Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols

Publication type:
Journal article
Metadata:
Autoren
  • Xiaoyan Zhang
  • Mihai Lomora
  • Tomaz Einfalt
  • Wolfgang Meier
  • Noreen Klein
  • Dirk Schneider
  • Cornelia G Palivan
DOI
10.1016/j.biomaterials.2016.02.042
ISSN
0142-9612
Zeitschrift
Biomaterials
Sprache
en
Paginierung
79 - 88
Datum der Veröffentlichung
2016
Status
Published
Herausgeber
Elsevier BV
Herausgeber URL
http://dx.doi.org/10.1016/j.biomaterials.2016.02.042
Datum der Datenerfassung
2023
Titel
Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols
Ausgabe der Zeitschrift
89

Data source: Crossref

Other metadata sources:
Abstract
We introduce active surfaces generated by immobilizing protein-polymer nanoreactors on a solid support for sensitive sugar alcohols detection. First, such selective nanoreactors were engineered in solution by simultaneous encapsulation of specific enzymes in copolymer polymersomes, and insertion of membrane proteins for selective conduct of sugar alcohols. Despite the artificial surroundings, and the thickness of the copolymer membrane, functionality of reconstituted Escherichia coli glycerol facilitator (GlpF) was preserved, and allowed selective diffusion of sugar alcohols to the inner cavity of the polymersome, where encapsulated ribitol dehydrogenase (RDH) enzymes served as biosensing entities. Ribitol, selected as a model sugar alcohol, was detected quantitatively by the RDH-nanoreactors with GlpF-mediated permeability in a concentration range of 1.5-9 mM. To obtain "active surfaces" for detecting sugar alcohols, the nanoreactors optimized in solution were then immobilized on a solid support: aldehyde groups exposed at the compartment external surface reacted via an aldehyde-amino reaction with glass surfaces chemically modified with amino groups. The nanoreactors preserved their architecture and activity after immobilization on the glass surface, and represent active biosensing surfaces for selective detection of sugar alcohols, with high sensitivity.
Addresses
  • Chemistry Department, University of Basel, Klingelbergstrasse 80, Basel, Switzerland.
Autoren
  • Xiaoyan Zhang
  • Mihai Lomora
  • Tomaz Einfalt
  • Wolfgang Meier
  • Noreen Klein
  • Dirk Schneider
  • Cornelia G Palivan
DOI
10.1016/j.biomaterials.2016.02.042
eISSN
1878-5905
Externe Identifier
  • PubMed Identifier: 26950167
Funding acknowledgements
  • Stiftung Rheinland-Pfalz für Innovation: 977
  • German Chemical Industry:
  • Swiss National Science Foundation: 140303/1
  • National Centre of Competence in Research:
  • Swiss Nanoscience Institute: DCH2130
Open access
false
ISSN
0142-9612
Zeitschrift
Biomaterials
Schlüsselwörter
  • Escherichia coli
  • Sugar Alcohols
  • Ribitol
  • Polymers
  • Sugar Alcohol Dehydrogenases
  • Aquaporins
  • Escherichia coli Proteins
  • Biosensing Techniques
  • Permeability
  • Surface Properties
  • Models, Molecular
  • Nanostructures
  • Immobilized Proteins
Sprache
eng
Medium
Print-Electronic
Online publication date
2016
Paginierung
79 - 88
Datum der Veröffentlichung
2016
Status
Published
Datum der Datenerfassung
2016
Titel
Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols.
Sub types
  • Research Support, Non-U.S. Gov't
  • Journal Article
Ausgabe der Zeitschrift
89

Data source: Europe PubMed Central

Abstract
We introduce active surfaces generated by immobilizing protein-polymer nanoreactors on a solid support for sensitive sugar alcohols detection. First, such selective nanoreactors were engineered in solution by simultaneous encapsulation of specific enzymes in copolymer polymersomes, and insertion of membrane proteins for selective conduct of sugar alcohols. Despite the artificial surroundings, and the thickness of the copolymer membrane, functionality of reconstituted Escherichia coli glycerol facilitator (GlpF) was preserved, and allowed selective diffusion of sugar alcohols to the inner cavity of the polymersome, where encapsulated ribitol dehydrogenase (RDH) enzymes served as biosensing entities. Ribitol, selected as a model sugar alcohol, was detected quantitatively by the RDH-nanoreactors with GlpF-mediated permeability in a concentration range of 1.5-9 mM. To obtain "active surfaces" for detecting sugar alcohols, the nanoreactors optimized in solution were then immobilized on a solid support: aldehyde groups exposed at the compartment external surface reacted via an aldehyde-amino reaction with glass surfaces chemically modified with amino groups. The nanoreactors preserved their architecture and activity after immobilization on the glass surface, and represent active biosensing surfaces for selective detection of sugar alcohols, with high sensitivity.
Date of acceptance
2016
Autoren
  • Xiaoyan Zhang
  • Mihai Lomora
  • Tomaz Einfalt
  • Wolfgang Meier
  • Noreen Klein
  • Dirk Schneider
  • Cornelia G Palivan
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/26950167
DOI
10.1016/j.biomaterials.2016.02.042
eISSN
1878-5905
Zeitschrift
Biomaterials
Schlüsselwörter
  • Active surfaces
  • Amphiphilic triblock copolymers
  • Membrane permeability
  • Membrane proteins
  • Nanoreactors
  • Selectivity
  • Aquaporins
  • Biosensing Techniques
  • Escherichia coli
  • Escherichia coli Proteins
  • Immobilized Proteins
  • Models, Molecular
  • Nanostructures
  • Permeability
  • Polymers
  • Ribitol
  • Sugar Alcohol Dehydrogenases
  • Sugar Alcohols
  • Surface Properties
Sprache
eng
Country
Netherlands
Paginierung
79 - 88
PII
S0142-9612(16)00164-2
Datum der Veröffentlichung
2016
Status
Published
Datum, an dem der Datensatz öffentlich gemacht wurde
2016
Titel
Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols.
Sub types
  • Journal Article
  • Research Support, Non-U.S. Gov't
Ausgabe der Zeitschrift
89

Data source: PubMed

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