Fluidizing the Membrane by a Local Anesthetic: Phenylethanol Affects Membrane Protein Oligomerization

Publication type:
Journal article
Metadata:
Autoren
  • Veerappan Anbazhagan
  • Carmen Munz
  • Lydia Tome
  • Dirk Schneider
DOI
10.1016/j.jmb.2010.10.026
ISSN
0022-2836
Ausgabe der Veröffentlichung
5
Zeitschrift
Journal of Molecular Biology
Sprache
en
Paginierung
773 - 777
Datum der Veröffentlichung
2010
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.jmb.2010.10.026
Datum der Datenerfassung
2024
Titel
Fluidizing the Membrane by a Local Anesthetic: Phenylethanol Affects Membrane Protein Oligomerization
Ausgabe der Zeitschrift
404

Data source: Crossref

Other metadata sources:
Abstract
The exact mechanism of action of anesthetics is still an open question. While some observations suggest specific anesthetic-protein interactions, nonspecific perturbation of the lipid bilayer has also been suggested. Perturbations of bilayer properties could subsequently affect the structure and function of membrane proteins. Addition of the local anesthetic phenylethanol (PEtOH) to model membranes and intact Escherichia coli cells not only affected membrane fluidity but also severely altered the defined helix-helix interaction within the membrane. This experimental observation suggests that certain anesthetics modulate membrane physical properties and thereby indirectly affect transmembrane (TM) helix-helix interactions, which are not only involved in membrane protein folding and assembly but also important for TM signaling.
Addresses
  • Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz, Johann-Joachim-Becher-Weg 30, 55128 Mainz, Germany.
Autoren
  • Veerappan Anbazhagan
  • Carmen Munz
  • Lydia Tome
  • Dirk Schneider
DOI
10.1016/j.jmb.2010.10.026
eISSN
1089-8638
Externe Identifier
  • PubMed Identifier: 20970426
Open access
false
ISSN
0022-2836
Ausgabe der Veröffentlichung
5
Zeitschrift
Journal of molecular biology
Schlüsselwörter
  • Cell Membrane
  • Escherichia coli
  • Phenylethyl Alcohol
  • Escherichia coli Proteins
  • Membrane Proteins
  • Anesthetics, Local
  • Signal Transduction
  • Membrane Fluidity
  • Protein Structure, Secondary
  • Protein Binding
  • Models, Biological
  • Protein Interaction Domains and Motifs
  • Protein Multimerization
Sprache
eng
Medium
Print-Electronic
Online publication date
2010
Paginierung
773 - 777
Datum der Veröffentlichung
2010
Status
Published
Datum der Datenerfassung
2010
Titel
Fluidizing the membrane by a local anesthetic: phenylethanol affects membrane protein oligomerization.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
404

Data source: Europe PubMed Central

Abstract
The exact mechanism of action of anesthetics is still an open question. While some observations suggest specific anesthetic-protein interactions, nonspecific perturbation of the lipid bilayer has also been suggested. Perturbations of bilayer properties could subsequently affect the structure and function of membrane proteins. Addition of the local anesthetic phenylethanol (PEtOH) to model membranes and intact Escherichia coli cells not only affected membrane fluidity but also severely altered the defined helix-helix interaction within the membrane. This experimental observation suggests that certain anesthetics modulate membrane physical properties and thereby indirectly affect transmembrane (TM) helix-helix interactions, which are not only involved in membrane protein folding and assembly but also important for TM signaling.
Date of acceptance
2010
Autoren
  • Veerappan Anbazhagan
  • Carmen Munz
  • Lydia Tome
  • Dirk Schneider
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/20970426
DOI
10.1016/j.jmb.2010.10.026
eISSN
1089-8638
Ausgabe der Veröffentlichung
5
Zeitschrift
J Mol Biol
Schlüsselwörter
  • Anesthetics, Local
  • Cell Membrane
  • Escherichia coli
  • Escherichia coli Proteins
  • Membrane Fluidity
  • Membrane Proteins
  • Models, Biological
  • Phenylethyl Alcohol
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Protein Multimerization
  • Protein Structure, Secondary
  • Signal Transduction
Sprache
eng
Country
Netherlands
Paginierung
773 - 777
PII
S0022-2836(10)01134-4
Datum der Veröffentlichung
2010
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2010
Titel
Fluidizing the membrane by a local anesthetic: phenylethanol affects membrane protein oligomerization.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
404

Data source: PubMed

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