-phosphopantetheinyl transferase is indispensable for fungal pathogenicity
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Ralf Horbach
- Alexander Graf
- Fabian Weihmann
- Luis Antelo
- Sebastian Mathea
- Johannes Liermann
- Till Opatz
- Eckhard Thines
- Jesus Aguirre
- Holger B Deising
- Sammlungen
- metadata
- ISSN
- 1040-4651
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- The plant cell
- Schlüsselwörter
- 540 Chemie
- 540 Chemistry and allied sciences
- Sprache
- eng
- Paginierung
- Seiten: 3379 - 3396
- Datum der Veröffentlichung
- 2009
- Herausgeber
- Soc.
- Herausgeber URL
- http://dx.doi.org/10.1105/tpc.108.064188
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Sfp-type 4 '-phosphopantetheinyl transferase is indispensable for fungal pathogenicity
- Ausgabe der Zeitschrift
- 21
Data source: METADATA.UB
- Other metadata sources:
-
- Autoren
- Ralf Horbach
- Alexander Graf
- Fabian Weihmann
- Luis Antelo
- Sebastian Mathea
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Jesus Aguirre
- Holger B Deising
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000272252100030&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1105/tpc.108.064188
- eISSN
- 1532-298X
- Externe Identifier
- Clarivate Analytics Document Solution ID: 525XW
- PubMed Identifier: 19880801
- ISSN
- 1040-4651
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- PLANT CELL
- Paginierung
- 3379 - 3396
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Titel
- Sfp-Type 4′-Phosphopantetheinyl Transferase Is Indispensable for Fungal Pathogenicity
- Sub types
- Article
- Ausgabe der Zeitschrift
- 21
Data source: Web of Science (Lite)
- Abstract
- <jats:title>Abstract</jats:title><jats:p>In filamentous fungi, Sfp-type 4′-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary (α-aminoadipate reductase [AAR]) and secondary (polyketide synthases and nonribosomal peptide synthetases) metabolism. We cloned the PPTase gene PPT1 of the maize anthracnose fungus Colletotrichum graminicola and generated PPTase-deficient mutants (Δppt1). Δppt1 strains were auxotrophic for Lys, unable to synthesize siderophores, hypersensitive to reactive oxygen species, and unable to synthesize polyketides (PKs). A differential analysis of secondary metabolites produced by wild-type and Δppt1 strains led to the identification of six novel PKs. Infection-related morphogenesis was affected in Δppt1 strains. Rarely formed appressoria of Δppt1 strains were nonmelanized and ruptured on intact plant. The hyphae of Δppt1 strains colonized wounded maize (Zea mays) leaves but failed to generate necrotic anthracnose disease symptoms and were defective in asexual sporulation. To analyze the pleiotropic pathogenicity phenotype, we generated AAR-deficient mutants (Δaar1) and employed a melanin-deficient mutant (M1.502). Results indicated that PPT1 activates enzymes required at defined stages of infection. Melanization is required for cell wall rigidity and appressorium function, and Lys supplied by the AAR1 pathway is essential for necrotrophic development. As PPTase-deficient mutants of Magnaporthe oryzea were also nonpathogenic, we conclude that PPTases represent a novel fungal pathogenicity factor.</jats:p>
- Autoren
- Ralf Horbach
- Alexander Graf
- Fabian Weihmann
- Luis Antelo
- Sebastian Mathea
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Jesús Aguirre
- Holger B Deising
- DOI
- 10.1105/tpc.108.064188
- eISSN
- 1532-298X
- ISSN
- 1040-4651
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- The Plant Cell
- Sprache
- en
- Online publication date
- 2009
- Paginierung
- 3379 - 3396
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Oxford University Press (OUP)
- Herausgeber URL
- http://dx.doi.org/10.1105/tpc.108.064188
- Datum der Datenerfassung
- 2024
- Titel
- Sfp-Type 4′-Phosphopantetheinyl Transferase Is Indispensable for Fungal Pathogenicity
- Ausgabe der Zeitschrift
- 21
Data source: Crossref
- Abstract
- In filamentous fungi, Sfp-type 4'-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary (alpha-aminoadipate reductase [AAR]) and secondary (polyketide synthases and nonribosomal peptide synthetases) metabolism. We cloned the PPTase gene PPT1 of the maize anthracnose fungus Colletotrichum graminicola and generated PPTase-deficient mutants (Deltappt1). Deltappt1 strains were auxotrophic for Lys, unable to synthesize siderophores, hypersensitive to reactive oxygen species, and unable to synthesize polyketides (PKs). A differential analysis of secondary metabolites produced by wild-type and Deltappt1 strains led to the identification of six novel PKs. Infection-related morphogenesis was affected in Deltappt1 strains. Rarely formed appressoria of Deltappt1 strains were nonmelanized and ruptured on intact plant. The hyphae of Deltappt1 strains colonized wounded maize (Zea mays) leaves but failed to generate necrotic anthracnose disease symptoms and were defective in asexual sporulation. To analyze the pleiotropic pathogenicity phenotype, we generated AAR-deficient mutants (Deltaaar1) and employed a melanin-deficient mutant (M1.502). Results indicated that PPT1 activates enzymes required at defined stages of infection. Melanization is required for cell wall rigidity and appressorium function, and Lys supplied by the AAR1 pathway is essential for necrotrophic development. As PPTase-deficient mutants of Magnaporthe oryzea were also nonpathogenic, we conclude that PPTases represent a novel fungal pathogenicity factor.
