Specific and promiscuous functions of multiple DnaJ proteins in Synechocystis sp. PCC 6803

Publication type:
Journal article
Metadata:
Abstract
<jats:p>Cyanobacterial genomes typically encode multiple Hsp70 (DnaK) and Hsp40 (DnaJ) chaperones, and in the genome of the cyanobacterium<jats:italic>Synechocystis</jats:italic>PCC 6803, three DnaK proteins are encoded together with seven DnaJ proteins. While only two of the DnaJ proteins can complement the growth defect of an<jats:italic>Escherichia coli</jats:italic>Δ<jats:italic>dnaJ</jats:italic>strain, only disruption of the<jats:italic>dnaJ</jats:italic>gene<jats:italic>sll0897</jats:italic>resulted in a growth defect at elevated temperatures. Based on the domain structure and the phenotype observed following disruption of the encoding gene, Sll0897 can be classified as a canonical heat-shock protein in<jats:italic>Synechocystis</jats:italic>. Furthermore, most<jats:italic>dnaJ</jats:italic>genes could be deleted individually, whereas disruption of the gene encoding the DnaJ Sll1933 failed, which suggests an essential, yet undefined, function for Sll1933. Since after deletion of the remaining<jats:italic>dnaJ</jats:italic>genes the phenotypes were not altered, the functions of these DnaJs either are not critical or are taken over by the remaining DnaJs. Nevertheless, only the two<jats:italic>dnaJ</jats:italic>genes<jats:italic>sll0909</jats:italic>and<jats:italic>sll1384</jats:italic>could be disrupted in combination, suggesting physiological functions for the two encoded proteins which either are not overlapping and/or can be fulfilled by the remaining DnaJs in the double-disruption strain. Taken together, the present analysis indicates specific and promiscuous functions for multiple DnaJ proteins in<jats:italic>Synechocystis</jats:italic>.</jats:p>
Autoren
  • Eva Düppre
  • Eva Rupprecht
  • Dirk Schneider
DOI
10.1099/mic.0.045542-0
eISSN
1465-2080
ISSN
1350-0872
Ausgabe der Veröffentlichung
5
Zeitschrift
Microbiology
Sprache
en
Paginierung
1269 - 1278
Datum der Veröffentlichung
2011
Status
Published
Herausgeber
Microbiology Society
Herausgeber URL
http://dx.doi.org/10.1099/mic.0.045542-0
Datum der Datenerfassung
2021
Titel
Specific and promiscuous functions of multiple DnaJ proteins in Synechocystis sp. PCC 6803
Ausgabe der Zeitschrift
157

Data source: Crossref

Other metadata sources:
Abstract
Cyanobacterial genomes typically encode multiple Hsp70 (DnaK) and Hsp40 (DnaJ) chaperones, and in the genome of the cyanobacterium Synechocystis PCC 6803, three DnaK proteins are encoded together with seven DnaJ proteins. While only two of the DnaJ proteins can complement the growth defect of an Escherichia coli ΔdnaJ strain, only disruption of the dnaJ gene sll0897 resulted in a growth defect at elevated temperatures. Based on the domain structure and the phenotype observed following disruption of the encoding gene, Sll0897 can be classified as a canonical heat-shock protein in Synechocystis. Furthermore, most dnaJ genes could be deleted individually, whereas disruption of the gene encoding the DnaJ Sll1933 failed, which suggests an essential, yet undefined, function for Sll1933. Since after deletion of the remaining dnaJ genes the phenotypes were not altered, the functions of these DnaJs either are not critical or are taken over by the remaining DnaJs. Nevertheless, only the two dnaJ genes sll0909 and sll1384 could be disrupted in combination, suggesting physiological functions for the two encoded proteins which either are not overlapping and/or can be fulfilled by the remaining DnaJs in the double-disruption strain. Taken together, the present analysis indicates specific and promiscuous functions for multiple DnaJ proteins in Synechocystis.
Addresses
  • Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung (ZBMZ), Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Autoren
  • Eva Düppre
  • Eva Rupprecht
  • Dirk Schneider
DOI
10.1099/mic.0.045542-0
eISSN
1465-2080
Externe Identifier
  • PubMed Identifier: 21292744
Funding acknowledgements
  • Deutsche Forschungsgemeinschaft: FOR 929, SCHN 690/3-1
Open access
false
ISSN
1350-0872
Ausgabe der Veröffentlichung
Pt 5
Zeitschrift
Microbiology (Reading, England)
Schlüsselwörter
  • Synechocystis
  • Bacterial Proteins
  • Protein Structure, Tertiary
  • Multigene Family
  • HSP40 Heat-Shock Proteins
Sprache
eng
Medium
Print-Electronic
Online publication date
2011
Paginierung
1269 - 1278
Datum der Veröffentlichung
2011
Status
Published
Datum der Datenerfassung
2011
Titel
Specific and promiscuous functions of multiple DnaJ proteins in Synechocystis sp. PCC 6803.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
157

Data source: Europe PubMed Central

Abstract
Cyanobacterial genomes typically encode multiple Hsp70 (DnaK) and Hsp40 (DnaJ) chaperones, and in the genome of the cyanobacterium Synechocystis PCC 6803, three DnaK proteins are encoded together with seven DnaJ proteins. While only two of the DnaJ proteins can complement the growth defect of an Escherichia coli ΔdnaJ strain, only disruption of the dnaJ gene sll0897 resulted in a growth defect at elevated temperatures. Based on the domain structure and the phenotype observed following disruption of the encoding gene, Sll0897 can be classified as a canonical heat-shock protein in Synechocystis. Furthermore, most dnaJ genes could be deleted individually, whereas disruption of the gene encoding the DnaJ Sll1933 failed, which suggests an essential, yet undefined, function for Sll1933. Since after deletion of the remaining dnaJ genes the phenotypes were not altered, the functions of these DnaJs either are not critical or are taken over by the remaining DnaJs. Nevertheless, only the two dnaJ genes sll0909 and sll1384 could be disrupted in combination, suggesting physiological functions for the two encoded proteins which either are not overlapping and/or can be fulfilled by the remaining DnaJs in the double-disruption strain. Taken together, the present analysis indicates specific and promiscuous functions for multiple DnaJ proteins in Synechocystis.
Autoren
  • Eva Düppre
  • Eva Rupprecht
  • Dirk Schneider
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/21292744
DOI
10.1099/mic.0.045542-0
eISSN
1465-2080
Ausgabe der Veröffentlichung
Pt 5
Zeitschrift
Microbiology (Reading)
Schlüsselwörter
  • Bacterial Proteins
  • HSP40 Heat-Shock Proteins
  • Multigene Family
  • Protein Structure, Tertiary
  • Synechocystis
Sprache
eng
Country
England
Paginierung
1269 - 1278
Datum der Veröffentlichung
2011
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2011
Titel
Specific and promiscuous functions of multiple DnaJ proteins in Synechocystis sp. PCC 6803.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
157

Data source: PubMed

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