Combined MRI-PET dissects dynamic changes in plant structures and functions

Abstract

SummaryUnravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non‐invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short‐lived radioactive carbon isotope 11C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species‐specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI–PET co‐registration opens the door for non‐invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.

Autoren

Siegfried Jahnke
Marion I Menzel
Dagmar Van Dusschoten
Gerhard W Roeb
Jonas Bühler
Senay Minwuyelet
Peter Blümler
Vicky M Temperton
Thomas Hombach
Matthias Streun
Simone Beer
Maryam Khodaverdi
Karl Ziemons
Heinz H Coenen
Ulrich Schurr

DOI

10.1111/j.1365-313x.2009.03888.x

eISSN

1365-313X

ISSN

0960-7412

Ausgabe der Veröffentlichung

4

Zeitschrift

The Plant Journal

Sprache

en

Online publication date

2009

Paginierung

634 - 644

Datum der Veröffentlichung

2009

Status

Published

Herausgeber

Wiley

Herausgeber URL

http://dx.doi.org/10.1111/j.1365-313x.2009.03888.x

Datum der Datenerfassung

2023

Titel

Combined MRI–PET dissects dynamic changes in plant structures and functions

Ausgabe der Zeitschrift

59

Data source: Crossref

Abstract

Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope (11)C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI-PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.

Addresses

ICG-3: Phytosphere, Forschungszentrum Jülich, 52425 Jülich, Germany. s.jahnke@fz-juelich.de

Autoren

Siegfried Jahnke
Marion I Menzel
Dagmar van Dusschoten
Gerhard W Roeb
Jonas Bühler
Senay Minwuyelet
Peter Blümler
Vicky M Temperton
Thomas Hombach
Matthias Streun
Simone Beer
Maryam Khodaverdi
Karl Ziemons
Heinz H Coenen
Ulrich Schurr

DOI

10.1111/j.1365-313x.2009.03888.x

eISSN

1365-313X

Externe Identifier

PubMed Identifier: 19392708

Open access

false

ISSN

0960-7412

Ausgabe der Veröffentlichung

4

Zeitschrift

The Plant journal : for cell and molecular biology

Schlüsselwörter

Raphanus
Beta vulgaris
Zea mays
Plant Shoots
Plant Roots
Carbon Radioisotopes
Positron-Emission Tomography
Magnetic Resonance Imaging

Sprache

eng

Medium

Print-Electronic

Online publication date

2009

Paginierung

634 - 644

Datum der Veröffentlichung

2009

Status

Published

Datum der Datenerfassung

2009

Titel

Combined MRI-PET dissects dynamic changes in plant structures and functions.

Sub types

Journal Article

Ausgabe der Zeitschrift

59

Data source: Europe PubMed Central

Abstract

Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope (11)C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI-PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.

Autoren

Siegfried Jahnke
Marion I Menzel
Dagmar van Dusschoten
Gerhard W Roeb
Jonas Bühler
Senay Minwuyelet
Peter Blümler
Vicky M Temperton
Thomas Hombach
Matthias Streun
Simone Beer
Maryam Khodaverdi
Karl Ziemons
Heinz H Coenen
Ulrich Schurr

Autoren-URL

https://www.ncbi.nlm.nih.gov/pubmed/19392708

DOI

10.1111/j.1365-313X.2009.03888.x

eISSN

1365-313X

Ausgabe der Veröffentlichung

4

Zeitschrift

Plant J

Schlüsselwörter

Beta vulgaris
Carbon Radioisotopes
Magnetic Resonance Imaging
Plant Roots
Plant Shoots
Positron-Emission Tomography
Raphanus
Zea mays

Sprache

eng

Country

England

Paginierung

634 - 644

PII

TPJ3888

Datum der Veröffentlichung

2009

Status

Published

Datum, an dem der Datensatz öffentlich gemacht wurde

2009

Titel

Combined MRI-PET dissects dynamic changes in plant structures and functions.

Sub types

Journal Article

Ausgabe der Zeitschrift

59

Data source: PubMed

Combined MRI-PET dissects dynamic changes in plant structures and functions

Tools