- Addresses
- Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät III, Institut für Agrar und Ernährungswissenschaften, Phytopathologie und Pflanzenschutz, D-06099 Halle (Saale), Germany.
- Autoren
- Ralf Horbach
- Alexander Graf
- Fabian Weihmann
- Luis Antelo
- Sebastian Mathea
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Jesús Aguirre
- Holger B Deising
- DOI
- 10.1105/tpc.108.064188
- eISSN
- 1532-298X
- Externe Identifier
- PubMed Identifier: 19880801
- Open access
- false
- ISSN
- 1040-4651
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- The Plant cell
- Schlüsselwörter
- Magnaporthe
- Colletotrichum
- Transferases (Other Substituted Phosphate Groups)
- Bacterial Proteins
- Fungal Proteins
- Microscopy, Fluorescence
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Polymerase Chain Reaction
- Virulence
- Models, Biological
- Molecular Sequence Data
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2009
- Paginierung
- 3379 - 3396
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2009
- Titel
- Sfp-type 4'-phosphopantetheinyl transferase is indispensable for fungal pathogenicity.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 21
Data source: Europe PubMed Central
- Abstract
- In filamentous fungi, Sfp-type 4'-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary (alpha-aminoadipate reductase [AAR]) and secondary (polyketide synthases and nonribosomal peptide synthetases) metabolism. We cloned the PPTase gene PPT1 of the maize anthracnose fungus Colletotrichum graminicola and generated PPTase-deficient mutants (Deltappt1). Deltappt1 strains were auxotrophic for Lys, unable to synthesize siderophores, hypersensitive to reactive oxygen species, and unable to synthesize polyketides (PKs). A differential analysis of secondary metabolites produced by wild-type and Deltappt1 strains led to the identification of six novel PKs. Infection-related morphogenesis was affected in Deltappt1 strains. Rarely formed appressoria of Deltappt1 strains were nonmelanized and ruptured on intact plant. The hyphae of Deltappt1 strains colonized wounded maize (Zea mays) leaves but failed to generate necrotic anthracnose disease symptoms and were defective in asexual sporulation. To analyze the pleiotropic pathogenicity phenotype, we generated AAR-deficient mutants (Deltaaar1) and employed a melanin-deficient mutant (M1.502). Results indicated that PPT1 activates enzymes required at defined stages of infection. Melanization is required for cell wall rigidity and appressorium function, and Lys supplied by the AAR1 pathway is essential for necrotrophic development. As PPTase-deficient mutants of Magnaporthe oryzea were also nonpathogenic, we conclude that PPTases represent a novel fungal pathogenicity factor.
- Autoren
- Ralf Horbach
- Alexander Graf
- Fabian Weihmann
- Luis Antelo
- Sebastian Mathea
- Johannes C Liermann
- Till Opatz
- Eckhard Thines
- Jesús Aguirre
- Holger B Deising
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/19880801
- DOI
- 10.1105/tpc.108.064188
- eISSN
- 1532-298X
- Externe Identifier
- PubMed Central ID: PMC2782280
- Ausgabe der Veröffentlichung
- 10
- Zeitschrift
- Plant Cell
- Schlüsselwörter
- Bacterial Proteins
- Colletotrichum
- Fungal Proteins
- Magnaporthe
- Microscopy, Fluorescence
- Models, Biological
- Molecular Sequence Data
- Polymerase Chain Reaction
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Transferases (Other Substituted Phosphate Groups)
- Virulence
- Sprache
- eng
- Country
- England
- Paginierung
- 3379 - 3396
- PII
- tpc.108.064188
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Sfp-type 4'-phosphopantetheinyl transferase is indispensable for fungal pathogenicity.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 21
Data source: PubMed
- Beziehungen:
- Property of