Glaucoma related proteomic alterations in human retina samples
- Publication type:
- Journal article
- Metadata:
-
- Autoren
- Sebastian Funke
- Natarajan Perumal
- Sabine Beck
- Silke Gabel-Scheurich
- Carsten Schmelter
- Julia Teister
- Claudia Gerbig
- Oliver W Gramlich
- Norbert Pfeiffer
- Franz-Hermann Grus
- Sammlungen
- metadata
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Paginierung
- Art. 29759
- Datum der Veröffentlichung
- 2016
- Herausgeber
- Nature Publishing Group
- Herausgeber URL
- http://dx.doi.org/10.1038/srep29759
- Datum der Datenerfassung
- 2020
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2020
- Zugang
- Public
- Titel
- Glaucoma related proteomic alterations in human retina samples
- Ausgabe der Zeitschrift
- 6
Data source: METADATA.UB
- Other metadata sources:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma.</jats:p>
- Autoren
- Sebastian Funke
- Natarajan Perumal
- Sabine Beck
- Silke Gabel-Scheurich
- Carsten Schmelter
- Julia Teister
- Claudia Gerbig
- Oliver W Gramlich
- Norbert Pfeiffer
- Franz H Grus
- DOI
- 10.1038/srep29759
- eISSN
- 2045-2322
- Ausgabe der Veröffentlichung
- 1
- Zeitschrift
- Scientific Reports
- Sprache
- en
- Artikelnummer
- 29759
- Online publication date
- 2016
- Status
- Published online
- Herausgeber
- Springer Science and Business Media LLC
- Herausgeber URL
- http://dx.doi.org/10.1038/srep29759
- Datum der Datenerfassung
- 2023
- Titel
- Glaucoma related Proteomic Alterations in Human Retina Samples
- Ausgabe der Zeitschrift
- 6
Data source: Crossref
- Author's licence
- CC-BY
- Autoren
- Sebastian Funke
- Natarajan Perumal
- Sabine Beck
- Silke Gabel-Scheurich
- Carsten Schmelter
- Julia Teister
- Claudia Gerbig
- Oliver W Gramlich
- Norbert Pfeiffer
- Franz-Hermann Grus
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- DFG-OA-Publizieren (2012 - 2017)
- Resource version
- Published version
- DOI
- 10.1038/srep29759
- Funding acknowledgements
- DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 2045-2322
- Zeitschrift
- Scientific reports
- Schlüsselwörter
- 610 Medizin
- 610 Medical sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- Art. 29759
- Datum der Veröffentlichung
- 2016
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/7447
- Herausgeber
- Nature Publishing Group
- Herausgeber URL
- http://dx.doi.org/10.1038/srep29759
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- Glaucoma related proteomic alterations in human retina samples
- Ausgabe der Zeitschrift
- 6
Files
glaucoma_related_proteomic_al-20220714105734446.pdf
Data source: OPENSCIENCE.UB