Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells

Publication type:
Journal article
Metadata:
Autoren
  • Victor Kuete
  • Aimé G Fankam
  • Benjamin Wiench
  • Thomas Efferth
Sammlungen
  • metadata
ISSN
1741-4288
Zeitschrift
Evidence-based complementary and alternative medicine
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Paginierung
Art. 285903
Datum der Veröffentlichung
2013
Herausgeber
Hindawi
Herausgeber URL
http://dx.doi.org/10.1155/2013/285903
Datum der Datenerfassung
2020
Datum, an dem der Datensatz öffentlich gemacht wurde
2020
Zugang
Public
Titel
Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells
Ausgabe der Zeitschrift
2013

Data source: METADATA.UB

Other metadata sources:
Autoren
  • Victor Kuete
  • Aime G Fankam
  • Benjamin Wiench
  • Thomas Efferth
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000325292700001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1155/2013/285903
eISSN
1741-4288
Externe Identifier
  • Clarivate Analytics Document Solution ID: 229UJ
  • PubMed Identifier: 24174974
ISSN
1741-427X
Zeitschrift
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
Artikelnummer
ARTN 285903
Datum der Veröffentlichung
2013
Status
Published
Titel
Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells
Sub types
  • Article
Ausgabe der Zeitschrift
2013

Data source: Web of Science (Lite)

Abstract
<jats:p><jats:italic>Introduction</jats:italic>. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants,<jats:italic>Gladiolus quartinianus</jats:italic>,<jats:italic>Vepris soyauxii</jats:italic>, and<jats:italic>Anonidium mannii</jats:italic>.<jats:italic>Methods</jats:italic>. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS).<jats:italic>Results</jats:italic>. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from<jats:italic>G. quartinianus</jats:italic>(GQW; 25.69%),<jats:italic>Vepris soyauxii</jats:italic>leaves (VSL; 29.82%), and<jats:italic>Anonidium mannii</jats:italic>leaves (AML; 31.58%). The lowest IC<jats:sub>50</jats:sub>values below 30 <jats:italic>μ</jats:italic>g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC<jats:sub>50</jats:sub>values for each plant were 4.09 <jats:italic>μ</jats:italic>g/mL, and 9.14 <jats:italic>μ</jats:italic>g/mL (against U87MG.Δ<jats:italic>EGFR</jats:italic>cells), respectively, for VSL and AML and 10.57 <jats:italic>μ</jats:italic>g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS.<jats:italic>Conclusion</jats:italic>. The three active plants may be a source for the development of new anticancer drugs.</jats:p>
Autoren
  • Victor Kuete
  • Aimé G Fankam
  • Benjamin Wiench
  • Thomas Efferth
DOI
10.1155/2013/285903
eISSN
1741-4288
ISSN
1741-427X
Zeitschrift
Evidence-Based Complementary and Alternative Medicine
Sprache
en
Paginierung
1 - 10
Datum der Veröffentlichung
2013
Status
Published
Herausgeber
Hindawi Limited
Herausgeber URL
http://dx.doi.org/10.1155/2013/285903
Datum der Datenerfassung
2020
Titel
Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells
Ausgabe der Zeitschrift
2013

Data source: Crossref

Abstract
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.
Addresses
  • Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Autoren
  • Victor Kuete
  • Aimé G Fankam
  • Benjamin Wiench
  • Thomas Efferth
  • Thomas Efferth
DOI
10.1155/2013/285903
eISSN
1741-4288
Externe Identifier
  • PubMed Identifier: 24174974
  • PubMed Central ID: PMC3794640
Open access
true
ISSN
1741-427X
Zeitschrift
Evidence-based complementary and alternative medicine : eCAM
Sprache
eng
Medium
Print-Electronic
Online publication date
2013
Open access status
Open Access
Paginierung
285903
Datum der Veröffentlichung
2013
Status
Published
Publisher licence
CC BY
Datum der Datenerfassung
2013
Titel
Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
2013

Files

https://downloads.hindawi.com/journals/ecam/2013/285903.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24174974/pdf/?tool=EBI https://europepmc.org/articles/PMC3794640?pdf=render

Data source: Europe PubMed Central

Abstract
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.
Date of acceptance
2013
Autoren
  • Victor Kuete
  • Aimé G Fankam
  • Benjamin Wiench
  • Thomas Efferth
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/24174974
DOI
10.1155/2013/285903
Externe Identifier
  • PubMed Central ID: PMC3794640
ISSN
1741-427X
Zeitschrift
Evid Based Complement Alternat Med
Sprache
eng
Country
United States
Paginierung
285903
Datum der Veröffentlichung
2013
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2013
Titel
Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
2013

Data source: PubMed

Author's licence
CC-BY
Autoren
  • Victor Kuete
  • Aimé G Fankam
  • Benjamin Wiench
  • Thomas Efferth
Hosting institution
Universitätsbibliothek Mainz
Sammlungen
  • DFG-OA-Publizieren (2012 - 2017)
Resource version
Published version
DOI
10.1155/2013/285903
Funding acknowledgements
  • DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
File(s) embargoed
false
Open access
true
ISSN
1741-4288
Zeitschrift
Evidence-based complementary and alternative medicine
Schlüsselwörter
  • 570 Biowissenschaften
  • 570 Life sciences
Sprache
eng
Open access status
Open Access
Paginierung
Art. 285903
Datum der Veröffentlichung
2013
Public URL
https://openscience.ub.uni-mainz.de/handle/20.500.12030/7900
Herausgeber
Hindawi
Herausgeber URL
http://dx.doi.org/10.1155/2013/285903
Datum der Datenerfassung
2022
Datum, an dem der Datensatz öffentlich gemacht wurde
2022
Zugang
Public
Titel
Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells
Ausgabe der Zeitschrift
2013

Files

cytotoxicity_and_modes_of_act-20221006095214615.pdf

Data source: OPENSCIENCE.UB

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