A Minimal Hydrophobicity Is Needed To Employ Amphiphilic p(HPMA)-co-p(LMA) Random Copolymers in Membrane Research

Publication type:
Journal article
Metadata:
Autoren
  • Michael Stangl
  • Mirjam Hemmelmann
  • Mareli Allmeroth
  • Rudolf Zentel
  • Dirk Schneider
DOI
10.1021/bi401611f
eISSN
1520-4995
ISSN
0006-2960
Ausgabe der Veröffentlichung
9
Zeitschrift
Biochemistry
Sprache
en
Online publication date
2014
Paginierung
1410 - 1419
Datum der Veröffentlichung
2014
Status
Published
Herausgeber
American Chemical Society (ACS)
Herausgeber URL
http://dx.doi.org/10.1021/bi401611f
Datum der Datenerfassung
2023
Titel
A Minimal Hydrophobicity Is Needed To Employ Amphiphilic p(HPMA)-co-p(LMA) Random Copolymers in Membrane Research
Ausgabe der Zeitschrift
53

Data source: Crossref

Other metadata sources:
Abstract
Because a polymer environment might be milder than a detergent micelle, amphiphilic polymers have attracted attention as alternatives to detergents in membrane biochemistry. The polymer poly[N-(2-hydroxypropyl)-methacrylamid] [p(HPMA)] has recently been modified with hydrophobic lauryl methacrylate (LMA) moieties, resulting in the synthesis of amphiphilic p(HPMA)-co-p(LMA) polymers. p(HPMA)-co-p(LMA) polymers with a LMA content of 5 or 15% have unstable hydrophobic cores. This, on one hand, promotes interactions of the hydrophobic LMA moieties with membranes, resulting in membrane rupture, but at the same time prevents formation of a hydrophobic, membrane mimetic environment that is sufficiently stable for the incorporation of transmembrane proteins. On the other hand, the p(HPMA)-co-p(LMA) polymer with a LMA content of 25% forms a stable hydrophobic core structure, which prevents hydrophobic interactions with membrane lipids but allows stable incorporation of membrane proteins. On the basis of our data, it becomes obvious that amphiphilic polymers have to have threshold hydrophobicities should an application in membrane protein research be anticipated.
Addresses
  • Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz , Johann-Joachim-Becher-Weg 30, 55128 Mainz, Germany.
Autoren
  • Michael Stangl
  • Mirjam Hemmelmann
  • Mareli Allmeroth
  • Rudolf Zentel
  • Dirk Schneider
DOI
10.1021/bi401611f
eISSN
1520-4995
Externe Identifier
  • PubMed Identifier: 24533898
Open access
false
ISSN
0006-2960
Ausgabe der Veröffentlichung
9
Zeitschrift
Biochemistry
Schlüsselwörter
  • Methacrylates
  • Polymers
  • Hydrophobic and Hydrophilic Interactions
Sprache
eng
Medium
Print-Electronic
Online publication date
2014
Paginierung
1410 - 1419
Datum der Veröffentlichung
2014
Status
Published
Datum der Datenerfassung
2014
Titel
A minimal hydrophobicity is needed to employ amphiphilic p(HPMA)-co-p(LMA) random copolymers in membrane research.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
53

Data source: Europe PubMed Central

Abstract
Because a polymer environment might be milder than a detergent micelle, amphiphilic polymers have attracted attention as alternatives to detergents in membrane biochemistry. The polymer poly[N-(2-hydroxypropyl)-methacrylamid] [p(HPMA)] has recently been modified with hydrophobic lauryl methacrylate (LMA) moieties, resulting in the synthesis of amphiphilic p(HPMA)-co-p(LMA) polymers. p(HPMA)-co-p(LMA) polymers with a LMA content of 5 or 15% have unstable hydrophobic cores. This, on one hand, promotes interactions of the hydrophobic LMA moieties with membranes, resulting in membrane rupture, but at the same time prevents formation of a hydrophobic, membrane mimetic environment that is sufficiently stable for the incorporation of transmembrane proteins. On the other hand, the p(HPMA)-co-p(LMA) polymer with a LMA content of 25% forms a stable hydrophobic core structure, which prevents hydrophobic interactions with membrane lipids but allows stable incorporation of membrane proteins. On the basis of our data, it becomes obvious that amphiphilic polymers have to have threshold hydrophobicities should an application in membrane protein research be anticipated.
Autoren
  • Michael Stangl
  • Mirjam Hemmelmann
  • Mareli Allmeroth
  • Rudolf Zentel
  • Dirk Schneider
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/24533898
DOI
10.1021/bi401611f
eISSN
1520-4995
Ausgabe der Veröffentlichung
9
Zeitschrift
Biochemistry
Schlüsselwörter
  • Hydrophobic and Hydrophilic Interactions
  • Methacrylates
  • Polymers
Sprache
eng
Country
United States
Paginierung
1410 - 1419
Datum der Veröffentlichung
2014
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2014
Titel
A minimal hydrophobicity is needed to employ amphiphilic p(HPMA)-co-p(LMA) random copolymers in membrane research.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
53

Data source: PubMed

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